NEK6 antibody

Principal name

NEK6 antibody

Alternative names for NEK6 antibody

NimA-related protein kinase 6, Protein kinase SID6-1512, Serine/threonine-protein kinase Nek6

SwissProt ID

Q9HC98 (Human)

Gene ID

10783 (NEK6)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP)

Background of NEK6 antibody

The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain. The protein is believed to regulate chromatin condensation in mammalian cells via phosphorylation of histones H1 and H3, and it has been shown to phosphorylate ribosomal S6 protein kinase (S6K1) and serum and glucocorticoid induced protein kinase (SGK1), which regulate diverse cellular processes. The protein also has been shown to bind NEK9, another NIMA-related kinase which regulates chromosome alignment and segregation. At least three mRNA transcripts have been identified, 1.6-, 2.6-, and 9.5-kb. The NEK6 gene is localized to 9q33-34, a region at which the loss of heterozygosity is associated with transitional cell carcinomas. NEK6 expression has been documented in many normal human tissues, including blood, brain, heart, liver, lung, skeletal muscle, spleen and testis. ESTs have been isolated from numerous tissue libraries, especially normal cervix and amnion and cancerous bone marrow.

General readings

Lee,E.J., (2007) Biochem. Biophys. Res. Commun. 358 (3), 783-788

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEK6 antibody

Anti-NEK6 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

NEK6 antibody

Anti-NEK6 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

NEK6 antibody

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-NEK6mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500511, Dilution 1:50) Mouse IgG1 OTI8G5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NEK6 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500529) Mouse IgG1 OTI5B9 Purified Hu ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NEK6 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500545) Mouse IgG1 OTI5D7 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NEK6 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500551) Mouse IgG1 OTI2H7 Purified Hu IP, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

NEK6 antibody

Immunohistochemistry-Paraffin: NEK6 Antibody (5D7) [NBP1-47865] - Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-NEK6mouse monoclonal antibody. (Dilution 1:50) Mouse IgG1 5D7 Aff - Purified Hu F, ICC/IF, P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

NEK6 antibody

NEK6 polyclonal antibody (A01), Lot # 051011JC01. Western Blot analysis of NEK6 expression in human ovary cancer. Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NEK6 antibody

NEK6 Mouse IgG2a 3B5 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEK6 antibody

NEK6 Mouse IgG2a 2A7 Purified Hu E
50 µg / €370.00
  Abnova Taiwan Corp.

NEK6 antibody

NEK6 Mouse IgG2b 4B10 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

NEK6 antibody

Human Lung; WB Suggested Anti-NEK6 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Lung; NEK6 antibody - N-terminal region (ARP48756_P050) in Human Lung cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

NEK6 antibody

Purkinje Neurons Rabbit Aff - Purified Hu P
50 µl / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NEK6 (transcript variant 2)

NEK6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NEK6 (transcript variant 1)

NEK6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NEK6 (transcript variant 5)

NEK6 Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NEK6

NEK6 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

NEK6

NEK6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NEK6

NEK6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

NEK6

NEK6 Human
5 µg / €510.00
  Abnova Taiwan Corp.

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