Nitrotyrosine antibody

Principal name

Nitrotyrosine antibody

Alternative names for Nitrotyrosine antibody

NO-Tyrosine, Nitro-Tyrosine

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), All Species

Available hosts

Mouse, Goat, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Nitrotyrosine antibody

A large number of physiological and pathological events lead to so-called stress conditions to which the organism can adapt within certain limits by regulating the activity of a series of enzymatic cascades. Most stress responses are characterized by an increased generation of free radicals which encompass both reactive oxygen (ROS) and nitrogen species (RNS).
Most of these species react with macromolecules of the organism, i.e. aminoacids, proteins, lipids and DNA, leading to functional alterations which can either participate to adaptation or lead to cell death.
Oxidative stress however, has different functional consequences depending not only upon its intensity and duration, but also upon the nature of the free radicals, ROS or RNS, generated. Interestingly, the nature of these reactive species depends on the ratio between the initially produced ROS, superoxide anion (O2.-), and nitric oxide (NO). Indeed, O2.- very rapidly reacts with NO and therefore, as long as the NO/ O2.-ratio is >/= 1, O2.- will therefore preferentially react with NO rather than with macromolecules, thus generating reactive nitrogen species (RNS): nitrosonium (NO+), N2O3 and peroxinitrite (ONOO-).
These RNS induce posttranslational modifications:
for NO+ and N2O3, S-nitrosation (Cys-SNO) and for ONOO-, tyrosine nitration (Tyr-NO2), methionine sulfoxidation (Met-SOH) and thiol oxidation (RS- S-R). When the O2.-/NO ratio becomes > 1, the O2.- and NO.2 ions and thereafter the OH. radicals, cause irreversible oxidations and peroxidations of macromolecules which generally lead to cell death. (1-4)
The monoclonal antibodies have been selected for their high affinity and specificity towards Tyr-NO2 residues. They do not cross-react with Tyr or Tyr derivatives such as aminotyrosine, chlorotyrosine or phosphotyrosine , neither with nitroTrp which can also be generated in response to peroxynitrite. They also recognize Tyr-NO2 residues in various sequences as shown by their ability to recognize nitrated proteins including albumin, ovalbumin, insulin, hemoglobin, KLH and various cytoplasmic and mitochondrial proteins.

General readings

General / background references:ter Steege JC et al. Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA. Free Radic Biol Med 25:953-63 (1998). PubMed PMID: 9840741
Kooy NW et al. Evidence for in vivo peroxynitrite production in human acute lung injury. Am J Respir Crit Care Med 151:1250-4 (1995). PubMed PMID: 7697261
Beckmann JS et al. Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Biol Chem Hoppe Seyler 375:81-8 (1994). PubMed PMID: 8192861
Beckman JS et al. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci U S A 87:1620-4 (1990). PubMed PMID: 2154753

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nitrotyrosine antibody

Immunoblot Analysis Nitrotyrosine-modified BSA (NOY-BSA) probed with mab NOY-7A5 at 1 µg/ml. Mouse IgG1 7A5 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG2a 39B6 Purified Can, Hu, Ms, Rt C, E, IP, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG2a 39B6 Purified Can, Hu, Ms, Rt C, E, IP, WB
25 µg / €240.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Legend A. Western blot using 3-nitrotyrosine monoclonal antibody on 40 µg mouse brain lysate (Lane 1) and 40 µg rat brain lysate (Lane 2). Antibody used at a dilution of 1µg/ml, detected with Supersignal West Pico Substrate -30 second exposure. Legend B. Same experiment blocked with buffer containing 1 mM 3-nitrosine. Mouse IgG1 2A12 Purified C, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG1 11C2 Supernatant C, E, ICC/IF, WB
1 ml / €400.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Immunohistochemistry staining (frozen sections) of Human tumoral colon epithelium with anti-Nitrotyrosine antibody (EM-30). Mouse IgG1 EM-30 Aff - Purified All Species C, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11 (Cat. # AM26352PU-N). Anti-nitrotyrosine at 2µg/ml (1h, RT) Mouse IgG2b HM.11 Biotin C, E, P, WB
50 µg / €340.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone   HM.11 (Cat. # AM26352PU-N). Anti-nitrotyrosine at 2µg/ml (1h, RT). Mouse IgG2b HM.11 Purified C, E, P, WB
0.1 mg / €410.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG1 6B2-3G2 Purified Hu E, IP, WB

Regular Price: 0.1 mg / €280.00

Special Price: 0.1 mg / €196.00

  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG1 6B2-3G2 Purified Hu E, IP, WB

Regular Price: 50 µg / €170.00

Special Price: 50 µg / €119.00

  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG2b 2E11-3D3 Purified Hu E, IP, WB

Regular Price: 0.1 mg / €280.00

Special Price: 0.1 mg / €196.00

  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG2b 2E11-3D3 Purified Hu E, IP, WB

Regular Price: 50 µg / €170.00

Special Price: 50 µg / €119.00

  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG2b HM11 Biotin Hu C, E, P, WB
0.5 ml / €510.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG2b HM11 Purified Hu C, E, P, WB
1 ml / €620.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Goat Serum E, WB
1 ml / €420.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Rabbit Serum E, IP, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG1 1F1 E, IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Nitrotyrosine antibody

Nitrotyrosine Mouse IgG1 2A12 WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Nitrotyrosine antibody

Nitrotyrosine Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

Nitrotyrosine antibody

Nitrotyrosine Rabbit IgG Purified E, ICC/IF
0.1 ml / €730.00
  Abnova Taiwan Corp.

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