Neurofilament H (200 kD) antibody

Principal name

Neurofilament H (200 kD) antibody

Alternative names for Neurofilament H (200 kD) antibody

NEFH,NF-H,KIAA0845, NFH, 200 kDa neurofilament protein, Neurofilament triplet H protein, (Neuronal Marker), heavy polypeptide

SwissProt ID

P12036 (Human), P12037 (Pig), P16884 (Rat), P19246 (Mouse)

Gene ID

4744 (NEFH), 24587 (Nefh), 380684

Available reactivities

All Species, Bov (Bovine), Can (Canine), Chk (Chicken), GP (Guinea Pig), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Sh (Sheep), Xen (Xenopus), Prim (Primate), Fe (Feline), Av (Birds), Por (Porcine), Hst (Hamster), Ze (Zebrafish), Mam (Mammalia)

Available hosts

Mouse, Chicken, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (capture) (E(capture)), Flow Cytometry (F)

Background of Neurofilament H (200 kD) antibody

Neurofilaments (NF) are ~10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and regulate axon diameter. Neurofilaments belong to the same protein family as the intermediate filaments of other tissues such as keratins, which make the filaments expressed in epithelia. The family of proteins that includes the intermediate filaments is divided into 5 major classes, the keratins forming the classes I and II. Class III contains the proteins vimentin, desmin, peripherin and glial fibrillary acidic protein (GFAP). The major neurofilament subunits occupy the class IV family of intermediate filaments, along with two other filament proteins of neurons, alpha-internexin and nestin. The class IV intermediate filament genes share two unique introns not found in other intermediate filament gene sequences. Finally, class V corresponds to intermediate filaments of the nuclear cytoskeleton, the nuclear lamins. Neurofibrils are bundles of neurofilaments. There are three major neurofilament subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE: The light or lowest (NF-L) runs at 68-70 kD. The medium or middle (NF-M) runs at about 145-160 kD. The heavy or highest (NF-H) runs at 200-220 kD. These three proteins are referred to as the "neurofilament triplet rdquo;. Antibodies against neurofilaments are useful for identification of neurons and their processes in histological sections and in tissue culture. The true molecular masses of these proteins are considerably lower than estimated based on SDS-PAGE mobility, particularly in the case of NF-H and NF-M. This is due to the highly charged C-terminal regions of the molecules. All three triplet proteins contain long stretches of polypeptide sequence rich in glutamic acid residues, and NF-M and especially NF-H also contain multiple tandemly repeated serine phosphorylation sites. These sites contain the peptide sequence lysine-serine-proline, and phosphorylation is predominantly found on axonal and not dendritic neurofilaments. Human NF-M has 13 of these KSP sites, while human NF-H is expressed from two alleles one of which produces 44 and the other 45 KSP repeats. The fourth class IV subunit, alpha-internexin (NF66) was discovered much later than NF-L, NF-M and NF-H, and is found co-polymerized with these proteins in most mature neurons. The fifth protein belonging to class IV, Nestin, is found in developing neurons and glia, and the presence of this protein is widely used to define neurogenesis. This protein is lost as development proceeds. The class III intermediate filament protein subunit peripherin is found in neurofilaments along with the class IV subunits in a few neurons, mostly in the peripheral nervous system. Finally another class III intermediate filament subunit, vimentin, is found in developing neurons and a fe

General readings

Hacker, G.W., et al., 1985, Histochemistry 82, 581-593.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Neurofilament H (200 kD) antibody

Western blot analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-05. Mouse IgG1 NF-05 Purified Hu, Por, Rt C, E, E(capture), WB
0.1 mg / €240.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-Neurofilament heavy protein (Clone NF-01). Mouse IgG1 NF-01 Purified All Species C, ICC/IF, P, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody [TA336619] - Western blot analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract.  The first lane is Coomassie Brilliant Blue stained and the second lane is probed with chicken anti-N Chicken IgY Purified Bov, Fe, Hu, Ms, Por, Rt C, ICC/IF, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Formalin-Fixed, Paraffin-Embedded Human cerebellum stained with Neurofilament Antibody Cat.-No AM32860PU (Clone NF421). Mouse IgG1 NF421 Purified Chk, Hu, Ms, Por, Rt C, E, F, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-Fixed, Paraffin-Embedded Human cerebellum stained with Neurofilament Antibody Cat.-No AM32860PU (Clone NF421). Mouse IgG1 NF421 Purified Chk, Hu, Ms, Por, Rt C, E, F, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-Fixed, Paraffin-Embedded Human cerebellum stained with Neurofilament Antibody Cat.-No AM32860PU (Clone NF421). Mouse IgG1 NF421 Purified Chk, Hu, Ms, Por, Rt C, E, F, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-Paraffin Cerebellum stained with Neurofilament Antibody Cat.-No AM33176PU (Clone RT-97). Mouse IgG1 RT97 Purified Hu, Ms, Por, Rt C, F, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-Paraffin Cerebellum stained with Neurofilament Antibody Cat.-No AM33176PU (Clone RT-97). Mouse IgG1 RT97 Purified Chk, Hu, Ms, Por, Rt C, F, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-Paraffin Cerebellum stained with Neurofilament Antibody Cat.-No AM33176PU (Clone RT-97). Mouse IgG1 RT97 Purified Hu, Ms, Por, Rt C, F, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-paraffin human cerebellum stained with Neurofilament Antibody Cat.-No AM50147PU (Clone SPM563). Mouse IgG1 SPM563 Purified Chk, Hu, Ms, Por, Rt C, F, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-paraffin human cerebellum stained with Neurofilament Antibody Cat.-No AM50147PU (Clone SPM563). Mouse IgG1 SPM563 Purified Chk, Hu, Ms, Por, Rt C, F, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-paraffin human cerebellum stained with Neurofilament Antibody Cat.-No AM50147PU (Clone SPM563). Mouse IgG1 SPM563 Purified Chk, Hu, Ms, Por, Rt C, F, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Western blot of rat cortex lysate showing specific immunolableing of the ~ 200k NF-H protein. Mouse IgG1 NAP4 Ascites Chk, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western blot of rat cortex lysate showing specific immunolableing of the ~200k NF-H protein. Chicken Ig Ig Fraction Chk, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Immunohistochemistry staining of Paraffin Brain tissue by Neurofilament antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic) Mouse IgG1 2F11 Purified Hu P
1 ml / €410.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody [TA336605] - Western blot analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract.  The first lane is Coomassie Brilliant Blue stained and the second lane is probed with rabbit anti-Ne Rabbit Purified Bov, Fe, Hu, Ms, Por, Rt C, E, ICC/IF, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody (NAP4) [TA336606] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibod Mouse IgG1 P4 Purified Hu, Mam, Ms, Rt C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody (AH1) [TA336709] - Strip blots of crude rat spinal cord extract stained with three different antibodies to phosphorylated NF-H, NB300-136 (lane 1), TA336709 (Lane 2) and NBP1-05210 (lane 3). All three anti Mouse IgG1 AH1 Purified Bov, Chk, Hu, Mam, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody (9B12) [TA336710] - Strip blots of crude rat spinal cord extract stained with three different antibodies to phosphorylated NF-H, NB300-136 (lane 1), NBP1-05209 (Lane 2) and TA336710 (lane 3). All three ant Mouse IgG2b 9B12 Purified Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody [TA336711] - Blot of rat spinal cord extract blotted with TA336711. A major band running at 200kDa is NF-H, the major neurofilament subunit protein. A minor band at about 160kDa is the non-phosphorylated d Rabbit Purified Chk, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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