Neurofilament M (160 kD) antibody

Principal name

Neurofilament M (160 kD) antibody

Alternative names for Neurofilament M (160 kD) antibody

Neurofilament medium polypeptide, NF-M, NEF3, NEFM, Neurofilament 3, (Neuronal Marker)

SwissProt ID

O77788 (Bovin), P07197 (Human), P08552 (Pig), P08553 (Mouse), P12839 (Rat), P16053 (Chick), P54938 (Rabit)

Gene ID

4741 (NEFM), 24588 (Nefm), 18040

Available reactivities

All Species, Hst (Hamster), Hu (Human), Mky (Monkey), Rt (Rat), Xen (Xenopus), GP (Guinea Pig), Rb (Rabbit), Chk (Chicken), Fe (Feline), Ms (Mouse), Bov (Bovine), Av (Birds), Por (Porcine), Mam (Mammalia), Fi (Fish), Can (Canine), Eq (Equine), Sh (Sheep)

Available hosts

Mouse, Chicken, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E)

Background of Neurofilament M (160 kD) antibody

Neurol intermediate filaments (NIF) are type-IV intermediate filaments found specifically on neurons where they play key role in maintaining neurol morphology as well as in regenerating myelited axons. NIF contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. Mature NIFs are heteropolymers with about 4:2:1 stoichiometric ratio of NEFL: NEFM: NEFH and also contain a fourth type-IV intermediate filament (IF) subunit, alpha-internexin. NEFM is essential to cross-bridge formation, stabilization, and longitudil extension of filamentous network and is more critical than NEFH in regulating NIF's structure and function. NEFM is crucial for the acquisition of normal axol caliber in response to a myelin-dependent "outside-in" trigger for radial axol growth and removal of the tail domain as well as lysine-serine-proline (KSP) repeats of NEFM, but not NEFH, produces axons with impaired radial growth and reduced conduction velocities. Moreover, mutations of NEFM have been associated with CNS/PNS disorders such as ALS, Parkinson's disease etc., and an imbalance in NEFM's O-Glccylation - phosphorylation regulation plays a crucial role in in Alzheimer disease.

General readings

1. Ohara O, Gahara Y, Miyake T, Teraoka H, Kitamura T.: Neurofilament deficiency in quail caused by nonsense mutation in neurofilament-L gene. J Cell Biol. 1993 Apr;121(2):387-95.
2. Rao MV, Campbell J, Yuan A, Kumar A, Gotow T, Uchiyama Y, Nixon RA: The neurofilament middle molecular mass subunit carboxyl-terminal tail domains is essential for the radial growth and cytoskeletal architecture of axons but not for regulating neurofilament transport rate. J Cell Biol. 2003 Dec 8;163(5):1021-31.
3. Deng Y, Li B, Liu F, Iqbal K, Grundke-Iqbal I, Brandt R, Gong CX: Regulation between O-GlcNAcylation and phosphorylation of neurofilament-M and their dysregulation in Alzheimer disease. FASEB J. 2008 Jan;22(1):138-45.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Neurofilament M (160 kD) antibody

  NEFM mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)  
Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805823) Mouse IgG1 OTI2G3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament M (160 kD) antibody

  NEFM mouse monoclonal antibody, clone OTI5B7 (formerly 5B7)  
Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805948) Mouse IgG1 OTI5B7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament M (160 kD) antibody

Neurofilament M Antibody (AP09628PU-N, 0.03µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Neurofilament M (160 kD) antibody

  Mouse Anti-Neurofilament NF-M ms Antibody  
Western blot of rat cortex lysate showing specific immunolableing of the ~ 145k NF-M protein. Mouse IgG1 3H11 Supernatant Chk, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament M (160 kD) (C-term) antibody

  Chicken Anti-Neurofilament NF-M ck Antibody  
Western blot of rat cortex lysate showing specific immunolableing of the ~ 145k NF-M protein. Chicken Ig Ig Fraction Chk, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament M (160 kD) antibody

  Rabbit Polyclonal Anti-NEFM Antibody  
WB Suggested Anti-NEFM Antibody; Titration: 1.0 ug/ml; Positive Control: ACHN Whole Cell Rabbit IgG Purified Bov, Can, Eq, Hu, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

Neurofilament M (160 kD) antibody

  Rabbit Polyclonal Anti-Nefm Antibody  
Host: Rabbit; Target Name: Nefm; Sample Tissue: Rat Muscle lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

Neurofilament M (160 kD) antibody

  Rabbit Polyclonal NF-M Antibody  
Western Blot: 160kDa Neurofilament Medium Antibody [TA336603] - Western blot analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using TA336603. Rabbit Purified Bov, Fe, Hu, Ms, Por, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament M (160 kD) antibody

  Mouse Monoclonal NF-M Antibody (3H11)  
Western Blot: 160kDa Neurofilament Medium Antibody (3H11) [TA336604] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibo Mouse IgG1 3H11 Purified Hu, Mam, Ms, Rt C, ICC/IF, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Neurofilament M (160 kD) antibody

  Chicken Polyclonal NF-M Antibody  
Western Blot: 160kDa Neurofilament Medium Antibody [TA336622] - Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with TA336622, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates Chicken IgY Purified Chk, Fe, Fi, Hu, Ms, Rt ICC/IF, WB
0.25 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament M (160 kD) antibody

  Rabbit Polyclonal Anti-NEFM Antibody  
Neurofilament  M (160 kD) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neurofilament M (160 kD) antibody

  NEFM mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)  
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEFM(RC224475) using Anti-NEFM mouse monoclonal antibody (TA506753) at 1:100 dilution. Mouse IgG1 OTI1A4 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament M (160 kD) antibody

  NEFM mouse monoclonal antibody, clone OTI2C4 (formerly 2C4)  
Anti-NEFM mouse monoclonal antibody (TA506794) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEFM(RC224475). Mouse IgG1 OTI2C4 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament M (160 kD) antibody

  NEFM mouse monoclonal antibody, clone OTI5H4 (formerly 5H4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (RC224475, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM. Mouse IgG1 OTI5H4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Neurofilament M (160 kD) antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG2a NF-09 Aff - Purified Por ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Neurofilament M (160 kD) antibody

Neurofilament  M (160 kD) Mouse IgG1 403 Supernatant Hu C, P
1 ml / €390.00
  Acris Antibodies GmbH

Neurofilament M (160 kD) antibody

Western blot (WB) analyzes of NF-M antibody (Cat.-No.: AP20680PU-N) in extracts from MCF-7cells. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Neurofilament M (160 kD) (C-term) antibody

Dissociated cell culture prepared from an adult Rat brain. NFM (green staining) stains neurites in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to costain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining. Picture courtesy of Dr. Gerry Shaw, University of Florida. Chicken Ig Aff - Purified Rt ICC/IF, P, WB
0.2 ml / €340.00
  Acris Antibodies GmbH

Neurofilament M (160 kD) antibody

Neurofilament  M (160 kD) Mouse IgG1 NF403 Supernatant Bov, GP, Hu, Rt C, P, WB
1 ml / €330.00
  Acris Antibodies GmbH

Neurofilament M (160 kD) antibody

Immunofluorescence staining of Rat brain Frozen Sections (Acetone fixed) with a 1/100 dilution of BM6011P (Clone RNF403) directed against the 160kD component of neurofilaments. Mouse IgG1 RNF403 Purified Hst, Hu, Mky, Rt, Xen C, ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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