Nestin antibody

Principal name

Nestin antibody

Alternative names for Nestin antibody

NES, Nbla00170

SwissProt ID

P21263 (Rat), P48681 (Human), Q6P5H2 (Mouse)

Gene ID

10763 (NES), 18008 (Nes), 25491

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Hst (Hamster), Macaque, Rb (Rabbit), GP (Guinea Pig), Eq (Equine)

Available hosts

Chicken, Goat, Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Immunoelectron Microscopy (EM), Immunocytochemistry (ICC)

Background of Nestin antibody

Nestin is a large intermediate filament protein originally identified by Hockfield and McKay (1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. 
This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.

General readings

Messam, C.A., et al. Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody. Exp. Neurology. 161: 585-596, 2000. (Western blot, Immunocytochemistry, human)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nestin antibody

Western blot analysis of Nestin Antibody (S1409) (Cat.#TA324694) in HepG2 cell line lysates (35ug/lane). NES (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Nestin (C-term) antibody

Human kidney stained with anti-Nestin antibody AM20271PU Rabbit IgG SP103 Purified Hu P, WB
0.5 ml / €510.00
  Acris Antibodies GmbH

Nestin (C-term) antibody

Human kidney stained with anti-Nestin antibody AM20271PU Rabbit IgG SP103 Purified Hu P, WB
1 ml / €730.00
  Acris Antibodies GmbH

Nestin (C-term) antibody

Human kidney stained with anti-Nestin antibody AM20271PU Rabbit IgG SP103 Purified Hu P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

Nestin antibody

Human liver carcinoma stained with anti-nestin antibody Rabbit IgG Aff - Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

Nestin antibody

Human liver carcinoma stained with anti-nestin antibody Rabbit IgG Aff - Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

Nestin antibody

Human liver carcinoma stained with anti-nestin antibody Rabbit IgG Aff - Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

Nestin antibody

AP23802PU Nestin antibody staining of Human Kidney. Rabbit IgG Aff - Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

Nestin antibody

AP23802PU Nestin antibody staining of Human Kidney. Rabbit IgG Aff - Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

Nestin antibody

AP23802PU Nestin antibody staining of Human Kidney. Rabbit IgG Aff - Purified P
0.1 ml / €190.00
  Acris Antibodies GmbH

Nestin antibody

Immunofluorescence: Nestin Antibody (4D11) - Mixed cultures of neonatal rat neurons and glia stained with anti-Nestin (red), chicken antibody to Vimentin  and DNA (DAPI stain, blue). Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the Nestin (4D11) antibody. The filamentous staining pattern is as expected as both Nestin and Vimentin are components of 10nm filaments. Mouse IgG1 4D11 Purified Hu, Ms, Rt ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nestin antibody

Western Blot: Nestin Antibody (10C2) - Detection of human nestin using anti-Nestin.  Lane 1: 5 ug total rat brain protein, lane 2: 5 ug human CNS progenitor cell protein. Mouse IgG1 10C2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nestin antibody

Detection of nestin protein in human fetal temporal lobe tissue lysates (30 ug).  Lane 1: 0.5 ug/ml TA309764: lane 2: 2 ug/ml TA309764.  ECL: 1 second exposure. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nestin antibody

Western blot analysis of SGC cells (30ug lysate) with anti-Nestin antibody diluated at 1:1000. (Predicted and observed size: ~170kDa) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nestin (1484-1500) antibody

WB using Anti-Nestin antibody shows detection of a band ~220 kDa corresponding to human Nestin (arrowhead).  Undifferentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 1), or differentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 2) were separated by SDS-PAGE using 4-20% gradient gel.The primary antibody diluted to 1:2,000. Peroxidase conjugated Gt-a-Rabbit IgG [H&L] was used at 1:10000. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nestin antibody

U251 cell lysates prepared under non-reducing (lane 1) or reducing (lane 2) conditions were resolved by SDS-PAGE then immunoblotted with 2 ug/ml of Anti-Human Nestin Purified. Bands were visualized using Anti-Mouse IgG HRP. Mouse 10C2 Purified Hu P, WB
25 µg / €120.00
  OriGene Technologies, Inc.

Nestin antibody

WB Suggested Anti-NES Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: OVCAR-3 cell lysate Rabbit IgG Purified Can, Eq, Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Nestin antibody

ELISA: Nestin Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nestin antibody

Immunostaining of intracellular Nestin filaments in U251 cells Mouse IgG1 10C2 Purified Hu C, ICC/IF, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Nestin antibody

Immunostaining of intracellular Nestin filaments in U251 cells Mouse IgG1 10C2 Supernatant Hu C, ICC/IF, P, WB
1 ml / €250.00
  Acris Antibodies GmbH

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