NIT1 antibody

Principal name

NIT1 antibody

Alternative names for NIT1 antibody

Nitrilase homolog 1

SwissProt ID

Q7TQ94 (Rat), Q86X76 (Human), Q8VDK1 (Mouse)

Gene ID

4817 (NIT1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), GP (Guinea Pig), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of NIT1 antibody

Kit Component:
- KN211519G1, NIT1 gRNA vector 1 in pCas-Guide vector
- KN211519G2, NIT1 gRNA vector 2 in pCas-Guide vector
- KN211519D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Semba, S. et al., J. Biol. Chem. 281(38): 28244-28253 (2006)
Wang, A.G. et al., Biochem. Biophys. Res. Commun. 345(3): 1022-1032 (2006)
Pekarsky, Y. et al., Proc. Natl. Acad. Sci. U.S.A. 95(15): 8744-8749 (1998)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NIT1 antibody

  anti-NIT1 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501034, Dilution 1:50) Mouse IgG1 OTI4D12 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIT1 antibody

  anti-NIT1 mouse monoclonal antibody, clone OTI3A11 (formerly 3A11)  
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501084, Dilution 1:50) Mouse IgG1 OTI3A11 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

NIT1 antibody

  Rabbit polyclonal Anti-NIT1 Antibody  
WB Suggested Anti-NIT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Small Intestine Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NIT1 antibody

  Rabbit polyclonal Anti-NIT1 Antibody  
WB Suggested Anti-NIT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysateNIT1 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, GP, Hu, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

NIT1 antibody

  Rabbit Polyclonal Anti-NIT1 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human placenta tissue, Human fetal liver tissue, Primary antibody: (NIT1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NIT1 (C-term) antibody

Western blot analysis in ZR-75-1 cell line lysates (35ug/lane) using NIT1 antibody Cat.-No. AP52880PU-N (C-term). This demonstrates this antibody detected the NIT1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

NIT1 - human gene knockout kit via CRISPR

NIT1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NIT1 - human gene knockout kit via CRISPR

NIT1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NIT1 - human gene knockout kit via CRISPR

NIT1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NIT1 - human gene knockout kit via CRISPR

NIT1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NIT1

  Recombinant protein of human nitrilase 1 (NIT1)  
NIT1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

NIT1 (transcript variant 3)

  Purified recombinant protein of Homo sapiens nitrilase 1 (NIT1), transcript variant 3.  
NIT1 Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

NIT1 overexpression lysate

NIT1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

NIT1 overexpression lysate

NIT1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NIT1 (mGFP-tagged) - Human nitrilase 1 (NIT1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, NIT1 (mGFP-tagged) - Human nitrilase 1 (NIT1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, NIT1 (mGFP-tagged) - Human nitrilase 1 (NIT1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, NIT1 (mGFP-tagged) - Human nitrilase 1 (NIT1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, NIT1 (mGFP-tagged) - Human nitrilase 1 (NIT1), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, NIT1 (mGFP-tagged) - Human nitrilase 1 (NIT1), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, NIT1 (mGFP-tagged)-Human nitrilase 1 (NIT1), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, NIT1 (mGFP-tagged)-Human nitrilase 1 (NIT1), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, NIT1 (Myc-DDK tagged) - Human nitrilase 1 (NIT1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, NIT1 (Myc-DDK tagged) - Human nitrilase 1 (NIT1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

Lenti ORF particles, NIT1 (Myc-DDK tagged) - Human nitrilase 1 (NIT1), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, NIT1 (Myc-DDK tagged) - Human nitrilase 1 (NIT1), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Items 1 to 20 of 22 total

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