NFE2L2 antibody

Principal name

NFE2L2 antibody

Alternative names for NFE2L2 antibody

NRF2,HEBP1, Nuclear factor erythroid 2-related factor 2

SwissProt ID

O54968 (Rat), Q16236 (Human), Q5NUA6 (Bovin), Q60795 (Mouse)

Gene ID

4780 (NFE2L2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ChIP assay (ChIP)

Background of NFE2L2 antibody

NFE2L2(nuclear factor (erythroid-derived 2)-like 2) also known as NRF2 or NFE2-RELATED TRANSCRIPTION FACTOR 2, is a transcription factor that in humans is encoded by theNFE2L2 gene. NFE2, NFE2L1, and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. NFE2L2 induces the expression of various genes including those that encode for several antioxidant enzymes, and it may play a physiological role in the regulation of oxidative stress. The NFE2L2 gene is located on 2q31.2. The identification of somatic mutations that disrupt the NRF2-KEAP1 interaction to stabilize NRF2 and increase the constitutive transcription of NRF2 target genes indicated that enhanced reactive oxygen species (ROS) detoxification and additional NRF2 functions may in fact be tumorigenic. Oncogene-directed increased expression of Nrf2 is a mechanism for the activation of the Nrf2 antioxidant program evident in primary cells and tissues of mice expressing KRas(G12D) and BRaf(V619E), and in human pancreatic cancer.

General readings

Padmanabhan,B., et al., (2006) Mol. Cell 21 (5), 689-700

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFE2L2 (C-term) antibody

AP15687PU NRF2 antibody staining of Human Placenta. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

NFE2L2 (C-term) antibody

AP15687PU NRF2 antibody staining of Human Placenta. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

NFE2L2 (C-term) antibody

AP15687PU NRF2 antibody staining of Human Placenta. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

NFE2L2 (445-458) antibody

(0.3ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFE2L2 antibody

Western blot analysis of extracts from HuvEc cells, using NRF2 antibody (#TA312825).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFE2L2 antibody

Predicted band size: 68 kDa . Positive control: Human fetal muscle tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFE2L2 antibody

Predicted band size: 68 kDa . Positive control: Mouse lung tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFE2L2 antibody

Western blot analysis of Nrf2 expression in HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFE2L2 antibody

Western blot analysis of kidney cell lysate using NFE2L2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFE2L2 antibody

Host: Rabbit; Target Name: NFE2L2; Sample Tissue: HT1080 Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFE2L2 antibody

Host: Rabbit; Target Name: Nfe2l2; Sample Tissue: Rat Thymus lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Hu, Ms, Rb, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFE2L2 antibody

WB Suggested Anti-NFE2L2 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: A172 cell lysateNFE2L2 is supported by BioGPS gene expression data to be expressed in A172 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFE2L2 antibody

Western blot analysis on HuvEc cell lysate using Nrf2 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFE2L2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFE2L2 (RC204140, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFE2L2. Mouse IgG2a OTI8A10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NFE2L2 (N-term) antibody

Western blot analysis of anti-NFE2L2 (N-term) (AP13999PU-N) in Ramos cell line lysates (35 µg/lane). Nrf2 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NFE2L2 antibody

Western blot analysis of NFE2L2 (arrow) using NFE2L2 Antibody (AP14000PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the NFE2L2 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NFE2L2 (569-588) antibody

Human Kidney (formalin-fixed, paraffin-embedded) stained with NFE2L2 antibody AP22716PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu P
0.25 ml / €470.00
  Acris Antibodies GmbH

NFE2L2 antibody

Detection limit for recombinant GST tagged NFE2L2 is approximately 3ng/ml as a capture antibody. Mouse IgG2a 2G7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NFE2L2 antibody

Detection limit for recombinant GST tagged NFE2L2 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2b 1B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NFE2L2 antibody

Western Blot analysis of NFE2L2 expression in transfected 293T cell line by NFE2L2 monoclonal antibody (M04), clone 3G7.

Lane 1: NFE2L2 transfected lysate(67.8 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 3G7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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