NFAT5 antibody

Principal name

NFAT5 antibody

Alternative names for NFAT5 antibody

NFAT5, KIAA0827, TONEBP, Nuclear factor of activated T-cells 5

SwissProt ID

D3ZGB1, O94916 (Human), Q9WV30

Gene ID

10725 (NFAT5), 54446

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Electrophoretic Mobility Shift Assay (EMSA), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of NFAT5 antibody

Members of the NFAT (nuclear factor of activated T cells) family of transcription factors are related to NFkB/Rel proteins and form cooperative complexes with the AP-1 proteins, Fos and Jun, on DNA to regulate cytokine expression in T cells. NFAT proteins are widely expressed and alternatively modified to generate splice variants, and they are localized to both the cytosol (NFATc) and to the nucleus (NFATn). NFAT1, NFAT2, and NFAT4 are predominantly expressed in immune cells, and NFAT2 and NFAT3 are expressed at high levels in cardiac tissues. In addition to activating cytokine gene transcription, NFAT2 is also implicated in cardiac valve development, and NFAT3 is involved in cardiac hypertrophy. NFAT5 is detected in both immune and nonimmune cells and, like other NFAT proteins, contains a highly conserved Rel-like binding domain that mediates NFAT proteins associating with specific consensus sequences on DNA. NFAT proteins are activated by increases in intracellular calcium, which leads to the calmodulin-dependent phosphatase, calcineurin, dephosphorylating NFAT proteins. This activating event induces a conformational change in the protein structure that exposes the nuclear localization signal and facilitates the translocation of NFAT proteins from the cytosol into the nucleus.

General readings

General / background references:Shen K & Meyer T Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation. Science 284:162-6 (1999). PubMed PMID: 10102820

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFAT5 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver and ovarian cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using NFAT5 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFAT5 antibody

Lanes:Lane 1: 20ug HT 29 lysate blocked with 5% FBS Lane 2: 20ug HT 29 lysate blocked with no FBS Lane 3: 20ug HT-29 lyaste blocked with no PBS + 100 mM NaCl; Primary Antibody Dilution:1:1000; Secondary Antibody:Goat anti-rabbit-HRP; Secondary Antibody Di Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFAT5 antibody

WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFAT5 antibody

NFAT5 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NFAT5 antibody

Western Blot analysis of NFAT5 expression in transfected 293T cell line (H00010725-T01) by NFAT5 MaxPab polyclonal antibody.

Lane 1: NFAT5 transfected lysate(163.24 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

NFAT5 antibody

NFAT5 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

NFAT5 pSer1197 antibody

Immunofluorescence analysis of COS7 cells, using NFAT5 (Phospho-Ser1197) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF
50 µg / €205.00
  Assay Biotech

NFAT5 pSer1197 antibody

Immunofluorescence analysis of COS7 cells, using NFAT5 (Phospho-Ser1197) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

NFAT5 pSer155 antibody

Western blot analysis of extracts from HeLa cells treated with forskolin 40nM 30', using NFAT5/TonEBP (Phospho-Ser155) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

NFAT5 pSer155 antibody

Western blot analysis of extracts from HeLa cells treated with forskolin 40nM 30', using NFAT5/TonEBP (Phospho-Ser155) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

NFAT5 antibody

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using NFAT5 (Ab-1197) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

NFAT5 antibody

Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using NFAT5 (Ab-1197) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

NFAT5 antibody

HT-29; . Lanes: Lane 1: 20ug HT 29 lysate blocked with 5% FBS Lane 2: 20ug HT 29 lysate blocked with no FBS Lane 3: 20ug HT-29 lyaste blocked with no PBS + 100 mM NaCl . Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-rabbit-HRP. Secondary Antibody Dilution: 1:1000. Gene Name: NFAT5. Submitted by: Manuel Fresno, CBMSO.; NFAT5 antibody - middle region (ARP37465_P050) in HT-29 cells using Western Blot Rabbit IgG Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NFAT5 antibody

Human MCF-7; WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; NFAT5 antibody - middle region (ARP38831_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

NFAT5 antibody

Western Blot: NFAT 5 Antibody [NB120-3446] - Figure 1 shows a Western blot of human NFAT5 from transfected BHK cell lysate with NB120-3446. Rabbit IgG Aff - Purified Hu EMSA, ICC/IF, IP, WB
50 µg / €400.00
  Novus Biologicals Inc.

NFAT5 antibody

NFAT5 Rabbit IgG Aff - Purified Hu, Ms E, P
0.1 mg / €360.00
  Novus Biologicals Inc.

NFAT5 antibody

NFAT5 Rabbit IgG Aff - Purified Hu, Ms E, P
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NFAT5

NFAT5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NFAT5

NFAT5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NFAT5 293T Cell Transient Overexpression Lysate(Denatured)

NFAT5 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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