NEUROD1 antibody

Principal name

NEUROD1 antibody

Alternative names for NEUROD1 antibody

NEUROD1, NEUROD, Neurogenic differentiation factor 1

SwissProt ID

P79765 (Chick), Q13562 (Human), Q60867 (Mouse), Q64289 (Rat)

Gene ID

4760 (NEUROD1), 3476028 (accD)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Fi (Fish), Xen (Xenopus), Ze (Zebrafish), Chk (Chicken), African clawed frog, Eq (Equine), Por (Porcine), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of NEUROD1 antibody

The NEUROD1 gene encodes a member of the NeuroD family of basic-loop-helix transcription factors.  The NEUROD 1 was first described by Lee et al (1995) who observed that the gene was transiently expressed in subsets of neuronal cells of the central and peripheral nervous system at the time of their terminal differentiation into mature neurons. They also showed that ectopic expression of the NEUROD1 gene could convert non-neuronal cells to neuronal cells. The NEUROD1 protein forms heterodimers with other bHLH proteins and activates the transcription of a large set of genes that contain the E-box DNA sequence 5’-CANNTG’-3.  NEUROD1 is involved in the regulation of several cell differentiation pathways including the development of retinal ganglion cell, inner ear sensory neurons, and endocrine islet cells. NeuroD1 regulates the expression of numerous genes including the insulin gene. Mutations in the NEUROD1 gene has been associated with type II diabetes mellitus. Defects in expression of  NEUROD1 is linked with Maturity onset diabetes of the young type 6 (MODY6).  The NEUROD1 protein may be localized in the cytoplasm and the nucleus.  In Min 6 insulinoma cells Petersen et al (2002) were able to conclude that MEK-ERK associated phosphorylation of the serine 274 on NEUROD1 induces the translocation of the protein to the nucleus and that this may normally be associated with cell glucose concentration.

General readings

Peng,S.Y., et al., (2005) Biochim. Biophys. Acta 1731 (3), 154-159

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEUROD1 (N-term) antibody

Western blot analysis of hNeuroD1-Q30 (Cat. #TA324696) in HepG2 cell line lysates (35ug/lane). NEUROD1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu P
0.4 ml / €325.00
  OriGene Technologies, Inc.

NEUROD1 (201-300) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3H8 Purified Hu E, ICC/IF, P, WB

Regular Price: 50 µg / €530.00

Special Price: 50 µg / €294.00

  Acris Antibodies GmbH

NEUROD1 antibody

Immunoperoxidase of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml] Mouse IgG2a 3H8 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEUROD1 antibody

Western blot analysis of extracts from HeLa cells, using Neuro D (Ab-274) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NEUROD1 (C-term) antibody

NeuroD1 Antibody (I333) (Cat. #TA324697) western blot analysis in Y79 cell line ,mouse cerebellum and rat brain tissue lysates (35ug/lane).This demonstrates the NeuroD1 antibody detected the NeuroD1 protein (arrow). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NEUROD1 antibody

WB Suggested Anti-NEUROD1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEUROD1 antibody

WB Suggested Anti-NEUROD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Eq, Hu, Ms, Por, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

NEUROD1 antibody

Western blot analysis on HeLa cell lysate using Neuro D Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEUROD1 (201-300) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG 3D11 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

NEUROD1 antibody

Figure 1. Shows Neuro2A (Mouse neuroblastoma) and SKNSH, 1691 and IMR32 (Human neuroblastoma) cells stained with anti-NEUROD1 Antibody Cat.-No AM32710PU-N (Clone BC18-1.11EG) by the Indirect Immunofluorescence method. The secondary antibody was FITC conjugated anti-Mouse Ig. Nuclear and cytoplasmic staining for NEUROD is observed in all cells. Since the cell lines tested loosely attach to surfaces of chamber slides, the cells were immobilized on adhesion slides for the staining proceedure. The cells were allowed to attach to adhesion slides, fixed in 1% paraformaldehyde, permeabilized in 0.25% Triton X 100 in PBS, blocked with 1% BSA and stained overnight with 5 µg/ml antibody diluted with 1% BSA in PBS. The secondary antibody was diluted with 1% BSA in PBS according to manufacturers instruction and incubated for 2 hours. Mouse IgG1 BC18-1.11EG Purified Hu, Ms C, ICC/IF, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

NEUROD1 pSer274 antibody

Western blot analysis of extracts from HeLa cells treated with UV using Neuro D (Phospho-Ser274) Antibody AP55826PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

NEUROD1 pSer274 antibody

Western blot analysis of extracts from HeLa cells treated with UV using Neuro D (Phospho-Ser274) Antibody AP55826PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

NEUROD1 antibody

Western Blot analysis of NEUROD1 expression in transfected 293T cell line (H00004760-T01) by NEUROD1 MaxPab polyclonal antibody.

Lane 1: NEUROD1 transfected lysate(39.16 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

NEUROD1 antibody

Immunoprecipitation of NEUROD1 transfected lysate using anti-NEUROD1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NEUROD1 MaxPab mouse polyclonal antibody (B01) (H00004760-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

NEUROD1 antibody

Immunoperoxidase of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml] Mouse IgG 3D11 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEUROD1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with NEUROD1 polyclonal antibody ( Cat # PAB3857 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma Rabbit Hu E, P
0.1 mg / €390.00
  Abnova Taiwan Corp.

NEUROD1 antibody

Western blot analysis of NEUROD1 polyclonal antibody ( Cat # PAB3858 ) in mouse lung tissue lysate. NEUROD1 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

NEUROD1 pSer274 antibody

Western blot analysis of extracts from HeLa cells treated with UV 15', using Neuro D (Phospho-Ser274) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

NEUROD1 pSer274 antibody

Western blot analysis of extracts from HeLa cells treated with UV 15', using Neuro D (Phospho-Ser274) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

NEUROD1 antibody

Human Jurkat; WB Suggested Anti-NEUROD1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; NEUROD1 antibody - N-terminal region (ARP32036_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

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