NFIA antibody

Principal name

NFIA antibody

Alternative names for NFIA antibody

NFI-A, NF-I/A, CCAAT-box-binding transcription factor, TGGCA-binding protein, CTG, Nuclear factor 1 A-type

SwissProt ID

P09414 (Rat), P17923 (Chick), Q02780 (Mouse), Q12857 (Human)

Gene ID

4774 (NFIA), 18027 (Nfia)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NFIA antibody

Nuclear factor I is a ubiquitous 47-kD dimeric DNA-binding protein whose recognition sequence, TGG(C/A)N(5)GCCAA, is found in the genomes of a number of DNA viruses. The same sequence occurs frequently in the human genome. The NFI protein stimulates initiation of adenovirus DNA replication in vitro and is capable of stimulating the transcription of genes in cooperation with other factors, such as the estrogen receptor. Santoro et al. (1988) gave a detailed analysis of NFI cDNAs isolated from the human, which, with similar analyses in other species, indicated that it is a member of a family of related proteins. Four known variants were referred to as CTF, X, L, and Red. These are the products of different genes, some of which (e.g., the first, CTF, later symbolized NFIC) are subject to differential splicing. Seisenberger et al. (1993) isolated 2 phages containing the second exon and flanking intron regions of the human gene for NFI/X, which has been designated NFIX. Qian et al. (1995) isolated partial cDNA sequences from 4 nuclear factor I proteins: NFIA, NFIB, NFIC, and NFIX.

General readings

Fazi,F., et al., (2005) Cell 123 (5), 819-831

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFIA antibody

Western blot analysis of extracts from rat brain cells and HUVEC cells, using Nuclear Factor 1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFIA antibody

NFIA Antibody (C-term) (Cat. #TA324538) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NFIA antibody detected the NFIA protein (arrow). Rabbit IgG Purified Chk, Hu, Ms, Rt ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NFIA antibody

Host: Rabbit; Target Name: Nfia; Sample Tissue: Mouse Brain lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFIA antibody

WB Suggested Anti-NFIA Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFIA antibody

WB Suggested Anti-Nfia Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFIA antibody

Immunohistochemistry (IHC) analysis of NF-1 antibody (Cat.-No.: AP06720PU-N) on paraffin-embedded sections. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NFIA antibody

NFIA MaxPab rabbit polyclonal antibody. Western Blot analysis of NFIA expression in mouse intestine. Rabbit Purified Hu, Ms, Rt E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NFIA antibody

Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 1E11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NFIA antibody

NFIA Mouse IgG1 1E11 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

NFIA antibody

Western blot analysis of extracts from rat brain/HuvEc cells, using Nuclear Factor 1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

NFIA antibody

Western blot analysis of extracts from rat brain/HuvEc cells, using Nuclear Factor 1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

NFIA antibody

Human Jurkat; WB Suggested Anti-NFIA Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; NFIA antibody - middle region (ARP32714_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

NFIA antibody

Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NFIA antibody

Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NFIA antibody

Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

NFIA antibody

NFIA Rabbit IgG Aff - Purified Ms WB
50 µg / €680.00
  Funakoshi Co., Ltd.

NFIA antibody

NFIA Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

NFIA antibody

NFIA Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

NFIA antibody

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81406] - Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells, glial cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

NFIA antibody

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81407] - Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

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