NFIA antibody

Principal name

NFIA antibody

Alternative names for NFIA antibody

NFI-A, NF-I/A, CCAAT-box-binding transcription factor, TGGCA-binding protein, CTG, Nuclear factor 1 A-type

SwissProt ID

P09414 (Rat), P17923 (Chick), Q02780 (Mouse), Q12857 (Human)

Gene ID

4774 (NFIA), 18027 (Nfia), 25492

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NFIA antibody

NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1,myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.

General readings

Fazi,F., et al., (2005) Cell 123 (5), 819-831

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFIA antibody

Western blot analysis of extracts from rat brain cells and HUVEC cells, using Nuclear Factor 1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFIA antibody

NFIA Antibody (C-term) (Cat. #TA324538) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NFIA antibody detected the NFIA protein (arrow). Rabbit IgG Purified Chk, Hu, Ms, Rt ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NFIA antibody

Host: Rabbit; Target Name: Nfia; Sample Tissue: Mouse Brain lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFIA antibody

WB Suggested Anti-NFIA Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFIA antibody

WB Suggested Anti-Nfia Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFIA antibody

Immunohistochemistry (IHC) analysis of NF-1 antibody (Cat.-No.: AP06720PU-N) on paraffin-embedded sections. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NFIA antibody

NFIA MaxPab rabbit polyclonal antibody. Western Blot analysis of NFIA expression in mouse intestine. Rabbit Purified Hu, Ms, Rt E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NFIA antibody

Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 1E11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NFIA antibody

NFIA Mouse IgG1 1E11 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

NFIA antibody

Western blot analysis of extracts from rat brain/HuvEc cells, using Nuclear Factor 1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

NFIA antibody

Western blot analysis of extracts from rat brain/HuvEc cells, using Nuclear Factor 1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

NFIA antibody

Human Jurkat; WB Suggested Anti-NFIA Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; NFIA antibody - middle region (ARP32714_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

NFIA antibody

Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NFIA antibody

Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NFIA antibody

Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

NFIA antibody

NFIA Rabbit IgG Aff - Purified Ms WB
50 µg / €680.00
  Funakoshi Co., Ltd.

CRISPR

Catalog No. Species Donor Vector  

Nfia - mouse gene knockout kit via CRISPR

Nfia - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nfia - mouse gene knockout kit via CRISPR

Nfia - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nfia - mouse gene knockout kit via CRISPR

Nfia - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NFIA (transcript variant 2)

NFIA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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