NFYA antibody

Principal name

NFYA antibody

Alternative names for NFYA antibody

Nuclear transcription factor Y subunit alpha

SwissProt ID

P23511 (Human)

Gene ID

4800 (NFYA)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NFYA antibody

NFY is a transcription factor composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. NFY binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit.
NFY recognizes a CCAAT motif upstream of gene promoters and is probably involved in the regulation of a variety of genes.
Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NFY in DNA binding assays. The conserved core sequences of NFYB and NFYC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.

General readings

Zhu,Q.S., et al., (2004) J.Biol.Chem.279(29),29902-29910

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFYA antibody

Western blot analysis of extracts from 293 cells, HepG2 cells and COLO205 cells, using NFYA antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFYA antibody

WB of Rabbit Anti-NF-Y(A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Rabbit Purified Hu, Ms E, EMSA, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFYA antibody

WB of Rabbit Anti-NF-Y (A Subunit) Antibody. Lane 1: CHO-7 cells were cultured in the absence (I) of cholesterol. Lane 2: CHO-7 cells were cultured in the presence (S) of cholesterol. Load: 35 µg per lane. Primary antibody: NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Rabbit Serum Hu, Ms E, EMSA, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFYA antibody

WB Suggested Anti-NFYA Antibody Titration: 5.0-8.0ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NFYA antibody

WB Suggested Anti-NFYA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFYA (N-term) antibody

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Supernatant Hu E, ICC/IF, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

NFYA antibody

NFYA Rabbit Purified Hu, Ms E, EMSA, IP, WB
0.1 mg / €530.00
  Acris Antibodies GmbH

NFYA antibody

NFYA Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NFYA antibody

CHO-7 cells were cultured in the absence ( I ) or presence ( S ) of cholesterol.
Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein were subjected to analysis by SDS-PAGE and immunoblot.
Using NFYA polyclonal antibody ( Cat # PAB11297 ) at a 1 : 1000 dilution. Rabbit Hu E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

NFYA antibody

Western blot analysis of extracts from HepG2/293/COLO205 cells, using NFYA Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

NFYA antibody

Western blot analysis of extracts from HepG2/293/COLO205 cells, using NFYA Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

NFYA antibody

Human Liver; NFYA antibody - C-terminal region (ARP31263_T100) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

NFYA antibody

Human Liver; NFYA antibody - C-terminal region (ARP31380_P050) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

NFYA antibody

Immunohistochemical analysis of NFYA staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NFYA antibody

Immunohistochemical analysis of NFYA staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NFYA antibody

Immunohistochemical analysis of NFYA staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NFYA (full length, N-term HIS tag, transcript variant 1)

NFYA Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

NFYA

NFYA Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NFYA

NFYA Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

NFYA

NFYA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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