Nephrin antibody

Principal name

Nephrin antibody

Alternative names for Nephrin antibody

NPHS1, NPHN

SwissProt ID

O60500 (Human)

Gene ID

4868 (NPHS1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Guinea Pig, Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Selected product citations ‐ of BP5030

1. Boini KM, Amann K, Kempe D, Alessi DR, Lang F. Proteinuria in mice expressing PKB/SGK-resistant GSK3. Am J Physiol Renal Physiol. 2009 Jan;296(1):F153-9. doi: 10.1152/ajprenal.90398.2008. Epub 2008 Nov 5. PubMed PMID: 18987114.
2. Huber TB, Hartleben B, Winkelmann K, Schneider L, Becker JU, Leitges M, et al. Loss of podocyte aPKClambda/iota causes polarity defects and nephrotic syndrome. J Am Soc Nephrol. 2009 Apr;20(4):798-806. doi: 10.1681/ASN.2008080871. Epub 2009 Mar 11. PubMed PMID: 19279126.
3. Chittiprol S, Chen P, Petrovic-Djergovic D, Eichler T, Ransom RF. Marker expression, behaviors, and responses vary in different lines of conditionally immortalized cultured podocytes. Am J Physiol Renal Physiol. 2011 Sep;301(3):F660-71. doi: 10.1152/ajprenal.00234.2011. Epub 2011 Jun 1. PubMed PMID: 21632959.
4. Thomasova D, Bruns HA, Kretschmer V, Ebrahim M, Romoli S, Liapis H, et al. Murine Double Minute-2 Prevents p53-Overactivation-Related Cell Death (Podoptosis) of Podocytes. J Am Soc Nephrol. 2015 Jul;26(7):1513-23. doi: 10.1681/ASN.2014040345. Epub 2014 Oct 27. PubMed PMID: 25349197.
5. Okamoto K, Honda K, Doi K, Ishizu T, Katagiri D, Wada T, et al. Glypican-5 Increases Susceptibility to Nephrotic Damage in Diabetic Kidney. Am J Pathol. 2015 Jul;185(7):1889-98. doi: 10.1016/j.ajpath.2015.03.025. Epub 2015 May 15. PubMed PMID: 25987249.
6. Kokkinopoulou M, Spiecker L, Messerschmidt C, Barbeck M, Ghanaati S, Landfester K, et al. On the Ultrastructure and Function of Rhogocytes from the Pond Snail Lymnaea stagnalis. PLoS One. 2015 Oct 21;10(10):e0141195. doi: 10.1371/journal.pone.0141195. eCollection 2015. PubMed PMID: 26488403.
7. Ramaiah, V;Kumar, S. Extracellular histones cause vascular necrosis in severe glomerulonephritis. Thesis 2015. https://edoc.ub.uni-muenchen.de/18767/1/Vankayala_Ramaiah_Santhosh_Kumar.pdf
8. Mulay SR, Romoli S, Desai J, Honarpisheh MM, Kumar SV, Anders HJ, et al. Murine Double Minute-2 Inhibition Ameliorates Established Crescentic Glomerulonephritis. Am J Pathol. 2016 Jun;186(6):1442-53. doi: 10.1016/j.ajpath.2016.01.017. Epub 2016 Apr 18. PubMed PMID: 27102769. 
9.Saito, R;Rocanin-Arjo, A;You, YH;Darshi, M;Van Espen, B;Miyamoto, S;Pham, J;Pu, M;Romoli, S;Natarajan, L;Ju, W;Kretzler, M;Nelson, R;Ono, K;Thomasova, D;Mulay, SR;Ideker, T;D'Agati, V;Beyret, E;Belmonte, JC;Anders, HJ;Sharma, K;2016Systems biology analysis reveals role of MDM2 in diabetic nephropathy. JCI Insighte 2016, e87877. 1, 17. PubMed PMID: 27777973.

Background of Nephrin antibody

Nephrin was first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE). Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.
Primary steroid resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss. Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2. Nephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Recent studies have suggested that mutations in the gene for Nephrin reportedly lead to congenital nephrosis. Three novel podocyte proteins, Podocin, Nephrin and alpha Actinin 4 have been identified in congenital and experimental models of proteinuria. The role of Nephrin in anti apoptotic activity in podocyte slit diaphragm is believed to be associated with vascular endothelial derived growth factors VEGF signaling.

