NIT2 antibody

Principal name

NIT2 antibody

Alternative names for NIT2 antibody

Nitrilase homolog 2

SwissProt ID

Q9NQR4 (Human)

Gene ID

56954 (NIT2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of NIT2 antibody

[FUNCTION] Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle. [SUBUNIT] Homodimer. [SUBCELLULAR LOCATION] Cytoplasm. [TISSUE SPECIFICITY] Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon. [SIMILARITY] Belongs to the UPF0012 family.

General readings

Barqliw K T., et al. (2008) Biochemistry. 47(51):13514-23.
Camarqo A., et al. (2007) Plant Cell. 19(11):3491-503.

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  2. Highly Validated (5)

16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NIT2 antibody

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501081, Dilution 1:50) Mouse IgG2b OTI6H7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIT2 antibody

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501097, Dilution 1:50) Mouse IgG2b OTI3D5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIT2 antibody

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501098, Dilution 1:50) Mouse IgG2b OTI3F3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIT2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501109, Dilution 1:50) Mouse IgG2b OTI2B9 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

NIT2 antibody

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501121, Dilution 1:50) Mouse IgG2a OTI5A3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIT2 antibody

Predicted band size: 31 kDa . Positive control: Mouse liver and kidney tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Mouse liver tissue lysate Lane2: Mouse kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 90 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NIT2 antibody

Predicted band size: 31 kDa . Positive control: Mouse liver and kidney tissue, RAW264.7 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Mouse liver tissue lysate Lane2: Mouse kidney tissue lysate Lane3: RAW264.7 cell lysate Lysates: 40 ug per lane Primary antibody: 1/950 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 90 seconds seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NIT2 antibody

WB Suggested Anti-NIT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NIT2 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501138, Dilution 1:50) Mouse IgG2a OTI1A6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NIT2 antibody

Human Liver; WB Suggested Anti-NIT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Liver; NIT2 antibody - middle region (ARP46292_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NIT2 (1-276, His-tag)

NIT2 Human Purified > 95 % E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

NIT2 (1-276, His-tag)

NIT2 Human Purified > 95 % E. coli
50 µg / €370.00
  Acris Antibodies GmbH

NIT2

NIT2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NIT2

NIT2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

NIT2

NIT2 Human Purified
0.25 mg / €1,140.00
  Novus Biologicals Inc.

NIT2

NIT2 Human Purified
50 µg / €440.00
  Novus Biologicals Inc.

16 Item(s)

per page
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