NF-kB p105 / p50 antibody

Principal name

NF-kB p105 / p50 antibody

Alternative names for NF-kB p105 / p50 antibody

NFKB1, KBF1, EBP-1, EBP1, NF-kappa-B p50, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

SwissProt ID

P19838 (Human), P25799 (Mouse), Q04861 (Chick), Q63369 (Rat), Q6F3J0 (Canfa)

Gene ID

4790 (NFKB1), 18033 (Nfkb1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Functional assay (FN), ELISA (detection) (E(detection)), ChIP assay (ChIP), EPair for Elisa (EPair), Electrophoretic Mobility Shift Assay (EMSA)

Background of NF-kB p105 / p50 antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of sigl transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the D of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combitions act as transcriptiol activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translatiol modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an ictive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventiol activation pathway, I-kappa-B is phosphorylated by I-kappa-B kises (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptiol activators. The NF-kappa-B p50-p50 homodimer is a transcriptiol repressor, but can act as a transcriptiol activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslatiol processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translatiolly. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK sigling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

General readings

Heinemann,S., et al., (2006) J. Biol. Chem. 281 (13), 8565-8572

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NF-kB p105 / p50 antibody

MOLT4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-NF-κB p50 antibody (clone 4D1). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 4D1 Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NF-kB p105 / p50 antibody

Hela cell was stained by FITC-conjugated anti-NF-κB p50 Antibody Cat.-No AM06029FC-N (Clone 4D1). The nucleus is stained by DAPI. Mouse IgG1 4D1 FITC Hu ICC/IF
0.1 mg / €410.00
  Acris Antibodies GmbH

NF-kB p105 / p50 antibody

MOLT4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-NF-κB p50 antibody (clone 4D1). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 4D1 Aff - Purified Hu ICC/IF, P, WB
25 µg / €210.00
  Acris Antibodies GmbH

NF-kB p105 / p50 antibody

Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and bladder cancer tissues (right) using NFKB1 mouse mAb with DAB staining. Mouse IgG2a 5D10 Ascites Hu E, F, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

NF-kB p105 / p50 antibody

Immunohistochemical analysis of paraffin-embedded human intima cancer tissues (left) and human bladder cancer tissues (right) using NFKB1 mouse mAb with DAB staining. Mouse IgG2a 5D10D11 Ascites Hu E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

NF-kB p105 / p50 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between IKBKB and NFKB1. HeLa cells were stained with anti-IKBKB rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 2E6 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NF-kB p105 / p50 antibody

Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml] Mouse IgG1 4A11 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NF-kB p105 / p50 antibody

Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml] Mouse IgG1 3F6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NF-kB p105 / p50 antibody

Human tonsil stained with Anti-NFkB p50 antibody Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

NF-kB p105 / p50 antibody

Human tonsil stained with Anti-NFkB p50 antibody Rabbit Purified Hu P
1 ml / €440.00
  Acris Antibodies GmbH

NF-kB p105 / p50 antibody

Human tonsil stained with Anti-NFkB p50 antibody Rabbit Purified Hu P
0.1 ml / €180.00
  Acris Antibodies GmbH

NF-kB p105 / p50 antibody

All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.  The membrane was blocked in 5% dry milk for 2 h.  After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.  Washes with buffer were performed 4 times for 5' each.  The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h.   Washes with TBS preceded color development. Rabbit Serum Hu E, EMSA, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NF-kB p105 / p50 antibody

Western blot analysis of NF-kappaB p105/p50 expression in TNF-a treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NF-kB p105 / p50 antibody

Western blot analysis of lysates from Daudi, Jurkat cell line (from left to right), using NFKB1 Antibody(Cat. #TA328151). TA328151 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Mouse IgG1 1298CT792.105.117.133 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NF-kB p105 / p50 antibody

Western Blot: NFkB p105/p50 Antibody (2J10D7) [TA336417] - analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml. Mouse IgG1 2J10D7 Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NF-kB p105 / p50 antibody

Western Blot: NFkB p105 Antibody [TA336824] - WB analysis of NFkB in HepG2 whole cell lysate. Rabbit Purified Hu, Ms C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NF-kB p105 / p50 pSer337 antibody

NF-kB p105 / p50 Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NF-kB p105 / p50 pSer893 antibody

NF-kB p105 / p50 Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NF-kB p105 / p50 pSer907 antibody

Immunohistochemistry (IHC) analysis of NFκB-p105/p50 pSer907 antibody (Cat.-No.: AP01646PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NF-kB p105 / p50 pSer932 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NFκB-p105/p50 antibody (AP02503PU-S; AP02503PU-N). Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

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