Netrin-1 antibody

Principal name

Netrin-1 antibody

Alternative names for Netrin-1 antibody

NTN1, NTN1L

SwissProt ID

O09118 (Mouse), O95631 (Human), Q924Z9 (Rat)

Gene ID

9423 (NTN1), 18208 (Ntn1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Fe (Feline), Mky (Monkey), Rt (Rat), Can (Canine), Macaque, Chk (Chicken)

Available hosts

Chicken, Mouse, Rabbit, Goat, Rat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of Netrin-1 antibody

Semaphorins, neuropilins and netrins are among a number of molecules and their receptors that regulate the developing nervous system to guide the development of neural circuits.1 Although first identified as axon guidance cues,2,3 it is now apparent that many of these same factors are not limited to the guidance of growing axons, but have roles in a range of processes from the guidance of cell migration to the regulation of the immune response, angiogenesis, lung branching morphogenesis, nervous system regeneration, and cancer.4-9 The semaphorins make up the largest family of axon guidance cues. They are characterized by the presence of an approximately 500 amino acid N-terminal semaphorin (Sema) domain. Semaphorins function mainly as chemorepellents that direct axons away from tissues.3 Semaphorin 3A (Sema3A) has been shown to be repellent to cortical axons and to inhibit axon branching.10 The transmembrane protein semaphorin 6A has been shown to repel embryonic sympathetic axons.11 The actions of the various semaphorins are not always similar. Semaphorin 3A has been found to inhibit tumor development whereas semaphorin 6A may contribute to tumor progression.9 Neuropilins are the ligand binding moieties in the class 3 Semaphorin receptor complexes that subsequently activate signaling through associated plexins. Two types have been identified so far: Neuropilin-1 (Npn-1) and Neuropilin-2 (Npn-2) receptors. At the amino acid sequence level, Npn-1 and Npn-2 share 44% identity. Npn-1 and Npn-2 show different expression patterns in developing neurons of the central and peripheral nervous systems, and show different binding specificities for different members of he semaphorin family. Both also function as receptors for some forms of vascular endothelial growth factor (VEGF).12 Netrins are a family of laminin-related small proteins that are involved in axon guidance and eurite outgrowth. Netrin-1 has been shown to attract cortical growth cones and promote axon branching.10 Netrin-4 (first named b-netrin) was found to promote neurite elongation from olfactory bulb explants.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Netrin-1 (C-term) antibody

TA305829 (0.5ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Netrin-1 antibody

NTN1 Antibody (C-term) (Cat. #TA325129) western blot analysis in U87-MG cell line lysates (35ug/lane).This demonstrates the NTN1 antibody detected the NTN1 protein (arrow). Rabbit IgG Purified Chk, Hu, Ms, Por WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Netrin-1 Control Peptide

Netrin-1 Purified
50 µg / €210.00
  Acris Antibodies GmbH

Netrin-1 antibody

Figure 1. Western blot using AP05263PU-N , Rabbit Polyclonal at 0.05 µg/ml on MDCKII cell extract (10 µg/lane). Blots were developed with goat anti rabbit Ig (1:75k) and Pierce's Supersignal West Femto system. Rabbit IgG Aff - Purified Bov, Fe, Hu, Mky, Ms, Rt WB
25 µg / €480.00
  Acris Antibodies GmbH

Netrin-1 antibody

Western blot using AP05263SU-N, rabbit polyclonal at 5 µg/ml on MDCKII cell extract (10 µg/lane). Blots were developed with goat anti rabbit Ig (1:100k) and Pierce's Supersignal West Femto system. Rabbit IgG Purified Bov, Fe, Hu, Mky, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Netrin-1 Control Peptide

Netrin-1
0.25 mg / €250.00
  Acris Antibodies GmbH

Netrin-1 antibody

Western blot using Netrin-1  antibody (AP10176PU-N at 1/500 dilution). Rabbit Aff - Purified Hu E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Netrin-1 antibody

Immunohistochemistry analysis of Netrin-1 antibody (Cat.-No AP20679PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Netrin-1 (498-509) antibody

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bov, Elephant, Eq, Fi, Hst, Hu, L, Mky, Ms, Op, Por, Prim, Rt, Xen P
50 µg / €470.00
  Acris Antibodies GmbH

Netrin-1 antibody

Dissociated cell cultures of an e13 Mouse brain showing Netrin-1 (green staining) in neuronal precursor cells. Chicken Ig Aff - Purified Hu, Ms ICC/IF, P
0.1 mg / €440.00
  Acris Antibodies GmbH

Netrin-1 antibody

Netrin-1 Mouse IgG2a 5H8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Netrin-1 antibody

Western blot using NTN1 polyclonal antibody ( Cat # PAB10137 ) at 5 µg/ml on MDCK II cell extract (10 µg/lane). Bov, Hu, Macaque, Ms, Rt
0.1 mg / €400.00
  Abnova Taiwan Corp.

Netrin-1 antibody

Netrin-1 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

Netrin-1 antibody

Immunohistochemical analysis of Netrin 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Netrin-1 antibody

Immunohistochemical analysis of Netrin 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Netrin-1 antibody

Immunohistochemical analysis of Netrin 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Netrin-1 antibody

Dissociated cell cultures of an e13 mouse brain showing Netrin-1 (green staining) in neuronal precursor cells. Chicken Aff - Purified Hu, Ms C, ICC/IF, WB
0.1 ml / €180.00
  Neuromics Antibodies

Netrin-1 antibody

Dissociated cell cultures of an e 13 mouse brain showing Netrin-1 (green staining) in neuronal precursor cells. Chicken Aff - Purified Hu, Ms ICC/IF, P
0.5 ml / €380.00
  Novus Biologicals Inc.

Netrin-1 antibody

Netrin-1 Mouse 158936 Purified Ms E, WB
0.1 mg / €340.00
  Novus Biologicals Inc.

Netrin-1 antibody

Netrin-1 Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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