Nesprin-3 antibody

Principal name

Nesprin-3 antibody

Alternative names for Nesprin-3 antibody

C14orf49

SwissProt ID

Q4FZC9 (Mouse), Q6ZMZ3 (Human)

Gene ID

161176 (SYNE3), 212073 (4831426I19Rik)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP)

Background of Nesprin-3 antibody

Nesprin-3, is a member of the nesprin family and is a transmembrane protein of the outer nuclear membrane (ONM). Like Nesprin-1 and Nesprin-2, Nesprin-3 also contains a COOH-terminal klarsicht/ANC-1/syne (KASH) domain and a series of spectrin repeats. In humans, the full length  nesprin-1 and nesprin-2 are giant proteins, containing both an actin-binding domain resulting in molecular masses of respectively 976 and 764 kDa.
These proteins are therefore suggested to be involved in nuclear positioning. Nesprin-3, in contrast to Nesprin-1 and Nesprin-2, lacks the actin-binding domain and cannot bind directly to the cytoskeleton. Nesprin-3 has two isoforms, Nesprin-3a and Nesprin-3b, which differ in the N-terminus. The N-terminus of Nesprin-3a, but not that of Nesprin-3b interacts with the cytoskeletal crosslinker protein Plectin.
Plectin influences the structural organization and integrity of the cytoskeleton and is thought to assist in the mechanical strengthening of the cells. Plectin binds to nesprin-3a and to the intermediate filaments through its C-terminus, suggesting that the interaction between nesprin-3a and plectin links the nucleus to the intermediate filament system.  Nesprin-3, like Nesprin-1 and Nesprin-2 is localized in the nuclear envelope.
This localization is due to its interaction with SUN proteins, both SUN-1 and SUN-2, present in the inner nuclear membrane.

11 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nesprin-3 antibody

Western blot analysis of extracts from Jurkat cells, using C14orf49 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nesprin-3 (Alpha) antibody

Immunofluorescence staining of a cultured Mouse embryonic fibroblast with Nsp3 for nesprin-3a (green), and counter-staining of nuclear DNA with DAPI (blue). Mouse IgG1 Nsp-3 Purified Ms C, ICC/IF, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Nesprin-3 (169-218) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Nesprin-3 (C-term) antibody

SYNE3 antibody (C-term) (Cat. #AP54124PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human hodgkin lymphoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYNE3 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Nesprin-3 antibody

Western blot analysis of extracts from Jurkat cells, using C14orf49 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

Nesprin-3 antibody

Western blot analysis of extracts from Jurkat cells, using C14orf49 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

Nesprin-3 antibody

Immunofluorescent analysis of SYNE3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Nesprin-3 antibody

Immunofluorescent analysis of SYNE3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Nesprin-3 antibody

Immunofluorescent analysis of SYNE3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Nesprin-3 antibody

Nesprin 3 Antibody Rabbit Serum Ms ICC/IF, IP, WB
0.1 ml / €400.00
  Immuquest

Lysates

Catalog No.    

SYNE3 overexpression lysate

SYNE3 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

11 Item(s)

per page
  • LinkedIn