Neurofilament L (68kDa) antibody

Principal name

Neurofilament L (68kDa) antibody

Alternative names for Neurofilament L (68kDa) antibody

NF-L,NEFL, NF68, 68 kDa neurofilament protein, Neurofilament L

SwissProt ID

P02547 (Pig), P02548 (Bovin), P07196 (Human), P08551 (Mouse), P19527 (Rat), P35616 (Xenla), Q02916 (Cotja)

Gene ID

4747 (NEFL), 18039 (Nefl), 281348, 83613

Available reactivities

Fe (Feline), Hu (Human), Ms (Mouse), Op (Opossum), Rb (Rabbit), Rt (Rat), Chk (Chicken), Bov (Bovine), Hst (Hamster), Mky (Monkey), Av (Birds), Por (Porcine), Mam (Mammalia)

Available hosts

Mouse, Chicken, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Neurofilament L (68kDa) antibody

Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation

General readings

Dahl D, Crosby CJ, Gardner EE and Bignami A: Purification of the glial fibrillary acidic protein by anion-exchange chromatography. Analyt Biochem 126, 165 ff (1982)
Weber K, Shaw G, Osborn M, Debus E and Geisler N: Neurofilaments, a subclass of intermediate filaments: Structure and expression. Cold Spring Harbor Symp Quant Biol 48, 717 ff (1983)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Neurofilament L (68kDa) antibody

  NEFL mouse monoclonal antibody, clone OTIG2 (formerly G2)  
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NEFL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801110) Mouse IgG1 OTIG2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament L (68kDa) antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining. Mouse IgG1 1H3 Purified Hu E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining. Mouse IgG1 2G10 Purified Hu E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

  Mouse Anti-Neurofilament NF-L ms Antibody  
Western blot of rat cortex lysate showing specific immunolableing of the ~ 68k NF-L protein. Mouse IgG1 DA2 Supernatant Chk, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

  Chicken Anti-Neurofilament NF-L ck Antibody  
Western blot of rat cortex lysate showing specific immunolableing of the ~ 68k NF-L protein. Chicken Ig Ig Fraction Bov, Chk, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

  Anti-NEFL Rabbit Polyclonal Antibody  
Predicted cell location: Cell membrane. Positive control: Human Colon and esophagus cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human Colon and esophagus cancer tissue using NEFL antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

  Rabbit Polyclonal NF-L Antibody  
Western Blot: 68kDa Neurofilament Light Antibody [TA336601] - Western blot of whole rat cerebellum homogenate stained with TA336601, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rode Rabbit Purified Bov, Hu, Mam, Ms, Por, Rt C, ICC/IF, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

  Mouse Monoclonal NF-L Antibody (DA2)  
Western Blot: 68kDa Neurofilament Light Antibody (DA2) [TA336602] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodie Mouse IgG1 DA2 Purified Hu, Mam, Ms, Rt C, ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

  Mouse Monoclonal NF-L Antibody (7D1)  
Western Blot: 68kDa Neurofilament Light Antibody (7D1) [TA336712] - Strip blots of rat spinal cord homogenate probed with two sublones of TA336712. Both subclones, one of which became the definitive antibody, stain strongly and specifically a band at 68kD Mouse IgG2b 7D1 Purified Bov, Hu, Mam, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

  Chicken Polyclonal NF-L Antibody  
Western Blot: 68kDa Neurofilament Light Antibody [TA336713] - Western blots of whole protein extracts of rat spinal cord (Lanes 1), brain stem (Lanes 2), cerebellum (Lanes 3) and cerebral cortex (Lanes 4). Neurofilaments are concentrated in large projecti Chicken IgY Purified Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

  Mouse Monoclonal Neurofilament NF-L Antibody (DA2)  
Western Blot: Neurofilament NF-L Antibody (DA2) Mouse DA2 Purified Bov, Chk, Fe, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

  NEFL mouse monoclonal antibody, clone OTI11F6 (formerly 11F6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFL (RC205920, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFL. Mouse IgG1 OTI11F6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament L (68kDa) antibody

  NEFL mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFL (RC205920, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFL. Mouse IgG2b OTI2F5 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament L (68kDa) antibody

Neurofilament L (68kDa) Mouse IgG1 NF-68 Purified Hu, Por, Rt C, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Immunohistochemistry analysis of NF-L in human lung carcinoma tissue (Formalin-fixed, Paraffin-embedded) using Neurofilament L antibody (Cat.-No. AP20786PU-N). Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Tissue section through an adult Mouse brain showing NFL (brown staining) of axons in the white matter of the cerebellum. Chicken Ig Aff - Purified Hu, Ms ICC/IF, P, WB
0.3 mg / €500.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Neurofilament L (68kDa) Mouse IgG LB-961 Ascites Bov, Hst, Hu, Mky, Ms, Rt C, ICC/IF, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) (+ Neurofilament H) antibody

Neurofilament L (68kDa) Mouse IgG1 2F11 Purified Fe, Hu, Ms, Op, Rb, Rt C, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) (+ Neurofilament H) antibody

Neurofilament L (68kDa) Mouse IgG1 2F11 Supernatant Hu C, P, WB
1 ml / €260.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) (+ Neurofilament H) antibody

Formalin-Fixed, Paraffin-Embedded Human cerebellum  stained with Neurofilament antibody Cat.-No. DM080X using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of neurons and neuronal processes. Mouse IgG1 2F11 Supernatant Fe, Hu, Ms, Op, Rb, Rt C, P, WB
1 ml / €340.00
  Acris Antibodies GmbH

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