NGF antibody

Principal name

NGF antibody

Alternative names for NGF antibody

Beta-NGF, NGFB,Beta-nerve growth factor

SwissProt ID

P01138 (Human), P01139 (Mouse), P05200 (Chick), P13600 (Bovin), P25427 (Rat), Q29074 (Pig)

Gene ID

4803 (NGF), 18049 (Ngf), 310738

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse), Bov (Bovine), Viper, Av (Birds), Eq (Equine)

Available hosts

Goat, Rabbit, Mouse, Sheep

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Neutralisation (NEUT), Immunoprecipitation (IP), Dot blot (Dot)

Background of NGF antibody

Beta-NGF, also known as nerve growth factor beta-1, NGF-b1, or NGF-beta, is a neurotrophic factor that influences the growth and differentiation of sympathetic and sensory neurons. Beta-NGF, comprised alpha, beta, and gamma subunits, is a 26 kDa noncovalently associated homodimer with 2 potential N-linked glycosylation sites. Beta-NGF regulates the growth, development, survival and repair of the vertebrate nervous system. Specifically, it is involved in the survival, differentiation and functional activities of sensory and sympathetic neurons in the peripheral nervous system. Beta-NGF is also involved in the developmental and functional activities of cholinergic neurons in the central nervous system and induces the expression of a number of neurotransmitter peptides in sensory neurons such as substance P somatostatin, an inhibitor of growth hormone secretion, and vasoactive intestinal peptide, a potent vasodilator. Beta-NGF is also well documented to be involved in mediating pain perception. In addition to its role in the nervous system, beta-NGF has been implicated in immune responses. For example, beta-NGF is induced by the pro-inflammatory cytokines IL-1, IL-6, TNF-alpha and TGF-beta. Furthermore beta-NGF stimulates chemotaxis of neutrophils and proliferation of B cells, T cells and mast cells. It also is involved in B cell differentiation and inhibits immunoglobulin production from plasma cells. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

General / background references:Selby MJ et al. Mouse nerve growth factor gene: structure and expression. Mol Cell Biol 7:3057-64 (1987). PubMed PMID: 3670305
Zabel BU et al. Chromosomal locations of the human and mouse genes for precursors of epidermal growth factor and the beta subunit of nerve growth factor. Proc Natl Acad Sci U S A 82:469-73 (1985). PubMed PMID: 3871525
Francke U et al. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. Science 222:1248-51 (1983). PubMed PMID: 6648531

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NGF antibody

Formalin-Fixed, Paraffin-Embedded sections of Human pancreas adenocarcinoma stained with beta-NGF Antibody Cat.-No PP1057P at 0.5 µg/ml with an overnight incubation at 4˚C.  An HRP-labeled polymer detection system was used with a DAB chromogen. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

NGF antibody

Formalin-Fixed, Paraffin-Embedded sections of Human pancreas adenocarcinoma stained with beta-NGF Antibody Cat.-No PP1057P at 0.5 µg/ml with an overnight incubation at 4˚C.  An HRP-labeled polymer detection system was used with a DAB chromogen. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

NGF antibody

Predicted band size: 27 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NGF antibody

Western blot analysis of extracts of mouse liver tissue, using NGF antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NGF antibody

To detect Human beta-NGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NGF antibody

To detect Human beta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (TA328404) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta-NGF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NGF antibody

WB analysis using Anti-mNGF antibody (1:200). Immunohistochemistry Expression of NGF in rat brain. IHC staining of rat hippocampal mossy fiber terminal zone using Anti-mNGF antibody . A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

NGF antibody

Western blot analysis of proNGF (WT-mouse) , (Lanes 1-3) and proNGF (WT-human) , (Lanes 4-6): 1, 4. 100 ng + Anti-proNGF antibody (ANT-005), (1:200). 2, 5. 250 ng + Anti-proNGF antibody (1:200). 3, 6. 500 ng + Anti-proNGF antibody (1:200). Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

NGF antibody

Western blot analysis of NGF beta expression in Jurkat cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NGF antibody

NGF Mouse IgG2b Purified Hu E, FN, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

NGF antibody

NGF Mouse IgG1 AS18 Biotin Bov, Hu, Ms, Viper E
0.2 mg / €400.00
  Acris Antibodies GmbH

NGF antibody

NGF Mouse IgG1 AS18 Purified Bov, Hu, Ms, Viper E, FN, IP
0.5 mg / €400.00
  Acris Antibodies GmbH

NGF antibody

NGF Mouse IgG1 AS21 Purified Bov, Hu, Ms, Viper E, FN
0.5 mg / €400.00
  Acris Antibodies GmbH

NGF antibody

NGF Mouse IgG1 AS22 Purified Bov, Hu, Ms, Viper E, FN
0.5 mg / €400.00
  Acris Antibodies GmbH

NGF antibody

NGF Rabbit IgG Serum Hu, Ms E, FN
1 ml / €370.00
  Acris Antibodies GmbH

NGF antibody

NGF Rabbit IgG Purified Hu, Ms E, FN, WB
1 mg / €380.00
  Acris Antibodies GmbH

NGF antibody

NGF Rabbit IgG Serum Ms
0.1 ml / €520.00
  Acris Antibodies GmbH

NGF antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with NGFB antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

NGF antibody

Immunohistochemistry analysis of NGF Antibody (Cat.-No AP20675PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NGF antibody

Immunohistochemical analysis of paraffin embedded rat tissue sections (brain), staining NGFbeat in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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