NFAT2 / NFATC antibody

Principal name

NFAT2 / NFATC antibody

Alternative names for NFAT2 / NFATC antibody

NF-ATc1, NFATC1, NFATC, Nuclear factor of activated T-cells, cytoplasmic 1

SwissProt ID

O88942 (Mouse), O95644 (Human)

Gene ID

4772 (NFATC1)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Can (Canine), Chimp (Chimpanzee), GP (Guinea Pig), Bov (Bovine), Eq (Equine), Por (Porcine), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Paraffin Sections (P), Electrophoretic Mobility Shift Assay (EMSA), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ChIP assay (ChIP), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of NFAT2 / NFATC antibody

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively coordinate the immune response. Two forms of NFAT, the cytosolic (NFATc1) and the pre-existing NFATc2 (formerly NFATp), have been recognized on separate genes, and show sequence similarity with the Dorsal/Rel family of transcription factors. Studies indicate that NFATc is required for interleukin (IL-2) gene expression, is found primarily in lymphoid tissues and is induced upon T-cell activation. NFATc2 shows 73% homology with NFATc1 within the Rel-similarity region, but has a wider tissue distribution and does not appear to be inducible. T-cell activation requires two signals for initiation, one from the antigen receptor and the other by accessory cells or agents which activate protein kinase C. Upon binding of the antigen to the T-cell receptor, several membrane events are initiated which are similar to those initiated by many hormones, including the mobilization of intracellular calcium stores. NFATc1 appears to be independently modified by agents which activate protein kinase C or increase intracellular calcium, indicating one function of NFATc1 might be to integrate signals derived from different cell membrane receptors. Studies indicate that NFATc1 is also regulated by calcineurin, a calcium and calmodulin dependent protein phosphatase. Dephosphorylation is essential for NFATc activation, nuclear translocation, and early gene expression in T-cells. The immunosupressive drugs, cyclosporin A and FK506 when bound to the immunophilins cyclophilin and FKBP, respectively, inhibit dephosphorylation and activation of NFATc1 by calcineurin.

General readings

This product has been used in:Lyakh L et al. Expression of NFAT-family proteins in normal human T cells. Mol Cell Biol 17:2475-84 (1997). PubMed PMID: 9111316
Latinis KM et al. Two NFAT transcription factor binding sites participate in the regulation of CD95 (Fas) ligand expression in activated human T cells. J Biol Chem 272:31427-34 (1997). PubMed PMID: 9395475
Ho SN et al. NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity. J Biol Chem 270:19898-907 (1995). PubMed PMID: 7650004
Northrop JP et al. NF-AT components define a family of transcription factors targeted in T-cell activation. Nature 369:497-502 (1994). PubMed PMID: 8202141
General / background references:Chow CW et al. Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway. Science 278:1638-41 (1997). PubMed PMID: 9374467
Clipstone NA et al. Molecular analysis of the interaction of calcineurin with drug-immunophilin complexes. J Biol Chem 269:26431-7 (1994). PubMed PMID: 7523407
Crabtree GR Contingent genetic regulatory events in T lymphocyte activation. Science 243:355-61 (1989). PubMed PMID: 2783497
Shaw JP et al. Identification of a putative regulator of early T cell activation genes. Science 241:202-5 (1988). PubMed PMID: 3260404

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFAT2 / NFATC antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and esophagus cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human cervical and esophagus cancer tissue using NFATC1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFAT2 / NFATC antibody

Western blot analysis of extracts of various cell lines, using NFATC1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFAT2 / NFATC (197-304) antibody

Jurkat (Lane 1), human Th1 (Lane 2), and mouse Th1 (Lane 3) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-NFATc1 antibody (clone 7A6). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 7A6 Purified Hu, Ms, Rt F, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFAT2 / NFATC antibody

WB Suggested Anti-NFATC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Stomach Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFAT2 / NFATC antibody

Western Blot: NFAT2 Antibody [TA336469] - analysis of human Ramos (A) and mouse RAW (B) lysate using NFATc1 antibody at 1 ug/ml. Rabbit Purified Hu, Ms ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFAT2 / NFATC antibody

WB Suggested Anti-NFATC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta Rabbit IgG Purified GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFAT2 / NFATC antibody

WB Suggested Anti-NFATC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: NCI-H226 cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFAT2 / NFATC antibody

WB Suggested Anti-NFATC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFAT2 / NFATC antibody

WB Suggested Anti-NFATC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate; NFATC1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Can, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFAT2 / NFATC antibody

Immunohistochemistry: AM09061PU-N NFATC1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a AT1C3 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

NFAT2 / NFATC antibody

Immunohistochemistry: AM09061PU-N NFATC1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2a AT1C3 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

NFAT2 / NFATC (263-282) antibody

Thymus: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Purified Chk, Hu, L, Mky, Ms, Op, Prim, Rb, Rt, Tr, Xen P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NFAT2 / NFATC (197-304) antibody

Jurkat (Lane 1), human Th1 (Lane 2), and mouse Th1 (Lane 3) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-NFATc1 antibody (clone 7A6). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 7A6 Purified Hu, Ms, Rt F, ICC/IF, WB
25 µg / €240.00
  Acris Antibodies GmbH

NFAT2 / NFATC antibody

NFAT2 / NFATC Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NFAT2 / NFATC antibody

NFAT2 / NFATC Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

NFAT2 / NFATC antibody

Cell lysates of Ramos ( 20 µg ) were resolved by SDS-PAGE, transferred to NC membrane and probed with NFATC1 monoclonal antibody, clone AT1C3 ( Cat # MAB3209 ; 1 : 1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT1C3 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

NFAT2 / NFATC antibody

Human Placenta; WB Suggested Anti-NFATC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; NFATC1 antibody - N-terminal region (ARP38742_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Hu, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NFAT2 / NFATC antibody

Human NCI-H226; WB Suggested Anti-NFATC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: NCI-H226 cell lysate; NFATC1 antibody - middle region (ARP38743_P050) in Human NCI-H226 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

NFAT2 / NFATC (C-term) antibody

NFAT2 / NFATC Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

NFAT2 / NFATC antibody

Human Jurkat; WB Suggested Anti-NFATC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. NFATC1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; NFATC1 antibody - N-terminal region (ARP39979_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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