NFKBIA / IKBA antibody

Principal name

NFKBIA / IKBA antibody

Alternative names for NFKBIA / IKBA antibody

I-kappa-B-alpha, MAD3, NFKBI, I kappa B-alpha, IkappaBalpha, IkB-alpha, NF-kappa-B inhibitor alpha, Major histocompatibility complex enhancer-binding protein MAD3

SwissProt ID

P25963 (Human), Q08353 (Pig), Q63746 (Rat), Q91974 (Chick), Q9Z1E3 (Mouse)

Gene ID

4792 (NFKBIA), 18035 (Nfkbia)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine), Can (Canine), Chk (Chicken), Sh (Sheep), Mky (Monkey)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Electrophoretic Mobility Shift Assay (EMSA), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NFKBIA / IKBA antibody

Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.

General readings

J Cell Biol. 1996 Jun;133(5):1083-93.
Inhibition of I kappa B-alpha phosphorylation and degradation and subsequent NF-kappa B activation by glutathione peroxidase overexpression.
Kretz-Remy C, Mehlen P, Mirault ME, Arrigo AP.
Centre National de la Recherche Scientifique Unit

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFKBIA / IKBA antibody

IKB alpha antibody detects IKB alpha protein by Western blot analysis. A. 30 ug BCL-1 whole cell lysate/extract. B. 30 ug C2C12 whole cell lysate/extrac. 10 % SDS-PAGE. IKB alpha antibody (TA308928) dilution: 1:5000 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Western blot analysis of HL60 cell lysate using NFKBIA antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NFKBIA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA507178) Mouse IgG1 OTI2F5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NFKBIA / IKBA antibody

Western blot analysis of extracts from HuvEc cells, treated with TNF-a 20ng/ml 30', using IkappaB-alpha (Ab-42) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Western blot analysis of extracts from MCF7 cells, treated with TNF-a, using IkappaB-alpha (Ab-32/36) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Western blot analysis of extracts from HuvEc cells, using I?B-a antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Predicted band size: 36kDa. Positive control: Hela, Jurkat, 293T, NIH/3T3, K562 and A172 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1-6:Hela, Jurkat, 293T, NIH/3T3, K562 and A172 cell lysate Lysates: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

NFKBIA Antibody (Cat. #TA325169) western blot analysis in Hela,mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the NFKBIA antibody detected the NFKBIA protein (arrow). Mouse IgG1 1121CT8.6.1 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

NFKBIA / IKBA Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Western blot analysis of IkappaB-alpha expression in HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Western blot analysis of extracts from MCF7 cells, treated with TNF-?, using IkappaB-alpha Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Western Blot: IKB alpha Antibody (6A920) [TA336372] - IKB alpha Antibody (6A920) [TA336372] - Western blot analysis of IkBa using TA336372 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate. Mouse IgG1 6A920 Purified Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Western Blot: IKB alpha Antibody (5D1623) [TA336424] - Detection of cleaved IkBalpha in Jurkat cell lysates. Lane A. TA336424 incubated in the presence of the immunizing peptide. Lane B. incubated with the antibody alone. Mouse IgG1 5D1623 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Western Blot: IKB alpha [p Ser32, p Ser36] Antibody (39A1413) [TA336464] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) fo Mouse IgG1 39A1413 Purified Bov, Can, Hu, Ms, Por, Rt IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Western blot detection of IkB-alpha(N-terminus) in Jurkat and Hela cell lysates using IkB-alpha(N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 36KDa.Observed band size: 36KDa. Mouse 2E10-B11-F6 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Immunofluorescent staining of HeLa cells using anti-NFKBIA mouse monoclonal antibody (TA507172). Mouse IgG1 OTI1D4 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NFKBIA / IKBA antibody

ELISA: IKB alpha Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

NFKBIA / IKBA antibody

Western blot analysis of IκBα in Jurkat cells treated with (right lane) or without (left lane) TNF-α (1 nM). Mouse IgG1 6A055 Purified Hu IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

NFKBIA / IKBA antibody

NFKBIA / IKBA Rabbit Aff - Purified Ms WB
0.1 mg / €370.00
  Acris Antibodies GmbH

NFKBIA / IKBA pSer32/36 antibody

Immunohistochemistry (IHC) analysis of IkB-alpha pSer32/36 antibody (Cat.-No.: AP01617PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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