Netrin receptor DCC antibody

Principal name

Netrin receptor DCC antibody

Alternative names for Netrin receptor DCC antibody

IGDCC1, Tumor suppressor protein DCC, Colorectal cancer suppressor, CRC18, CRCR1, Immunoglobulin superfamily DCC subclass member 1

SwissProt ID

P43146 (Human), P70211 (Mouse)

Gene ID

1630 (DCC)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Functional assay (FN), Paraffin Sections (P), Frozen Sections (C)

Background of Netrin receptor DCC antibody

Immunoglobulin superfamily DCC subclass member 1/P43146 Deleted in Colorectal Carcinoma protein (DCC) and Neogenin are receptors for Netrin 1 in developing brain. DCC is a tumor suppressor gene as well as a conditional oncogene (1). When DCC is bound to its ligand, netrin, it acts as oncogene and activates the pathways that promote cell survival and nuerite growth down-stream to ligand concentration. Halo DCC promotes apoptotic pathways (2,3). When DCC is not bound to netrin 1 it can initiate apoptosis without the formation of an apoptosome or cytochrome c release (4). DCC has recently been found to suppress metastasis in vitro on cells. DCC trans-membrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. DCC is a 1447 aa protein resides on chromosome 18q21. DCC is expressed in low abundance ubiquteously and at higher levels in many areas of the brain, particularly in dopaminergic neurons. It is found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Higher levels of DCC are found in neonatal tissues and its expression is significantly reduced in adult hood (3). The DCC is expressed on developing spinal cord neurons to promote axonal and nuerite growth in response to netrin. DCC and neogenin, two of the netrin-1 receptors, have recently been shown to have sites for tyrosine phosphorylation (at Y1420 on DCC) and are likely interacting with Src family kinases in regulating responses to netrin-1(5). The DCC undergoes proteolytic degradation to yield a 120 kDa fragment in apoptotic cells (6). The DCC-selective antibodies were generated against antigenic peptide taken from human DCC protein corresponding near to carboxy terminal end of DCC protein. DCC- antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.

General readings

General / background references:Huerta S et al. Human colon cancer cells deficient in DCC produce abnormal transcripts in progression of carcinogenesis. Dig Dis Sci 46:1884-91 (2001). PubMed PMID: 11575440
Cooper HM et al. Cloning of the mouse homologue of the deleted in colorectal cancer gene (mDCC) and its expression in the developing mouse embryo. Oncogene 11:2243-54 (1995). PubMed PMID: 8570174
Fearon ER et al. Identification of a chromosome 18q gene that is altered in colorectal cancers. Science 247:49-56 (1990). PubMed PMID: 2294591

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Netrin receptor DCC antibody

Immunofluorescence analysis of LOVO cells, using DCC antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

Netrin receptor DCC antibody

WB Suggested Anti-DCC Antibody Titration: 0.2-1 ug/ml; Positive Control: ACHN cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Netrin receptor DCC antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCC (RC218506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCC. Mouse IgG2b OTI4A1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Netrin receptor DCC antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCC (RC218506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCC. Mouse IgG1 OTI5D10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Netrin receptor DCC (C-term) antibody

Netrin receptor DCC Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Netrin receptor DCC (1437-1447) antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Can, Elephant, Eq, Fi, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt, Xen P
50 µg / €470.00
  Acris Antibodies GmbH

Netrin receptor DCC (Center) antibody

Formalin fixed and paraffin embedded human stomach tissue stained with Netrin receptor DCC Antibody Cat.-No AP51195PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Netrin receptor DCC antibody

Immunofluorescence analysis of LOVO cells, using DCC Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

Netrin receptor DCC antibody

Immunofluorescence analysis of LOVO cells, using DCC Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

Netrin receptor DCC (Center) antibody

Human ACHN; WB Suggested Anti-DCC Antibody Titration: 0.2-1 ug/ml. Positive Control: ACHN cell lysate; DCC antibody - middle region (ARP46583_P050) in Human ACHN cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Netrin receptor DCC antibody

Netrin receptor DCC Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Netrin receptor DCC antibody

Western blot analysis of DCC expression in ACHN (A) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Netrin receptor DCC antibody

Western blot analysis of DCC expression in ACHN (A) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Netrin receptor DCC antibody

Western blot analysis of DCC expression in ACHN (A) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Netrin receptor DCC antibody

Netrin receptor DCC Goat IgG Aff - Purified Ms E, FN, WB
25 µg / €380.00
  Novus Biologicals Inc.

Netrin receptor DCC antibody

Netrin receptor DCC Mouse IgG1 AF5 Aff - Purified Hu ICC/IF, IP, WB
50 µg / €390.00
  Novus Biologicals Inc.

Netrin receptor DCC antibody

Western Blot: DCC Antibody [NBP1-56988] - This Anti-DCC antibody was used in Western Blot of ACHN       tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Netrin receptor DCC antibody

Netrin receptor DCC Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €360.00
  Novus Biologicals Inc.

Netrin receptor DCC antibody

Netrin receptor DCC Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Netrin receptor DCC

Netrin receptor DCC Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

20 Item(s)

per page
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