Nibrin antibody

Principal name

Nibrin antibody

Alternative names for Nibrin antibody

NBN, NBS, NBS1, P95, Nijmegen breakage syndrome protein 1, Cell cycle regulatory protein p95

SwissProt ID

O60934 (Human), Q9DE07 (Chick), Q9JIL9 (Rat), Q9R207 (Mouse)

Gene ID

4683 (NBN), 27354 (Nbn), 85482

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C)

Background of Nibrin antibody

NBS1 (Nijmegen breakage syndrome protein 1) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays important role in detection and sigling of D double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint sigling, and as repair-effector in two competing DSB repair pathways: homologous recombition (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintence. NBS1's FHA domain binds phospho-Thr residues in Ser-X-Thr motifs present in D damage proteins, including Mdc1 and Ctp1, whereas, BRCT domains of NBS1 bind Ser-X-Thr motifs when Ser residue is phosphorylated. These phospho-dependent interactions are important for recruiting repair and checkpoint proteins to DSB sites and NBS1 is responsible for MRN complex's nuclear translocation. NBS1 itself does not possess enzymatic activity and contributes to DSB repair primarily by mediating protein-protein interactions at D breakage sites. The central region of NBS1 1 possesses several SQ motifs that are phosphorylated by ATM kise via D damage response. NBS1's C-terminus contains a domain that interacts with ATM and recruits it to DSBs, and induces apoptosis in response to damage. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombition etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis.

General readings

1. Lee JH  et al. Direct Activation of ATM by Resveratrol under Oxidizing Conditions. PLoS One9:e97969 (2014). WB ; Human .PubMed: 24933654
2. Horton JK  et al. Requirement for NBS1 in the S phase checkpoint response to DNA methylation combined with PARP inhibition. DNA Repair (Amst) 10:225-34 (2011). WB ; Human .PubMed: 21130714
3. Reiman A  et al. Lymphoid tumours and breast cancer in ataxia telangiectasia; substantial protective effect of residual ATM kinase activity against childhood tumours. Br J Cancer105:586-91 (2011). WB ; Human .PubMed: 21792198
4. Weston VJ  et al. The PARP inhibitor olaparib induces significant killing of ATM-deficient lymphoid tumor cells in vitro and in vivo. Blood 116:4578-87 (2010). WB ; Human .PubMed: 20739657

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nibrin (C-term) antibody

Immunofluorescence microscopy of Hela cells labeled with anti-NBS 1 (poly6138) (red).  a-tubulin (clone 10D8) was also labeled (green); nuclei were stained with DAPI (blue).View Protocol Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Nibrin (C-term) antibody

Immunofluorescence microscopy of Hela cells labeled with anti-NBS 1 (poly6138) (red).  a-tubulin (clone 10D8) was also labeled (green); nuclei were stained with DAPI (blue).View Protocol Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
50 µl / €190.00
  Acris Antibodies GmbH

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB99 Purified Hu, Mky P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (UM800030; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB100 Purified Hu, Mky P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB99 Purified Hu, Mky P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (UM800030; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB100 Purified Hu, Mky P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801215) Mouse IgG1 OTI1F11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801216) Mouse IgG1 OTI2E9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801217) Mouse IgG1 OTI1G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801218) Mouse IgG1 OTI3A2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801251) Mouse IgG2a OTI3A9 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801388) Mouse IgG1 OTI8E6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801389) Mouse IgG1 OTI6D10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801390) Mouse IgG1 OTI2G4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801410) Mouse IgG1 OTIE7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801421) Mouse IgG2b OTI2F5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801424) Mouse IgG1 OTI5E11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801427) Mouse IgG1 OTI2E10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801448) Mouse IgG2a OTI3C12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Nibrin antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801520) Mouse IgG1 OTI9H2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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