NIPP-1 antibody

Principal name

NIPP-1 antibody

Alternative names for NIPP-1 antibody

PPP1R8, ARD1, NIPP1, Nuclear inhibitor of protein phosphatase 1

SwissProt ID

Q12972 (Human), Q28147 (Bovin), Q8R3G1 (Mouse)

Gene ID

5511 (PPP1R8)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NIPP-1 antibody

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

General readings

Beullens M & Bollen M The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly. J Biol Chem 277:19855-60 (2002). PubMed PMID: 11909864

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NIPP-1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804811) Mouse IgG2b OTI4D5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIPP-1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804812) Mouse IgG1 OTI4E5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIPP-1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804813) Mouse IgG1 OTI5H9 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIPP-1 antibody

PPP1R8 monoclonal antibody (M21), clone 1G11. Western Blot analysis of PPP1R8 expression in NIH/3T3(Cat # L018V1 ). Mouse IgG1 1G11 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NIPP-1 (C-term) antibody

TA302884 staining (2µg/ml) of H460 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NIPP-1 antibody

Western blot analysis of extracts from Jurkat cells, using PPP1R8 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NIPP-1 antibody

WB Suggested Anti-PPP1R8 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysatePPP1R8 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NIPP-1 antibody

WB Suggested Anti-PPP1R8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: OVCAR-3 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NIPP-1 antibody

WB Suggested Anti-PPP1R8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysatePPP1R8 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NIPP-1 antibody

WB Suggested Anti-PPP1R8 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NIPP-1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R8 (RC210575, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R8. Mouse IgG1 OTI1E2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIPP-1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R8 (RC210575, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R8. Mouse IgG1 OTI1E9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIPP-1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804806) Mouse IgG1 OTI2E12 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIPP-1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R8 (RC210575, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R8. Mouse IgG1 OTI2B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIPP-1 antibody

Detection limit for recombinant GST tagged PPP1R8 is approximately 3ng/ml as a capture antibody. Mouse IgG2a 4B5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NIPP-1 antibody

PPP1R8 polyclonal antibody ( Cat # PAB6447 ) staining ( 2 µg/ml ) of NCI-H460 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NIPP-1 antibody

Western blot analysis of extracts from Jurkat cells, using PPP1R8 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

NIPP-1 antibody

Western blot analysis of extracts from Jurkat cells, using PPP1R8 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

NIPP-1 antibody

Human 293T; WB Suggested Anti-PPP1R8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. PPP1R8 is supported by BioGPS gene expression data to be expressed in HEK293T.; PPP1R8 antibody - middle region (ARP40411_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NIPP-1 antibody

Human Kidney; Rabbit Anti-PPP1R8 Antibody. Catalog Number: ARP40412. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; PPP1R8 antibody - middle region (ARP40412_T100) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

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