Neuronal acetylcholine receptor subunit alpha-4 antibody

Principal name

Neuronal acetylcholine receptor subunit alpha-4 antibody

Alternative names for Neuronal acetylcholine receptor subunit alpha-4 antibody

Nicotinic acetylcholine receptor subunit alpha 4, CHRNA4, NACRA4

SwissProt ID

O70174 (Mouse), P09482 (Chick), P09483 (Rat), P43681 (Human)

Gene ID

1137 (CHRNA4), 25590 (Chrna4), 11438

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Neuronal acetylcholine receptor subunit alpha-4 antibody

Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (ChRs) which belong to the ligand-gated ion channel superfamily. ChRs also respond to nicotine, hence their me. These channel receptors are usually non-selective cation channels activated upon ligand binding which ultimately leads to the depolarization of postsyptic cell membranes. To date, 17 different but related subunits of ChRs have been identified and cloned. They consist of a subunits (a1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding. The other subunits responsible for making up the active receptor are the Ã? (Ã?1-4), ?, d and e subunits. Structurally, all subunits have the following: a conserved large extracellular N-termil domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-termil domain. An active ChR is generally a heteropentamer (homopentamers also exist) of these various subunits organized around a central pore. All a subunits are expressed in neurol cells except for the a1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neurol cells such as bronchial epithelial cells, as well lymphocytes. The diversity of these receptors and their functiol organization gives rise to unique properties and functions. The a4Ã?2 receptor composition makes up a high affinity nicotinic receptor. In fact, its upregulation (mainly expressed by the increase of functiol receptors at the membrane and not expression per se) is responsible for the increased appearance of binding sites following nicotine administration. Animal studies have shown that ChR-related mechanisms are involved in attention function. Indeed a4Ã?2 ChR seems to also be involved in attention-deficit hyperactivity disorder (ADHD), a disease distinguished by a lack of attention, distractibility and hyperactivity. The a4Ã?2 and a7 ChRs appear to be critical in rats for attention and working memory. Also, a a4Ã?2 specific agonist was shown to reduce impulsivity, hyperactivity and attention deficits in adults with ADHD. This same receptor subtype may also be involved in Parkinsonâ??s disease (PD) as smoking and a4Ã?2 ChR agonists show beneficial effects in PD.

General readings

Schwarz J, Schwarz SC, Dorigo O, Stützer A, Wegner F, Labarca C, Deshpande P, Gil JS, Berk AJ, Lester HA. Enhanced expression of hypersensitive alpha4* nAChR in adult mice increases the loss of midbrain dopaminergic neurons. FASEB J. 2006 May;20(7):935-46. PMID: 16675851

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Neuronal acetylcholine receptor subunit alpha-4 (29-43) antibody

TA302451 (0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-4 antibody

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates: 1, 2. Anti-Nicotinic Acetylcholine Receptor a4 (extracellular) antibody, (1:200). 3, 4. Anti-Nicotinic Acetylcholine Receptor a4 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-4 antibody

WB Suggested Anti-CHRNA4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-4 antibody

WB Suggested Anti-CHRNA4 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-4 antibody

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Lovo cells, 293T cells, Primary antibody: (CHRNA4 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-4 antibody

Neuronal acetylcholine receptor subunit alpha-4 Rat IgG1 299 Purified Chk, Hu, Ms, Rt ICC/IF, IP
500 µl / €379.00
  OriGene Technologies, Inc.

Neuronal acetylcholine receptor subunit alpha-4 antibody

Neuronal acetylcholine receptor subunit alpha-4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Neuronal acetylcholine receptor subunit alpha-4 antibody

Western Blot analysis of CHRNA4 expression in transfected 293T cell line (H00001137-T01) by CHRNA4 MaxPab polyclonal antibody.

Lane 1: CHRNA4 transfected lysate(49.61 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Neuronal acetylcholine receptor subunit alpha-4 antibody

Western Blot analysis of CHRNA4 expression in transfected 293T cell line (H00001137-T02) by CHRNA4 MaxPab polyclonal antibody.

Lane 1: CHRNA4 transfected lysate(49.61 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Neuronal acetylcholine receptor subunit alpha-4 antibody

Western Blot analysis of CHRNA4 expression in transfected 293T cell line (H00001137-T02) by CHRNA4 MaxPab polyclonal antibody.

Lane 1: CHRNA4 transfected lysate(49.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Neuronal acetylcholine receptor subunit alpha-4 antibody

Neuronal acetylcholine receptor subunit alpha-4 Mouse IgG1 4E3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Neuronal acetylcholine receptor subunit alpha-4 antibody

Neuronal acetylcholine receptor subunit alpha-4 Goat Hu E
0.1 mg / €390.00
  Abnova Taiwan Corp.

Neuronal acetylcholine receptor subunit alpha-4 antibody

Human HepG2; WB Suggested Anti-CHRNA4 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; CHRNA4 antibody - N-terminal region (ARP34965_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Neuronal acetylcholine receptor subunit alpha-4 antibody

Human HepG2; WB Suggested Anti-CHRNA4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; CHRNA4 antibody - N-terminal region (AVARP13015_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

Chrna4 - mouse gene knockout kit via CRISPR

  Chrna4 - mouse gene knockout kit via CRISPR  
Chrna4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Chrna4 - mouse gene knockout kit via CRISPR

  Chrna4 - mouse gene knockout kit via CRISPR  
Chrna4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Chrna4 - mouse gene knockout kit via CRISPR

  Chrna4 - mouse gene knockout kit via CRISPR  
Chrna4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Chrna4 - mouse gene knockout kit via CRISPR

  Chrna4 - mouse gene knockout kit via CRISPR  
Chrna4 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Neuronal acetylcholine receptor subunit alpha-4

Neuronal acetylcholine receptor subunit alpha-4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Neuronal acetylcholine receptor subunit alpha-4

Neuronal acetylcholine receptor subunit alpha-4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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