NFYA antibody

Principal name

NFYA antibody

Alternative names for NFYA antibody

Nuclear transcription factor Y subunit alpha

SwissProt ID

P23511 (Human)

Gene ID

4800 (NFYA)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NFYA antibody

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq]. Transcript Variant: This variant (2) is missing alternate exon B, resulting in the short isoform, isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Zhu,Q.S., et al., (2004) J.Biol.Chem.279(29),29902-29910

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFYA antibody

Western blot analysis of extracts from 293 cells, HepG2 cells and COLO205 cells, using NFYA antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFYA antibody

WB of Rabbit Anti-NF-Y(A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Rabbit Purified Hu, Ms E, EMSA, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFYA antibody

WB of Rabbit Anti-NF-Y (A Subunit) Antibody. Lane 1: CHO-7 cells were cultured in the absence (I) of cholesterol. Lane 2: CHO-7 cells were cultured in the presence (S) of cholesterol. Load: 35 µg per lane. Primary antibody: NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Rabbit Serum Hu, Ms E, EMSA, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFYA (N-term) antibody

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Supernatant Hu E, ICC/IF, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

NFYA antibody

NFYA Rabbit Purified Hu, Ms E, EMSA, IP, WB
0.1 mg / €530.00
  Acris Antibodies GmbH

NFYA antibody

NFYA Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NFYA antibody

CHO-7 cells were cultured in the absence ( I ) or presence ( S ) of cholesterol.
Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein were subjected to analysis by SDS-PAGE and immunoblot.
Using NFYA polyclonal antibody ( Cat # PAB11297 ) at a 1 : 1000 dilution. Rabbit Hu E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

NFYA antibody

Western blot analysis of extracts from HepG2/293/COLO205 cells, using NFYA Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

NFYA antibody

Western blot analysis of extracts from HepG2/293/COLO205 cells, using NFYA Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

NFYA antibody

Human Liver; NFYA antibody - C-terminal region (ARP31263_T100) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

NFYA antibody

Human Liver; NFYA antibody - C-terminal region (ARP31380_P050) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

NFYA antibody

Immunohistochemical analysis of NFYA staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NFYA antibody

Immunohistochemical analysis of NFYA staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NFYA antibody

Immunohistochemical analysis of NFYA staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

NFYA antibody

NFYA Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

NFYA antibody

NFYA Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NFYA (full length, N-term HIS tag, transcript variant 1)

NFYA Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

NFYA

NFYA Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NFYA

NFYA Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

NFYA

NFYA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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