NFYB antibody

Principal name

NFYB antibody

Alternative names for NFYB antibody

HAP3, Nuclear transcription factor Y subunit beta

SwissProt ID

P25208 (Human), P63139 (Mouse), P63140 (Rat)

Gene ID

4801 (NFYB)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Electrophoretic Mobility Shift Assay (EMSA)

Background of NFYB antibody

NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

General readings

Kimura,K., (2006) Genome Res. 16 (1), 55-65

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NFYB antibody

Immunohistochemistry. Anti-NF-Y (B Subunit) antibody was diluted 1:500 to detect NF-YB in human thymus tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Rabbit Serum Hu, Ms E, EMSA, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NFYB antibody

NFYB Antibody (N-term) (Cat. #TA324547) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow). Rabbit IgG Purified Bov, Chk, Hu, Ms, Rt ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NFYB antibody

WB Suggested Anti-NFYB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NFYB antibody

Figure 1. Staining NFYB in Thymus by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Hu E, ICC/IF, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

NFYB antibody

NFYB Rabbit Ig Fraction Hu, Ms E, EMSA, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

NFYB antibody

NFYB Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NFYB antibody

Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.

Lane 1: NFYB transfected lysate(22.8 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 6H6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NFYB antibody

Detection limit for recombinant GST tagged NFYB is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 6E4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NFYB antibody

Detection limit for recombinant GST tagged NFYB is approximately 0.03ng/ml as a capture antibody. Mouse IgG2a 5D1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NFYB antibody

Detection limit for recombinant GST tagged NFYB is approximately 0.3ng/ml as a capture antibody. Mouse IgG2b 1G10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NFYB antibody

NFYB Mouse IgG2a 2H2 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

NFYB antibody

Western blot analysis of extracts from 293 cells, using NFYB Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

NFYB antibody

Western blot analysis of extracts from 293 cells, using NFYB Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

NFYB antibody

Transfected 293T; WB Suggested Anti-NFYB Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; NFYB antibody - middle region (ARP31262_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

NFYB antibody

Transfected 293T; WB Suggested Anti-NFYB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; NFYB antibody - N-terminal region (ARP38748_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NFYB antibody

NFYB Rabbit Hu E, EMSA, ICC/IF, IP, P, WB
50 µl / €480.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NFYB

NFYB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

NFYB

NFYB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NFYB

NFYB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

19 Item(s)

per page
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