NFKBIE / IKBE antibody

Principal name

NFKBIE / IKBE antibody

Alternative names for NFKBIE / IKBE antibody

I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon, NF-kappa-B inhibitor epsilon

SwissProt ID

O00221 (Human), O54910 (Mouse)

Gene ID

4794 (NFKBIE)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of NFKBIE / IKBE antibody

The transcription factor NFkappaB is retained in the cytoplasm in an inactive form by the inhibitory protein IkappaB. Activation of NFkappaB requires that IkappaB be phosphorylated on specific serine residues, which results in targeted degradation of IkappaB. IkappaB kinase alpha (IKKalpha), previously designated CHUK, interacts with IkappaB-alpha and specifically phosphorylates IkappaB-alpha on the sites that trigger its degradation Serines 32 and 36. IKKalpha appears to be critical for NFkappaB activation in response to proinflammatory cytokines. Phosphorylation of IkappaB by IKKalpha is stimulated by the NFkappaB inducing kinase (NIK), which itself is a central regulator for NFkappaB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKgamma (also designated NEMO), and each appear to make essential contributions to IkappaB phosphorylation.

General readings

Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74
Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663.
Chen, Z.J. et al. (1996) Cell; 84, 853-862.
Brown, K. et al. (1995) Science; 267, 1485-1488.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFKBIE / IKBE antibody

Western blot analysis of extracts from Hela cells treated with TNF-a (20ng/ml 5min), using I?B-e (Ab-22) antibody (#TA314256, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIE / IKBE antibody

Predicted band size: 45 kDa . Positive control: Raji cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIE / IKBE antibody

Predicted band size: 45 kDa . Positive control: PC3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIE / IKBE antibody

Western blot analysis of IkappaB-epsilon expression in TNF-a treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFKBIE / IKBE antibody

WB Suggested Anti-NFKBIE Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFKBIE / IKBE pSer22 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-ε antibody (AP02473PU-N). Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

NFKBIE / IKBE pSer22 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-ε antibody (AP02473PU-N). Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

NFKBIE / IKBE antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-ε antibody. Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

NFKBIE / IKBE antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-ε antibody. Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €230.00
  Acris Antibodies GmbH

NFKBIE / IKBE antibody

Immunohistochemistry (IHC) analyzes of IκB-ε antibody (Cat.-No.: Ap06186PU-N) om paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NFKBIE / IKBE antibody

NFKBIE / IKBE Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NFKBIE / IKBE antibody

NFKBIE / IKBE Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

NFKBIE / IKBE antibody

NFKBIE / IKBE Mouse IgG2a 3F12 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NFKBIE / IKBE pSer22 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

NFKBIE / IKBE pSer22 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

NFKBIE / IKBE antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IkappaB-epsilon (Ab-22) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

NFKBIE / IKBE antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IkappaB-epsilon (Ab-22) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

NFKBIE / IKBE antibody

Human HT1080; WB Suggested Anti-NFKBIE Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate; NFKBIE antibody - middle region (ARP38497_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NFKBIE / IKBE antibody

Western blot analysis of IKB epsilon expression in Jurkat (A); HeLa (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NFKBIE / IKBE antibody

Western blot analysis of IKB epsilon expression in Jurkat (A); HeLa (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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