Neuron specific enolase antibody

Principal name

Neuron specific enolase antibody

Alternative names for Neuron specific enolase antibody

NSE, ENO2, Enolase 2,Neural enolase, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase

SwissProt ID

O57391 (Chick), P07323 (Rat), P09104 (Human), P17183 (Mouse)

Gene ID

2026 (ENO2), 13807 (Eno2), 24334

Available reactivities

Hu (Human), GP (Guinea Pig), Ms (Mouse), Can (Canine), Mky (Monkey), Prim (Primate), Rt (Rat), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Chicken, Sheep

Available applications

Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Neuron specific enolase antibody

Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. (Ref.2)

General readings

1. Sun Y  et al. The expression and significance of neuronal iconic proteins in podocytes. PLoS One 9:e93999 (2014). WB, IHC-P, ICC/IF ; Mouse, Human .
2. Ren Y  et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). ICC/IF ; Goat .
3. Ding J & Delpire E Deletion of KCC3 in parvalbumin neurons leads to locomotor deficit in a conditional mouse model of peripheral neuropathy associated with agenesis of the corpus callosum. Behav Brain Res 274:128-36 (2014). Mouse .
4. Kovesdi E  et al. Acute minocycline treatment mitigates the symptoms of mild blast-induced traumatic brain injury. Front Neurol 3:111 (2012). Rat .
5. Kwon SK  et al. Stress and traumatic brain injury: a behavioral, proteomics, and histological study. Front Neurol 2:12 (2011). Dot Blot ; Rat .
6. Gyorgy A  et al. Time-dependent changes in serum biomarker levels after blast traumatic brain injury. J Neurotrauma 28:1121-6 (2011). WB, HPLC, IP ; Pig .
7. Qi J  et al. Siah2-Dependent Concerted Activity of HIF and FoxA2 Regulates Formation of Neuroendocrine Phenotype and Neuroendocrine Prostate Tumors. Cancer Cell 18:23-38 (2010). IHC ; Human .
8. Filiano AJ  et al. Transglutaminase 2 protects against ischemic stroke. Neurobiol Dis 39:334-43 (2010). IHC-P ; Human .
9. Yu Y  et al. Evaluation of blastomere biopsy using mouse model indicates the potential high-risk of neurodegenerative disorders in the offspring. Mol Cell Proteomics : (2009). WB, IHC-FoFr ; Mouse .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Neuron specific enolase antibody

  Rabbit polyclonal anti-NSE / ENO2 antibody  
Western blot analysis of extracts from HepG2 cells, using NSE antibody (#TA311937).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neuron specific enolase antibody

  Rabbit Polyclonal Enolase 2/Neuron-specific Enolase Antibody  
Western Blot: NSE Antibody [TA336531] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa. Rabbit Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neuron specific enolase antibody

Western Blot analysis of ENO2 expression in transfected 293T cell line by ENO2 monoclonal antibody (M01), clone 1A3.

Lane 1: ENO2 transfected lysate(47.3 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1A3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Neuron specific enolase antibody

  Rabbit Anti-NSE (Neuron specific enolase) Antibody  
Western blot of rat cortex homogenate showing specific immunolabeling of the ~ 47k NSE protein. Rabbit IgG Serum Hu, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neuron specific enolase antibody

  Anti-ENO2 Rabbit Polyclonal Antibody  
Predicted band size: 47 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neuron specific enolase antibody

  Rabbit anti-ENO2 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ENO2antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Neuron specific enolase antibody

  Rabbit Polyclonal Anti-NSE Antibody  
Western blot analysis on HepG2 cell lysate using NSE Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Neuron specific enolase antibody

  Mouse monoclonal Anti-NSE Clone NSEP1  
Western Blot of NSE protein standards using anti-NSE clone NSEP1 and NSEP2. Mouse IgG1 NSEP1 Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Neuron specific enolase antibody

  Mouse monoclonal Anti-NSE Clone NSEP2  
Western Blot of NSE protein standards using anti-NSE clone NSEP1 and NSEP2. Mouse IgG1 NSEP2 Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Neuron specific enolase antibody

  Rabbit Polyclonal anti-Neuron Specific Enolase (NSE) Antibody  
Human Pancreas stained with anti-Neuron Specific Enolase (NSE) antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neuron specific enolase antibody

  Rabbit Polyclonal NSE Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENO2 (RC201085, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENO2. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Neuron specific enolase antibody

Formalin-Fixed, Paraffin-Embedded Human pancreas stained with NSE antibody Cat.-No. AM00578SU-N using peroxidase conjugate and DAB chromogen. Note cytoplasmic staining of islet cells. Mouse IgG1 E27 Supernatant Hu P, WB
1 ml / €490.00
  Acris Antibodies GmbH

Neuron specific enolase antibody

Neuron specific enolase Mouse IgG2b 145 Aff - Purified Hu E, WB
0.5 mg / €420.00
  Acris Antibodies GmbH

Neuron specific enolase antibody

Neuron specific enolase Mouse IgM 1G6 Aff - Purified Hu E, WB
0.5 mg / €420.00
  Acris Antibodies GmbH

Neuron specific enolase antibody

Neuron specific enolase Mouse IgG1 171 Aff - Purified Hu E
0.5 mg / €420.00
  Acris Antibodies GmbH

Neuron specific enolase antibody

Neuron specific enolase Mouse IgG1 NSE-P2 Purified Hu C, E, WB
0.2 mg / €370.00
  Acris Antibodies GmbH

Neuron specific enolase antibody

The extracts of mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT17D10 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Neuron specific enolase antibody

The extracts of mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT17D10 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

Neuron specific enolase antibody

Immunohistochemistry analyzes of NSE/ENO2 antibody (Cat.-No AP06253PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Neuron specific enolase antibody

Western blot analysis of anti-NSE Antibody (Y236) (Cat.#AP12426PU-N) in Y79 cell line lysates (35ug/lane). NSE (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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