Neurofilament L (68kDa) antibody

Principal name

Neurofilament L (68kDa) antibody

Alternative names for Neurofilament L (68kDa) antibody

NF-L,NEFL, NF68, 68 kDa neurofilament protein, Neurofilament L

SwissProt ID

P02547 (Pig), P02548 (Bovin), P07196 (Human), P08551 (Mouse), P19527 (Rat), P35616 (Xenla), Q02916 (Cotja)

Gene ID

4747 (NEFL), 18039 (Nefl), 733642 (LOC733642), 83613, 281348

Available reactivities

Fe (Feline), Hu (Human), Ms (Mouse), Op (Opossum), Rb (Rabbit), Rt (Rat), Chk (Chicken), Av (Birds), Bov (Bovine), Mam (Mammalia), Por (Porcine), Hst (Hamster), Mky (Monkey), Can (Canine)

Available hosts

Mouse, Chicken, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of Neurofilament L (68kDa) antibody

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria.

General readings

Dahl D, Crosby CJ, Gardner EE and Bignami A: Purification of the glial fibrillary acidic protein by anion-exchange chromatography. Analyt Biochem 126, 165 ff (1982)
Weber K, Shaw G, Osborn M, Debus E and Geisler N: Neurofilaments, a subclass of intermediate filaments: Structure and expression. Cold Spring Harbor Symp Quant Biol 48, 717 ff (1983)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Neurofilament L (68kDa) antibody

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NEFL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801110) Mouse IgG1 OTIG2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament L (68kDa) antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining. Mouse IgG1 1H3 Purified Hu E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining. Mouse IgG1 2G10 Purified Hu E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Western blot of rat cortex lysate showing specific immunolableing of the ~ 68k NF-L protein. Mouse IgG1 DA2 Supernatant Chk, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

Western blot of rat cortex lysate showing specific immunolableing of the ~ 68k NF-L protein. Chicken Ig Ig Fraction Bov, Chk, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

Predicted cell location: Cell membrane. Positive control: Human Colon and esophagus cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human Colon and esophagus cancer tissue using NEFL antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

Western Blot: 68kDa Neurofilament Light Antibody [TA336601] - Western blot of whole rat cerebellum homogenate stained with TA336601, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rode Rabbit Purified Bov, Hu, Mam, Ms, Por, Rt C, ICC/IF, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

Western Blot: 68kDa Neurofilament Light Antibody (DA2) [TA336602] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodie Mouse IgG1 DA2 Purified Hu, Mam, Ms, Rt C, ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

Western Blot: 68kDa Neurofilament Light Antibody (7D1) [TA336712] - Strip blots of rat spinal cord homogenate probed with two sublones of TA336712. Both subclones, one of which became the definitive antibody, stain strongly and specifically a band at 68kD Mouse IgG2b 7D1 Purified Bov, Hu, Mam, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

Western Blot: 68kDa Neurofilament Light Antibody [TA336713] - Western blots of whole protein extracts of rat spinal cord (Lanes 1), brain stem (Lanes 2), cerebellum (Lanes 3) and cerebral cortex (Lanes 4). Neurofilaments are concentrated in large projecti Chicken IgY Purified Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

Western Blot: Neurofilament NF-L Antibody (DA2) Mouse DA2 Purified Bov, Chk, Fe, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament L (68kDa) antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFL (RC205920, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFL. Mouse IgG1 OTI11F6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament L (68kDa) antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFL (RC205920, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFL. Mouse IgG2b OTI2F5 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament L (68kDa) antibody

Neurofilament L (68kDa) Mouse IgG 7D1 Mam ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Neurofilament L (68kDa) Mouse IgG1 NF-68 Purified Hu, Por, Rt C, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Neurofilament L (68kDa) Rabbit IgG Liquid Chk, Mam C, ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Neurofilament L (68kDa) Chicken Ig Liquid Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Immunohistochemistry analysis of NF-L in human lung carcinoma tissue (Formalin-fixed, Paraffin-embedded) using Neurofilament L antibody (Cat.-No. AP20786PU-N). Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Tissue section through an adult Mouse brain showing NFL (brown staining) of axons in the white matter of the cerebellum. Chicken Ig Aff - Purified Hu, Ms ICC/IF, P, WB
0.3 mg / €450.00
  Acris Antibodies GmbH

Neurofilament L (68kDa) antibody

Neurofilament L (68kDa) Mouse IgG LB-961 Ascites Bov, Hst, Hu, Mky, Ms, Rt C, ICC/IF, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

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