NIP3 antibody

Principal name

NIP3 antibody

Alternative names for NIP3 antibody

BNIP3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

SwissProt ID

O55003 (Mouse), Q12983 (Human), Q32KN2 (Bovin)

Gene ID

664 (BNIP3), 662 (BNIP1)

Available reactivities

Hu (Human), Fe (Feline), Chk (Chicken), Ms (Mouse), Rt (Rat), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Dot blot (Dot), ELISA (detection) (E(detection))

Background of NIP3 antibody

BNIP3, formerly NIP3 (nineteen kDa interacting protein-3), is a pro-apoptotic, mitochondrial protein classified in the Bcl 2 family based on limited sequence homology to the Bcl 2 homology 3 (BH3) domain (amino acids 110-118) and C-terminal TM domain. BNIP3 expressed in yeast and mammalian cells interacts with survival promoting proteins Bcl 2, Bcl XL, CED9 and the adenovirus E1B 19K protein. Typically the BH3 domain of pro-apoptotic Bcl-2 homologues mediates Bcl 2/Bcl XL heterodimerization and confers pro-apoptotic activity. BNIP3 represents a subfamily of Bcl 2 related proteins, which functions without a typical BH3 domain to regulate apoptosis from both mitochondrial and nonmitochondrial sites by selective Bcl 2/Bcl XL interactions. The N-terminus (residues 1-49) and the C-terminus TM domain of BNIP3 are critical for Bcl 2 heterodimerization, and either region is sufficient for Bcl XL interaction. The TM domain of BNIP3 is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting. BNIP3 contains PEST sequences suggesting that the protein may be susceptible to rapid degradation by proteases. PEST sequences commonly contain high local concentrations of amino acids P, E, S, T, and D flanked by charged amino acids and these are abundantly present in NIP3. Thus, the posttranslational control of BNIP3 expression through rapid protein degradation may constitute a mechanism for regulating the intracellular levels of a potentially lethal protein. Homologues of BNIP3 sharing both structural and functional similarity have been identified in mammals: Nix (also called BNIP3L/BNIP3alpha\/B5) and, in C. elegans, ceBNIP3. The TM domain of BNIP3 and Nix share 80% identity. Endogenous BNIP3 is loosely associated with mitochondrial membrane in normal tissue but fully integrates into the mitochondrial outer membrane with the N-terminus in the cytoplasm and the C-terminus in the membrane during induction of cell death. This is accompanied by rapid and profound mitochondrial dysfunction characterized by opening of the mitochondrial permeability transition (PT) pore, proton electrochemical gradient suppression, and increased reactive oxygen species production. BNIP3 has been reported to localize to the nuclear envelope when co-expressed with E1B 19K. Endogenous BNIP3 protein is abundant in murine andhuman skeletal muscle and is not detectable in lysates of all other nonskeletal muscle-bearing tissues and many cell lines, including myoblasts and differentiated myocytes. BNIP3 also plays a role in autophagy. Hypoxia-induced autophagy requires expression of this protein. Beclin, an autophagy protein, is kept in check by Bcl2 (normoxic). When Bcl2 lets go of Beclin it then binds BNIP3/L3 (hypoxic), causing autophagy.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NIP3 (BH3 Domain) antibody

The anti-NIP3 BH3 domain Pab (Cat. #TA302077) is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2). Rabbit Ig Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NIP3 antibody

Western Blot: BNIP3 Antibody [TA336539] - detection of BNIP3 in hypoxic Hep3b lysate using TA336539. Rabbit Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NIP3 antibody

Immunohistochemistry: BNIP3 Antibody [TA336842] - Analysis of BNIP3 in mouse kidney using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NIP3 (BH3 Domain) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NIP3 antibody

Immunohistochemistry analyzes of BNIP-3 antibody (Cat.-No.: AP20658PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

NIP3 (C-term) antibody

Immunohistochemical analysis of paraffin embedded cancer sections, staining in nucleus, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

NIP3 antibody

Western Blot analysis of BNIP3 expression in transfected 293T cell line (H00000664-T05) by BNIP3 MaxPab polyclonal antibody.

Lane 1: BNIP3 transfected lysate(21.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NIP3 antibody

NIP3 Rabbit Chk, Hu, Ms, Rt C, IP, P
50 µl / €400.00
  Abnova Taiwan Corp.

NIP3 antibody

Western blot analysis of rat thymus tissue lysate. Using BNIP3 polyclonal antibody ( Cat # PAB7995 ) . Rabbit Hu, Ms, Rb, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

NIP3 antibody

NIP3 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

NIP3 antibody

Western blot analysis of BNIP3 expression in HeLa (A); MCF7 (B); SHSY5Y (C); Ramos (D) whole cell lysates. Rabbit Aff - Purified Hu WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NIP3 antibody

Western blot analysis of BNIP3 expression in HeLa (A); MCF7 (B); SHSY5Y (C); Ramos (D) whole cell lysates. Rabbit Aff - Purified Hu WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NIP3 antibody

Western blot analysis of BNIP3 expression in HeLa (A); MCF7 (B); SHSY5Y (C); Ramos (D) whole cell lysates. Rabbit Aff - Purified Hu WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

NIP3 antibody

Monoclonal Anti-BNIP3 antibody produced in mouse Mouse IgG2b ANa40 Purified Hu E, ICC/IF, IP, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

NIP3 antibody

NIP3 Rabbit Aff - Purified Fe, Hu ICC/IF, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

NIP3 antibody

NIP3 Rabbit Aff - Purified Fe, Hu ICC/IF, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

NIP3 antibody

NIP3 Rabbit Aff - Purified Fe, Hu ICC/IF, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

NIP3 antibody

NIP3 Rabbit Aff - Purified Fe, Hu ICC/IF, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

NIP3 antibody

NIP3 Rabbit Aff - Purified Fe, Hu ICC/IF, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

NIP3 antibody

NIP3 Rabbit Aff - Purified Fe, Hu ICC/IF, WB
25 µl / €140.00
  Novus Biologicals Inc.

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