Neuronal acetylcholine receptor subunit alpha-3 antibody

Principal name

Neuronal acetylcholine receptor subunit alpha-3 antibody

Alternative names for Neuronal acetylcholine receptor subunit alpha-3 antibody

Nicotinic acetylcholine receptor subunit alpha 3, CHRNA3, NACHRA3

SwissProt ID

P04757 (Rat), P32297 (Human), Q07263 (Bovin), Q86U77 (Human), Q8R4G9 (Mouse)

Gene ID

1136 (CHRNA3), 25101 (Chrna3), 110834

Available reactivities

Can (Canine), Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E)

Background of Neuronal acetylcholine receptor subunit alpha-3 antibody

Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (ChRs) which belong to the ligand-gated ion channel superfamily. ChRs also respond to nicotine, hence their me.To date, 17 different but related subunits of ChRs have been identified and cloned. They consist of a subunits (a1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding. The other subunits responsible for making up the active receptor are the Ã? (Ã?1-4), ?, d and e subunits3. Structurally, all subunits have the following: a conserved large extracellular N-termil domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-termil domain. An active ChR is generally a heteropentamer of these various subunits organized around a central pore.All a subunits are expressed in neurol cells except for the a1 subunit which is specifically expressed in the muscle. In the peripheral autonomic nervous system, nicotinic receptors are prominent and are responsible mediating fast syptic transmission in all peripheral autonomic ganglia. In this system, these channel receptors are mostly made up of two a3 subunits heteromerized with three other subunits. The a3 subunit plays a most important role in this system as knockout mice suffer from severe autonomic failure accompanied by gastrointestil and bladder malfunctions.Autoimmune autonomic ganglionopathy (AAG) is a neurological disorder characterized by dysfunctions in sympathetic, parasympathetic and enteric functions. Patients suffering from the disease express high levels of self antibodies against ionotropic nicotinic receptors, mely a3 subunits4. The a3 subunit is also found in dorsal root ganglion where it is most probably involved in nociception.

General readings

Criado JR., et al. (2014) Twin Res Him Genet. 17(2):80-8.Rempel N., et al. (1998) Hum. Genet. 103:645-653.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Neuronal acetylcholine receptor subunit alpha-3 antibody

Predicted band size: 55 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-3 antibody

Western blot analysis of CHRNA3 Antibody (N-term) (Cat. #TA324798) in CEM cell line lysates (35ug/lane).CHRNA3 (arrow) was detected using the purified Pab. Rabbit IgG Purified Bov, Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-3 antibody

Western blot analysis of rat brain membranes: 1. Anti-Nicotinic Acetylcholine Receptor a3(extracellular) antibody, (1:200). 2. Anti-Nicotinic Acetylcholine Receptor a3 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-3 antibody

WB Suggested Anti-CHRNA3 Antibody Titration: 0.1ug/ml; Positive Control: Human Thymus Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-3 antibody

WB Suggested Anti-CHRNA3 Antibody Titration: 1.0ug/ml; ELISA Titer: 1:312500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-3 antibody

WB Suggested Anti-CHRNA3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-3 antibody

Neuronal acetylcholine receptor subunit alpha-3 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Neuronal acetylcholine receptor subunit alpha-3 antibody

Human COLO205; WB Suggested Anti-CHRNA3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: COLO205 cell lysate; CHRNA3 antibody - N-terminal region (ARP34964_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Neuronal acetylcholine receptor subunit alpha-3 antibody

Human Thymus; WB Suggested Anti-CHRNA3 Antibody Titration: 0.1ug/ml. Positive Control: Human Thymus; CHRNA3 antibody - N-terminal region (AVARP13014_P050) in Human Thymus cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

Neuronal acetylcholine receptor subunit alpha-3 antibody

Human MCF-7; WB Suggested Anti-CHRNA3 Antibody Titration: 1.0ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate; CHRNA3 antibody - N-terminal region (AVARP13024_T100) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Can, Hu P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

Neuronal acetylcholine receptor subunit alpha-3 antibody

Western blot analysis of CHRNA3 expression in A549 (A); HepG2 (B); mouse liver (C); rat kidney (D) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Neuronal acetylcholine receptor subunit alpha-3 antibody

Western blot analysis of CHRNA3 expression in A549 (A); HepG2 (B); mouse liver (C); rat kidney (D) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Neuronal acetylcholine receptor subunit alpha-3 antibody

Western blot analysis of CHRNA3 expression in A549 (A); HepG2 (B); mouse liver (C); rat kidney (D) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Neuronal acetylcholine receptor subunit alpha-3 (32-240, His-tag)

Neuronal acetylcholine receptor subunit alpha-3 Human Purified > 80 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Neuronal acetylcholine receptor subunit alpha-3 (32-240, His-tag)

Neuronal acetylcholine receptor subunit alpha-3 Human Purified > 80 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Neuronal acetylcholine receptor subunit alpha-3

Neuronal acetylcholine receptor subunit alpha-3 Human
2 µg / €300.00
  Abnova Taiwan Corp.

Neuronal acetylcholine receptor subunit alpha-3

Neuronal acetylcholine receptor subunit alpha-3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Neuronal acetylcholine receptor subunit alpha-3

Neuronal acetylcholine receptor subunit alpha-3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CHRNA3 (mGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, CHRNA3 (mGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, CHRNA3 (mGFP-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, CHRNA3 (mGFP-tagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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