Nicastrin antibody

Principal name

Nicastrin antibody

Alternative names for Nicastrin antibody

NCSTN, KIAA0253, UNQ1874/PRO4317

SwissProt ID

P57716 (Mouse), Q8CGU6 (Rat), Q92542 (Human)

Gene ID

23385 (NCSTN)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Nicastrin antibody

Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide.
Also found in the neurofibrillary lesions associated with Alzheimer's disease is a protein termed Tau. Tau is a neuronal microtubule-associated protein found predominantly on axons. Tau functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major constituent of paired helical filaments (PHF), which are the building block of neurofibrillary lesions found in brain tissue of Alzheimer's diseased patients.

General readings

Neuron, 27(3):419-22, 2000.
Neuropathology, 21(2):115-22, 2001.
JBC Vol. 279 No. 30, 31329-31336, Jul 2004.
J. Biol. Chem., Vol 278: 26687 - 26694, Jul 2003.
PNAS 99(13):8666-8671, 2002.
J. Biol. Chem., Oct 2006; 281: 32240 - 32253.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nicastrin (C-term) antibody

Western blot analysis of Nicastrin in mouse brain tissue lysate with Nicastrin antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nicastrin antibody

Western blot analysis of Nicastrin in human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nicastrin antibody

Western blot analysis of brain cell and C6 cell lysate using NCSTN antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nicastrin antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Nicastrin antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cortex. Rabbit Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Nicastrin Control Peptide

Nicastrin
50 µg / €160.00
  Acris Antibodies GmbH

Nicastrin Control Peptide

Nicastrin
50 µg / €160.00
  Acris Antibodies GmbH

Nicastrin antibody

NCSTN polyclonal antibody (A01), Lot # 051214JC01. Western Blot analysis of NCSTN expression in NIH/3T3. Mouse Serum Hu, Ms E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Nicastrin antibody

Western Blot analysis of NCSTN expression in transfected 293T cell line (H00023385-T01) by NCSTN MaxPab polyclonal antibody.

Lane 1: NCSTN transfected lysate(75.79 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Nicastrin antibody

NCSTN MaxPab rabbit polyclonal antibody. Western Blot analysis of NCSTN expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Nicastrin antibody

Nicastrin Mouse IgG2a 1E8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Nicastrin antibody

Western blot analysis of mouse brain tissue extracts ( 80 µg ) and probed with NCSTN monoclonal antibody, clone AT3B2 ( Cat # MAB4200 ; 1 : 500 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT3B2 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Nicastrin antibody

Immunohistochemistry of NCSTN in human brain tissue with NCSTN polyclonal antibody ( Cat # PAB13231 ) at 5 µg/ml. Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Nicastrin antibody

Immunohistochemistry of NCSTN in human brain tissue with NCSTN polyclonal antibody ( Cat # PAB13232 ) at 2.5 µg/ml. Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Nicastrin antibody

Nicastrin Mouse IgG2b AT3B2 Hu, Ms E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Nicastrin antibody

Immunohistochemistry-Paraffin: Nicastrin Antibody [NBP1-77269] - Human brain tissue with Nicastrin antibody at 5 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Nicastrin antibody

Immunohistochemistry-Paraffin: Nicastrin Antibody [NBP1-77270] - Human brain tissue with Nicastrin antibody at 2.5 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Nicastrin

Nicastrin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Nicastrin

Nicastrin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Nicastrin

Nicastrin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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