NKX2-2 antibody

Principal name

NKX2-2 antibody

Alternative names for NKX2-2 antibody

NKX2.2, NKX2B, Homeobox protein Nkx-2.2, NK2 transcription factor related, locus 2

SwissProt ID

O95096 (Human), P42586 (Mouse), P43697 (Mesau)

Gene ID

4821 (NKX2-2), 18088 (Nkx2-2), 3482154 (acpP)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of NKX2-2 antibody

The NKX families of homeobox genes are known to act as intermediaries in the neural response to sonic hedgehog signaling during central nervous system development. NKX2.2 is a member of this family of transcription factors and is necessary for neuroendocrine differentiation in the central nervous system and pancreas (Sander M, 2000). This protein was recently identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It also acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors (Yoshida A, 2012). NKX2.2 preferentially binds the promoter of the αcell-specific factor Arx in βcells when the promoter is in a hypermethylated state. Disruption of the interaction between NKX2.2 and Grgs in mice leads to ectopic expression of Arx in βcells and is sufficient to cause β-to-α-cell reprogramming during embryonic development and in the adult. These combined in vitro and in vivo studies elucidate how cell-specific DNA modifications and transcriptional regulatory events come together to establish and maintain islet βcell identity (Papizan JB, 2011).

General readings

Wang, Y.C. et al., J. Surg. Res. 163(1): 47-51 (2010)
Wang, Y.C. et al., Endocr. Relat. Cancer 16(1): 267-279 (2009)
Chen, M. et al., J. Biol. Chem. 284(3): 1484-1494 (2009)
Owen, L.A. et al., PLoS ONE 3 (4): e1965 (2008)
Pauls, S. et al., Dev. Biol. 304(2): 875-890 (2007)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NKX2-2 antibody

Formalin-Fixed, Paraffin-Embedded Human Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM32850PU (Clone NX2/294). Mouse IgG2b NX2/294 Purified Hu C, E, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

NKX2-2 antibody

Formalin-Fixed, Paraffin-Embedded Human Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM32850PU (Clone NX2/294). Mouse IgG2b NX2/294 Purified Hu C, E, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

NKX2-2 antibody

Formalin-Fixed, Paraffin-Embedded Human Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM32850PU (Clone NX2/294). Mouse IgG2b NX2/294 Purified Hu C, E, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

NKX2-2 antibody

Formalin-paraffin Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM50148PU (Clone SPM564). Mouse IgG2b SPM564 Purified Chk, Hu, Ms, Rt F, ICC/IF, P
0.2 mg / €390.00
  Acris Antibodies GmbH

NKX2-2 antibody

Formalin-paraffin Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM50148PU (Clone SPM564). Mouse IgG2b SPM564 Purified Chk, Hu, Ms, Rt F, ICC/IF, P
0.1 mg / €320.00
  Acris Antibodies GmbH

NKX2-2 antibody

Formalin-paraffin Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM50148PU (Clone SPM564). Mouse IgG2b SPM564 Purified Chk, Hu, Ms, Rt F, ICC/IF, P
20 µg / €160.00
  Acris Antibodies GmbH

NKX2-2 (Center) antibody

Western blot analysis of NKX2-2 in rat kidney tissue lysate with NKX2-2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NKX2-2 antibody

Host: Rabbit; Target Name: NKX2-2; Sample Tissue: Jurkat Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

NKX2-2 antibody

WB Suggested Anti-NKX2-2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Spleen Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NKX2-2 antibody

WB Suggested Anti-NKX2-2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NKX2-2 antibody

WB Suggested Anti-NKX2-2 Antibody Titration: 0.125 ug/ml; ELISA Titer: 1:1562500; Positive Control: SP2/0 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NKX2-2 antibody

Western blot analysis using NKX2.2 antibody Cat.-No AM06771PU-N against human NKX2.2 recombinant protein (Expected MW is 22 kDa) Mouse IgG1 3E4 Purified Hu E, F, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

NKX2-2 (Center) antibody

Western blot analysis in 293 cell line lysates (35ug/lane) using NKX2-2 antibody Cat.-No. AP52892PU-N. This demonstrates this antibody detected the NKX2-2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NKX2-2 (Center) Control Peptide

Western blot analysis in rat kidney tissue lysate with NKX2-2 antibody Cat.-No. AP55626PU-N at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
50 µg / €160.00
  Acris Antibodies GmbH

NKX2-2 antibody

NKX2-2 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

NKX2-2 antibody

Human; . Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage CellsPrimary. Dilution:. IHC-PZ 1:500, IHC-P 1:1000; NKX2-2 antibody - N-terminal region (ARP32338_P050) in Human cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

NKX2-2 antibody

Mouse SP2/0; WB Suggested Anti-NKX2-2 Antibody Titration: 0.125ug/ml. ELISA Titer: 1:1562500. Positive Control: SP2/0 cell lysate; NKX2-2 antibody - N-terminal region (ARP37110_P050) in Mouse SP2/0 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

NKX2-2 antibody

Hum. Fetal Brain; NKX2-2 antibody - N-terminal region (ARP38167_P050) in Hum. Fetal Brain cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

NKX2-2 antibody

Immunohistochemistry-Paraffin: Nkx2.2 Antibody [NBP1-82554] - Immunohistochemical staining of human pancreas shows strong nuclear positivity in islet cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NKX2-2 (full length, N-term HIS tag)

NKX2-2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

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