General readings

Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein - Nephrin - is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nephrin (1243-1256) antibody

Staining for Nephrin on formalin fixed paraffin embedded kidney tissue sections using Acris Cat.-No BP5030 anti-Nephrin serum following antigen retrieval. Indirect immunoperoxidase staining. DAB substrate, hematoxylene counterstain. Guinea Pig Serum Hu, Ms ICC/IF, P, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

Nephrin antibody

Western blot analysis of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nephrin (Cys465-Cys528) antibody

Western blot analysis of Mouse Nephrin: Lane 1:  50ng; Lane 2: 100ng, Lane 3: 200ng of recombinant Mouse Nephrin (extracellular domain, Gln37 – Thr 1049; cat.#:3159/NN, R&D systems). Rabbit Y17-R Aff - Purified Hu, Ms, Rt E, IP, P, WB
0.1 ml / €370.00
  DB Biotech, spol. s r.o.

Sample sizes available

Nephrin (Cys465-Cys528) antibody

Western blot analysis of Mouse Nephrin: Lane 1:  50ng; Lane 2: 100ng, Lane 3: 200ng of recombinant Mouse Nephrin (extracellular domain, Gln37 – Thr 1049; cat.#:3159/NN, R&D systems). Rabbit Y17-R Aff - Purified Hu, Ms, Rt E, IP, P, WB
50 µl / €260.00
  DB Biotech, spol. s r.o.

Sample sizes available

Nephrin antibody

Immunohistochemical staining of human kidney (Formalin-fixed, Paraffin-embedded) using  Nephrin antibody Cat.-No. AP15677PU. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

Nephrin antibody

Immunohistochemical staining of human kidney (Formalin-fixed, Paraffin-embedded) using  Nephrin antibody Cat.-No. AP15677PU. Rabbit Purified Hu P
1 ml / €530.00
  Acris Antibodies GmbH

Nephrin antibody

Immunohistochemical staining of human kidney (Formalin-fixed, Paraffin-embedded) using  Nephrin antibody Cat.-No. AP15677PU. Rabbit Purified Hu P
0.1 ml / €200.00
  Acris Antibodies GmbH

Nephrin (Center) antibody

Immunohistochemistry of Nephrin in human kidney tissue with Nephrin antibody at 5 ug/mL. Rabbit IgG Aff - Purified Hu E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nephrin antibody

Predicted band size: 135 kDa . Positive control: Mouse spleen and kidney tissue lysate. Recommended dilution: 1/500-2000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nephrin antibody

Predicted band size: 135 kDa . Positive control: Mouse kidney tissue lysate. Recommended dilution: 1/500-2000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nephrin antibody

Human Kidney stained with anti-Nephrin antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nephrin (Cys53-Cys101) antibody

Western blot analysis of Mouse Nephrin using Nephrin Antibody (DB 024): Lane 1: 50ng, Lane 2: 100ng, Lane 3 –
200ng of recombinant Mouse Nephrin. Rabbit G17-H Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 ml / €570.00
  DB Biotech, spol. s r.o.

Sample sizes available

Nephrin (Cys53-Cys101) antibody

Western blot analysis of Mouse Nephrin using Nephrin Antibody (DB 024): Lane 1: 50ng, Lane 2: 100ng, Lane 3 –
200ng of recombinant Mouse Nephrin. Rabbit G17-H Aff - Purified Hu, Ms, Rt E, IP, WB
50 µl / €370.00
  DB Biotech, spol. s r.o.

Sample sizes available

Nephrin (1218-1234) antibody

Kidney, glomerulus: Formalin-Fixed Paraffin-Embedded (FFPE) using NPHS1 Antibody Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

Nephrin (C-term) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Nephrin antibody

Immunohistochemistry (IHC) analyzes of Nephrin antibody (Cat.-No.: AP20587PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

Nephrin (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human Kidney stained with Nephrin antibody Cat.-No AP23552PU-N at 5 µg/ml after heat-induced antigen retrieval. Rabbit Hu P
50 µg / €470.00
  Acris Antibodies GmbH

Nephrin Control Peptide

Nephrin
50 µg / €160.00
  Acris Antibodies GmbH

Nephrin Control Peptide

Nephrin
50 µg / €160.00
  Acris Antibodies GmbH

Nephrin (Center) Control Peptide

Nephrin
50 µg / €160.00
  Acris Antibodies GmbH

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