NIPBL antibody

Principal name

NIPBL antibody

Alternative names for NIPBL antibody

IDN3,Delangin, SCC2 homolo, Nipped-B-like protein

SwissProt ID

Q6KC79 (Human), Q6KCD5 (Mouse)

Gene ID

25836 (NIPBL), 71175 (Nipbl)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Can (Canine), Orang-Utan

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of NIPBL antibody

IDN3 (also known as Colon tumor susceptibility 2, DKFZp434L1319, FLJ13648, Mis 4, Scc-2, Delangin and Sister chromatid cohesion protein) is the mammalian homologue of Scc2 in S. cerevisiae and Mis4 in S. pombe. In yeast, this factor was originally identified genetically as being involved in sister chromatid cohesion; subsequent work has determined that it is not involved in cohesion directly but is required for the loading of cohesins onto chromatin during S phase. It shares 37% sequence identity with Nipped-B gene product of Drosophila, which facilitates enhancerpromoter communication of remote enhancers. The Drosophila protein is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. IDN3 is a nuclear protein that is widely expressed and found at the highest levels in heart, skeletal muscle, fetal and adult liver, and fetal and adult kidney. The protein is also found, albeit at lower levels of expression in thymus, placenta, peripheral leukocyte and small intestine. Multiple isoforms of the protein have been reported.

General readings

1. Tonkin,E.T., Wang,T.J., Lisgo,S., Bamshad,M.J. and Strachan,T. (2004) NIPBL, encoding a homolog of fungal Scc2-type sister chromatid cohesion proteins and fly Nipped-B, is mutated in Cornelia de Lange syndrome. Nat. Genet. 36 (6), 636-641.
2. Ben-Asher E, Lancet D. (2004) NIPBL gene responsible for Cornelia de Lange syndrome, a severe developmental disorder.
Isr Med Assoc J. 6(9):571-2.
3. Gillis LA, McCallum J, Kaur M, DeScipio C, Yaeger D, Mariani A, Kline AD, Li HH, Devoto M, Jackson LG, Krantz ID. (2004) NIPBL mutational analysis in 120 individuals with Cornelia de Lange syndrome and evaluation of genotype-phenotype correlations. Am J Hum Genet. 75(4):610-23.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NIPBL (344-356) antibody

WB using Anti-IDN3 antibody shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Primary antibody was used at 1:1,500. Second antibody was a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L]. Goat IgG Aff - Purified Bov, Can, Hu, Ms, Orang-Utan, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NIPBL (Long Form) antibody

NIPBL Rat IgG1 KT54 Aff - Purified Hu, Ms ICC/IF, IP, P, WB
1 mg / €910.00
  Acris Antibodies GmbH

NIPBL (Long Form) antibody

NIPBL Rat IgG1 KT54 Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.2 mg / €450.00
  Acris Antibodies GmbH

NIPBL (Long Form) antibody

NIPBL Rat IgG1 KT54 Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

NIPBL (Short form) antibody

NIPBL Rat IgG2b KT55 Aff - Purified Hu, Ms ICC/IF, IP, P, WB
1 mg / €910.00
  Acris Antibodies GmbH

NIPBL (Short form) antibody

NIPBL Rat IgG2b KT55 Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.2 mg / €450.00
  Acris Antibodies GmbH

NIPBL (Short form) antibody

NIPBL Rat IgG2b KT55 Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

NIPBL Control Peptide

NIPBL
50 µg / €190.00
  Acris Antibodies GmbH

NIPBL (889-902) antibody

NIPBL Goat Aff - Purified Can, Hu C, E, P
0.1 mg / €340.00
  Acris Antibodies GmbH

NIPBL antibody

NIPBL Mouse IgG2a 3B9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NIPBL antibody

NIPBL Rat IgG1 KT54 Hu, Ms C, ICC/IF, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NIPBL antibody

NIPBL Rat IgG2b KT55 Hu, Ms C, ICC/IF, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NIPBL antibody

Western blot using NIPBL polyclonal antibody ( Cat # PAB10226 ) shows detection of a band ~300 kDa correspondingto NIPBL ( arrowhead ) in human kidney ( lane1 ) and human heart ( lane 2 ) whole cell tissue extracts.
Approximately 20 µg of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:500.
Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection. Bov, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

NIPBL antibody

Western Blot: NIPBL Antibody [NB100-93320] - Detection of Human NIPBL by Western Blot of  Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells. Antibody: Affinity purified  rabbit anti-NIPBL antibody NB100-93320 used for IP at 3 mcg/mg  lysate. NIPBL was also immunoprecipitated by rabbit anti-  NIPBL antibody  NB100-93319 , which recognizes an upstream  epitope Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

NIPBL antibody

NIPBL Goat Aff - Purified Ms E, P
0.1 mg / €400.00
  Novus Biologicals Inc.

NIPBL antibody

Immunohistochemistry-Paraffin: Artemis Antibody [NBP1-77975] - Artemis antibody was used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Goat Aff - Purified Hu E, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

NIPBL antibody

Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-85110] Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NIPBL

NIPBL Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NIPBL

NIPBL Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

NIPBL

NIPBL Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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