NFX1 antibody

Principal name

NFX1 antibody

Alternative names for NFX1 antibody

NFX2, Transcriptional repressor NF-X1

SwissProt ID

Q12986 (Human)

Gene ID

4799 (NFX1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of NFX1 antibody

MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.

General readings

Katzenellenbogen,R.A., (2007) J. Virol. 81 (8), 3786-3796

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFX1 antibody

WB Suggested Anti-NFX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFX1 antibody

WB Suggested Anti-NFX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate NFX1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFX1 antibody

NFX1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NFX1 antibody

Immunofluorescence of monoclonal antibody to NFX1 on HeLa cell. [antibody concentration 30 ug/ml] Mouse IgG2a 1D12 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NFX1 antibody

Human Jurkat; WB Suggested Anti-NFX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. NFX1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; NFX1 antibody - N-terminal region (ARP34770_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NFX1 antibody

Human Testis; Immunohistochemistry with Human Testis lysate tissue; NFX1 antibody - middle region (P100680_P050) in Human Testis cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

NFX1 antibody

Western Blot: NFX1 Antibody [NBP1-49931] - Detection of Human NFX1 by Western Blot and Immunoprecipitation.  Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-NFX1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  NFX1 was also immunoprecipitated by rabbit anti-NFX1 antibodies, which recognize downstream epitopes.  For blotting immunoprecipitated NFX1 was used.   Detection: Chemiluminescence with exposure times of 3 minutes (A and B). Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

NFX1 antibody

Western Blot: NFX1 Antibody [NBP1-49932] - Detection of Human NFX1 by Western Blot of Immunoprecipitates.  Samples:  Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-NFX1 antibody used for IP at 3 mcg/mg lysate.  NFX1 was also immunoprecipitated by rabbit anti-NFX1 antibodies which recognize other epitopes.  For blotting immunoprecipitated NFX1 was used at 0.4 mcg/ml.   Detection: Chemiluminescence with an exposure time of 10 seconds. Rabbit Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

NFX1 antibody

Western Blot: NFX1 Antibody [NBP1-49933] - Detection of Human and Mouse NFX1 by Western Blot (h&m) and Immunoprecipitation (h).  Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-NFX1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  NFX1 was also immunoprecipitated by rabbit anti-NFX1 antibodies which recognize upstream epitopes.  For blotting immunoprecipitated NFX1 was used.   Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B). Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NFX1

NFX1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NFX1

NFX1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NFX1 overexpression lysate

NFX1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

12 Item(s)

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