Neuroserpin / SERPINI1 antibody
Principal name
Neuroserpin / SERPINI1 antibody
Alternative names for Neuroserpin / SERPINI1 antibody
Serpin I1, PI12, PI-12, Peptidase inhibitor 12
Ncbi ID
9606, NP_001116224.1, NP_005016, NP_001116224, NM_009250, NM_053779, NM_005025, NM_001122752
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Neuroserpin / SERPINI1 antibody
Neuroserpin is an inhibitory serpin that is expressed predominantly in central nervous system. Although the physiological target of neuroserpin is still unclear, cumulative evidence suggest that it plays an important role in controlling proteolytic degradation of extracellular matrix (ECM) during synaptogenesis and the subsequent development of neuronal plasticity. In the adult brain, neuroserpin is secreted from the growth cones of neurons in areas where synaptic changes are associated with learning and memory, i.e. cerebral cortex, hippocampus, and amygdala. The neuroprotective role of neuroserpin has been demonstrated in transgenic mice lacking neuroserpin expression. The deficiency of neuroserpin in these mice was associated with motor neuron disease characterized by axonal degradation. In humans, defects in neuroserpin, caused by point mutations in the neuroserpin gene, underlie a hereditary disorder called the familial encephalopathy with neuroserpin inclusion bodies (FENIB).
General readings
Onda M, Belorgey D, Sharp LK, Lomas DA. Latent Ser49Pro neuroserpin forms polymers in the dementia familial encephalopathy with neuroserpin inclusion bodies. J Biol Chem. 2005 Jan 21
Immunoperoxidase of monoclonal antibody to SERPINI1 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml]
SERPINI1 monoclonal antibody (M01), clone 1D10 Western Blot analysis of SERPINI1 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged SERPINI1 is approximately 0.3ng/ml as a capture antibody.
SERPINI1 polyclonal antibody ( Cat # PAB6763 ) ( 1 µg/ml ) staining of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
SERPINI1 monoclonal antibody (M03), clone 1E10. Western Blot analysis of SERPINI1 expression in HeLa ( Cat # L013V1 ).
Western blot (WB) analysis of Neuropsin antibody (Cat.-No.: AP01329PU-N) in extracts from Jurkat cells.
Human NCI-H226; WB Suggested Anti-SERPINI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: NCI-H226 cell lysate; SERPINI1 antibody - middle region (ARP56616_P050) in Human NCI-H226 cells using Western Blot
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
SERPINI1 monoclonal antibody AM31816PU-N, Western Blot analysis of SERPINI1 expression in HeLa.
Detection limit for recombinant GST tagged SERPINI1 is approximately 0.3ng/ml as a capture antibody.
Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human brain tissue stained with SERPINI1 antibody (N-term) Cat.-No AP53865PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SERPINI1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal Immunofluorescent analysis of SERPINI1 Antibody (N-term)Cat.-No AP53865PU-N with HepG2 cell followed by Alexa Fluor® 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis using SERPINI1 Antibody (N-term) Cat.-No AP53865PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the SERPINI1 antibody detected the SERPINI1 protein (arrow).
TA303009 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted band size: 46 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
To detect Human Neuroserpin by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human Neuroserpin.
To detect hNeuroserpin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Neuroserpin (TA328402) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeuroserpin.
To detect hNeuroserpin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Neuroserpin (TA328402) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeuroserpin.
WB Suggested Anti-SERPINI1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: NCI-H226 cell lysate
Western blot analysis of extract from Mouse brain tissue using Neuroserpin Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA303009 | Neuroserpin / SERPINI1 antibody |
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| Goat Polyclonal Antibody against SERPINI1 | |||||||
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Goat | Aff - Purified | Chimp, Hu, Ms, Rt | WB |
0.1 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA323794 | Neuroserpin / SERPINI1 antibody |
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| Anti-SERPINI1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA328402 | Neuroserpin / SERPINI1 antibody |
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| Biotinylated Anti-Human Neuroserpin Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA328403 | Neuroserpin / SERPINI1 antibody |
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| Anti-Human Neuroserpin Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA342233 | Neuroserpin / SERPINI1 antibody |
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| Rabbit polyclonal Anti-SERPINI1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, Hu, Ms, Rb, Ze | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347830 | Neuroserpin / SERPINI1 antibody |
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| Rabbit Polyclonal Anti-Neuroserpin Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| AM31816PU-N | Neuroserpin / SERPINI1 antibody |
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 |
Mouse | IgG1 | 1D10 | Purified | Hu | E, P, WB |
50 µg /
€530.00
|
| Acris Antibodies GmbH | |||||||
| +2 additional images |
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| AP01329PU-N | Neuroserpin / SERPINI1 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 mg /
€350.00
|
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| Acris Antibodies GmbH | |||||||
| AP53865PU-N | Neuroserpin / SERPINI1 (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | ICC/IF, P, WB |
0.4 ml /
€370.00
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|
| Acris Antibodies GmbH | |||||||
| +2 additional images |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205290 | SERPINI1 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN205290BN | SERPINI1 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205290LP | SERPINI1 - human gene knockout kit via CRISPR |
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|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205290RB | SERPINI1 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP723330 | Neuroserpin / SERPINI1 (transcript variant 1) |
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| Purified recombinant protein of Human serpin peptidase inhibitor, clade I (neuroserpin), member 1 (SERPINI1), transcript variant 1. | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
Regular Price: 25 µg / €199.00 Special Price: 25 µg / €139.00 |
|
| OriGene Technologies, Inc. | |||||
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Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC205290L2V | Lenti ORF particles, SERPINI1 (mGFP-tagged) - Human serpin peptidase inhibitor, clade I (neuroserpin), member 1 (SERPINI1), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,050.00
|
|
| OriGene Technologies, Inc. | ||
| RC225640L2V | Lenti ORF particles, SERPINI1 (mGFP-tagged)-Human serpin peptidase inhibitor, clade I (neuroserpin), member 1 (SERPINI1), transcript variant 2, 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,050.00
|
|
| OriGene Technologies, Inc. | ||
| RC205290L1V | Lenti ORF particles, SERPINI1 (Myc-DDK tagged) - Human serpin peptidase inhibitor, clade I (neuroserpin), member 1 (SERPINI1), transcript variant 1 , 200 uL, >10^7 TU/mL |
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|
200 µl /
€940.00
|
|
| OriGene Technologies, Inc. | ||
| RC225640L1V | Lenti ORF particles, SERPINI1 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade I (neuroserpin), member 1 (SERPINI1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€940.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206454L2V | Lenti ORF particles, Serpini1 (GFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade I, member 1 (Serpini1), 200 uL, >10^7 TU/mL |
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|
200 µl /
€880.00
|
|
| OriGene Technologies, Inc. | ||
| MR206454L1V | Lenti ORF particles, Serpini1 (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade I, member 1 (Serpini1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€780.00
|
|
| OriGene Technologies, Inc. | ||
