NKX2-2 antibody

Principal name

NKX2-2 antibody

Alternative names for NKX2-2 antibody

NKX2.2, NKX2B, Homeobox protein Nkx-2.2, NK2 transcription factor related, locus 2

SwissProt ID

O95096 (Human), P42586 (Mouse), P43697 (Mesau)

Gene ID

18088 (Nkx2-2), 4821 (NKX2-2)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of NKX2-2 antibody

The NKX families of homeobox genes are known to act as intermediaries in the neural response to sonic hedgehog signaling during central nervous system development. NKX2.2 is a member of this family of transcription factors and is necessary for neuroendocrine differentiation in the central nervous system and pancreas (Sander M, 2000). This protein was recently identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It also acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors (Yoshida A, 2012). NKX2.2 preferentially binds the promoter of the αcell-specific factor Arx in βcells when the promoter is in a hypermethylated state. Disruption of the interaction between NKX2.2 and Grgs in mice leads to ectopic expression of Arx in βcells and is sufficient to cause β-to-α-cell reprogramming during embryonic development and in the adult. These combined in vitro and in vivo studies elucidate how cell-specific DNA modifications and transcriptional regulatory events come together to establish and maintain islet βcell identity (Papizan JB, 2011).

General readings

Wang, Y.C. et al., J. Surg. Res. 163(1): 47-51 (2010)
Wang, Y.C. et al., Endocr. Relat. Cancer 16(1): 267-279 (2009)
Chen, M. et al., J. Biol. Chem. 284(3): 1484-1494 (2009)
Owen, L.A. et al., PLoS ONE 3 (4): e1965 (2008)
Pauls, S. et al., Dev. Biol. 304(2): 875-890 (2007)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NKX2-2 antibody

Formalin-Paraffin Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM50148PU (Clone SPM564). Mouse IgG2b SPM564 Purified Chk, Hu, Ms, Rt F, ICC/IF, P
0.2 mg / €410.00
  Acris Antibodies GmbH

NKX2-2 antibody

Formalin-Paraffin Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM50148PU (Clone SPM564). Mouse IgG2b SPM564 Purified Chk, Hu, Ms, Rt F, ICC/IF, P
0.1 mg / €320.00
  Acris Antibodies GmbH

NKX2-2 antibody

Formalin-Paraffin Ewing’s sarcoma stained with NKX2.2 Antibody Cat.-No AM50148PU (Clone SPM564). Mouse IgG2b SPM564 Purified Chk, Hu, Ms, Rt F, ICC/IF, P
20 µg / €180.00
  Acris Antibodies GmbH

NKX2-2 (Center) antibody

  Rabbit Polyclonal NKX2-2 Antibody  
Western blot analysis of NKX2-2 in rat kidney tissue lysate with NKX2-2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NKX2-2 antibody

  Rabbit Polyclonal Anti-NKX2  
Host: Rabbit; Target Name: NKX2-2; Sample Tissue: Jurkat Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

NKX2-2 antibody

  Rabbit Polyclonal Anti-NKX2 Antibody  
WB Suggested Anti-NKX2-2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Spleen Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NKX2-2 antibody

  Rabbit Polyclonal Anti-NKX2-2 Antibody  
WB Suggested Anti-NKX2-2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NKX2-2 antibody

  Rabbit Polyclonal Anti-NKX2-2 Antibody  
WB Suggested Anti-NKX2-2 Antibody Titration: 0.125 ug/ml; ELISA Titer: 1:1562500; Positive Control: SP2/0 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NKX2-2 antibody

Western blot analysis using NKX2.2 antibody Cat.-No AM06771PU-N against human NKX2.2 recombinant protein (Expected MW is 22 kDa) Mouse IgG1 3E4 Purified Hu E, F, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

NKX2-2 (Center) antibody

Western blot analysis in 293 cell line lysates (35ug/lane) using NKX2-2 antibody Cat.-No. AP52892PU-N. This demonstrates this antibody detected the NKX2-2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Nkx2 - mouse gene knockout kit via CRISPR

Nkx2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Nkx2 - mouse gene knockout kit via CRISPR

Nkx2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nkx2 - mouse gene knockout kit via CRISPR

Nkx2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nkx2 - mouse gene knockout kit via CRISPR

Nkx2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NKX2-2 (full length, N-term HIS tag)

  Recombinant protein of human NK2 homeobox 2 (NKX2-2) , full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
NKX2-2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

NKX2 overexpression lysate

NKX2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NKX2 (mGFP-tagged) - Human NK2 homeobox 2 (NKX2-2), 200 uL, >10^7 TU/mL

Lenti ORF particles, NKX2 (mGFP-tagged) - Human NK2 homeobox 2 (NKX2-2), 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, NKX2 (Myc-DDK tagged) - Human NK2 homeobox 2 (NKX2-2) , 200 uL, >10^7 TU/mL

Lenti ORF particles, NKX2 (Myc-DDK tagged) - Human NK2 homeobox 2 (NKX2-2) , 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nkx2 (GFP-tagged) - Mouse NK2 transcription factor related, locus 2 (Drosophila) (Nkx2-2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Nkx2 (GFP-tagged) - Mouse NK2 transcription factor related, locus 2 (Drosophila) (Nkx2-2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Nkx2 (GFP-tagged) - Mouse NK2 transcription factor related, locus 2 (Drosophila) (Nkx2-2), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Nkx2 (GFP-tagged) - Mouse NK2 transcription factor related, locus 2 (Drosophila) (Nkx2-2), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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