NEK9 antibody

Principal name

NEK9 antibody

Alternative names for NEK9 antibody

Never in mitosis A-related kinase 9, Nercc1 kinase, KIAA1995, NEK8, NERCC, NIMA-related kinase 8

SwissProt ID

Q7ZZC8 (Xenla), Q8K1R7 (Mouse), Q8TD19 (Human)

Gene ID

91754 (NEK9)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bt (Bat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of NEK9 antibody

Nek8 (also designated never in mitosis A (NimA)-related kinase 8, Jck and NEK12A) is a mitotic regulator that plays a significant role in maintaining renal tubular integrity. Nek8 transcript is abundant in thyroid, adrenal gland and skin, with overexpression in breast tumors and infiltrating ductal carcinomas, and moderate levels in mucinous adenocarcinoma. Nek8 mutations correlate to juvenile autosomal recessive polycystic kidney disease in humans. Nek9 (never in mitosis A (NimA)-related kinase 9, Nercc1 kinase) regulates G1/S transition and S phase progression by influencing spindle dynamics and chromosome separation. Nek9 phosphorylates different histones (Histone H3 on serine and threonine), myelin basic protein, β-casein (serine) and BICD2. Nek9 interacts with Ran GTPase, Nek6, Nek7, BICD2, SSRP1 and SUPT16H/ FACT complex. Nek9 transcript is abundant in heart, liver, kidney and testis. Nek family cell-cycle protein kinases in humans have at least 11 members that share an amino-terminal catalytic domain related to NIMA, an Aspergillus nidulans kinase that plays on several aspects of mitosis, with divergent carboxyl-terminal tails of varying length.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEK9 antibody

  Rabbit polyclonal NEK9 (Ab-210) antibody  
Western blot analysis of extracts from A549 cells, using NEK9 (Ab-210) antibodyThe lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NEK9 antibody

  Rabbit Polyclonal anti-NEK9 Antibody  
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-NEK9 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6241 Purified Hu IP, P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

NEK9 (Kinase Dom.) antibody

  Rabbit Polyclonal Anti-NEK9 Antibody (Kinase Domain)  
NEK9 Rabbit Aff - Purified Hu, Ms P
50 µg / €400.00
  OriGene Technologies, Inc.

NEK9 (P/S/T-rich Dom.) antibody

  Rabbit Polyclonal Anti-NEK9 Antibody (Pro/Ser/Thr Rich Domain)  
NEK9 Rabbit Aff - Purified Hu P
50 µg / €400.00
  OriGene Technologies, Inc.

NEK9 antibody

  Rabbit Polyclonal Anti-NEK9 Antibody (Pro/Ser/Thr Rich Domain)  
Anti-NEK9 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Bov, Bt, Can, Hu, Ms, Rt
50 µg / €400.00
  OriGene Technologies, Inc.

NEK9 antibody

  Rabbit Polyclonal Anti-NEK9 Antibody  
WB Suggested Anti-NEK9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NEK9 antibody

  Rabbit Polyclonal Anti-NEK9 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (NEK9 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NEK9 antibody

  NEK9 mouse monoclonal antibody, clone OTI1H1 (formerly 1H1)  
Anti-NEK9 mouse monoclonal antibody (TA503326) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9(RC211326). Mouse IgG1 OTI1H1 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NEK9 antibody

  NEK9 mouse monoclonal antibody, clone OTI7G7 (formerly 7G7)  
Anti-NEK9 mouse monoclonal antibody (TA503367) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9(RC211326). Mouse IgG1 OTI7G7 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

NEK9 antibody

Brain, cortex Rabbit IgG Aff - Purified Hu IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

NEK9 antibody

Western blot analysis of Nek9 antibody (AP20364PU-N) in extracts from A549 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NEK9 pThr210 antibody

Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech

NEK9 pThr210 antibody

Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €330.00
  Assay Biotech

NEK9 antibody

Western blot analysis of extracts from A549 cells, using NEK9 (Ab-210) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

NEK9 antibody

Western blot analysis of extracts from A549 cells, using NEK9 (Ab-210) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

NEK9 - human gene knockout kit via CRISPR

NEK9 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NEK9 - human gene knockout kit via CRISPR

NEK9 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NEK9 - human gene knockout kit via CRISPR

NEK9 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NEK9 - human gene knockout kit via CRISPR

NEK9 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NEK9

  Recombinant protein of human NIMA (never in mitosis gene a)- related kinase 9 (NEK9)  
NEK9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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