Neurofilament H (200 kD) antibody

Principal name

Neurofilament H (200 kD) antibody

Alternative names for Neurofilament H (200 kD) antibody

NEFH,NF-H,KIAA0845, NFH, 200 kDa neurofilament protein, Neurofilament triplet H protein, (Neuronal Marker), heavy polypeptide

SwissProt ID

P12036 (Human), P12037 (Pig), P16884 (Rat), P19246 (Mouse)

Gene ID

4744 (NEFH), 4747 (NEFL), 24587 (Nefh)

Available reactivities

All Species, Bov (Bovine), Can (Canine), Chk (Chicken), GP (Guinea Pig), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Sh (Sheep), Xen (Xenopus), Prim (Primate), Fe (Feline), Por (Porcine), Av (Birds), Mam (Mammalia), Hst (Hamster), Ze (Zebrafish)

Available hosts

Mouse, Chicken, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (capture) (E(capture)), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Neurofilament H (200 kD) antibody

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia. This antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32 enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, this antibody labels a neuronal population with enhanced vulnerability to kainate toxicity, most of which are GABAergic and reveal kainate activated Ca2+ uptake.

General readings

The characterization of this antibody has not been formally published.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Neurofilament H (200 kD) antibody

Western blot analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-05. Mouse IgG1 NF-05 Purified Hu, Por, Rt C, E, E(capture), WB
0.1 mg / €240.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-Neurofilament heavy protein (Clone NF-01). Mouse IgG1 NF-01 Purified All Species C, ICC/IF, P, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody [TA336619] - Western blot analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract.  The first lane is Coomassie Brilliant Blue stained and the second lane is probed with chicken anti-N Chicken IgY Purified Bov, Fe, Hu, Ms, Por, Rt C, ICC/IF, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Formalin-Fixed, Paraffin-Embedded Human cerebellum stained with Neurofilament Antibody Cat.-No AM32860PU (Clone NF421). Mouse IgG1 NF421 Purified Chk, Hu, Ms, Por, Rt C, E, F, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-Fixed, Paraffin-Embedded Human cerebellum stained with Neurofilament Antibody Cat.-No AM32860PU (Clone NF421). Mouse IgG1 NF421 Purified Chk, Hu, Ms, Por, Rt C, E, F, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-Fixed, Paraffin-Embedded Human cerebellum stained with Neurofilament Antibody Cat.-No AM32860PU (Clone NF421). Mouse IgG1 NF421 Purified Chk, Hu, Ms, Por, Rt C, E, F, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-Paraffin Cerebellum stained with Neurofilament Antibody Cat.-No AM33176PU (Clone RT-97). Mouse IgG1 RT97 Purified Hu, Ms, Por, Rt C, F, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-Paraffin Cerebellum stained with Neurofilament Antibody Cat.-No AM33176PU (Clone RT-97). Mouse IgG1 RT97 Purified Chk, Hu, Ms, Por, Rt C, F, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-Paraffin Cerebellum stained with Neurofilament Antibody Cat.-No AM33176PU (Clone RT-97). Mouse IgG1 RT97 Purified Hu, Ms, Por, Rt C, F, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-paraffin human cerebellum stained with Neurofilament Antibody Cat.-No AM50147PU (Clone SPM563). Mouse IgG1 SPM563 Purified Chk, Hu, Ms, Por, Rt C, F, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-paraffin human cerebellum stained with Neurofilament Antibody Cat.-No AM50147PU (Clone SPM563). Mouse IgG1 SPM563 Purified Chk, Hu, Ms, Por, Rt C, F, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Formalin-paraffin human cerebellum stained with Neurofilament Antibody Cat.-No AM50147PU (Clone SPM563). Mouse IgG1 SPM563 Purified Chk, Hu, Ms, Por, Rt C, F, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Neurofilament H (200 kD) antibody

Western blot of rat cortex lysate showing specific immunolableing of the ~ 200k NF-H protein. Mouse IgG1 NAP4 Ascites Chk, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western blot of rat cortex lysate showing specific immunolableing of the ~200k NF-H protein. Chicken Ig Ig Fraction Chk, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Immunohistochemistry staining of Paraffin Brain tissue by Neurofilament antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic) Mouse IgG1 2F11 Purified Hu P
1 ml / €410.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody [TA336605] - Western blot analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract.  The first lane is Coomassie Brilliant Blue stained and the second lane is probed with rabbit anti-Ne Rabbit Purified Bov, Fe, Hu, Ms, Por, Rt C, E, ICC/IF, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody (NAP4) [TA336606] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibod Mouse IgG1 P4 Purified Hu, Mam, Ms, Rt C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody (AH1) [TA336709] - Strip blots of crude rat spinal cord extract stained with three different antibodies to phosphorylated NF-H, NB300-136 (lane 1), TA336709 (Lane 2) and NBP1-05210 (lane 3). All three anti Mouse IgG1 AH1 Purified Bov, Chk, Hu, Mam, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody (9B12) [TA336710] - Strip blots of crude rat spinal cord extract stained with three different antibodies to phosphorylated NF-H, NB300-136 (lane 1), NBP1-05209 (Lane 2) and TA336710 (lane 3). All three ant Mouse IgG2b 9B12 Purified Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament H (200 kD) antibody

Western Blot: 200kDa Neurofilament Heavy Antibody [TA336711] - Blot of rat spinal cord extract blotted with TA336711. A major band running at 200kDa is NF-H, the major neurofilament subunit protein. A minor band at about 160kDa is the non-phosphorylated d Rabbit Purified Chk, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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