NKX2-5 antibody

Principal name

NKX2-5 antibody

Alternative names for NKX2-5 antibody

CSX, NKX2.5, NKX2E, Homeobox protein Nkx-2.5, Homeobox protein NK-2 homolog E, Cardiac-specific homeobox, Homeobox protein CSX

SwissProt ID

O35767 (Rat), P42582 (Mouse), P52952 (Human), Q90788 (Chick)

Gene ID

1482 (NKX2-5), 18091 (Nkx2-5)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rb (Rabbit), Rt (Rat), Fe (Feline), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P)

Background of NKX2-5 antibody

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM].

General readings

Briggs LE, Takeda M, Cuadra AE, Wakimoto H, Marks MH, Walker AJ, Seki T, Oh SP, Lu JT, Sumners C, Raizada MK, Horikoshi N, Weinberg EO, Yasui K, Ikeda Y, Chien KR, Kasahara H.
Perinatal loss of Nkx2-5 results in rapid conduction and contraction defects.
Circulation research 2008 Sep 103 (6): 580-90.
PMID: 18689573

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NKX2-5 antibody

Sample(30 ug whole cell lysate). A:H1299. B:Raji . 10% SDS PAGE. TA308654 diluted at 1:1000 Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NKX2-5 antibody

NKX2-5 monoclonal antibody (M01), clone 1E4-G5. Western Blot analysis of NKX2-5 expression in human thyroid(diffuse hyperplasia). Mouse IgG1 1E4-G5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NKX2-5 antibody

TA305653 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NKX2-5 antibody

Western blot analysis of extracts of various cell lines, using NKX2-5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NKX2-5 antibody

WB Suggested Anti-Nkx2-5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Mouse Thymus Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NKX2-5 antibody

Western blot analysis using NKX2.5 mouse mAb against full-length NKX2.5 (aa1-324)-hIgGFc transfected HEK293 cell lysate (1). Mouse IgG1 2E1 Aff - Purified Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

NKX2-5 antibody

NKX2-5 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

NKX2-5 antibody

Western Blot analysis of NKX2-5 expression in transfected 293T cell line (H00001482-T01) by NKX2-5 MaxPab polyclonal antibody.

Lane 1: NKX2-5 transfected lysate(34.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NKX2-5 antibody

Detection limit for recombinant GST tagged NKX2-5 is approximately 0.1ng/ml as a capture antibody. Mouse IgG1 S1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NKX2-5 antibody

Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M03), clone 3A7.

Lane 1: NKX2-5 transfected lysate(34.9 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 3A7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NKX2-5 antibody

NKX2-5 Mouse IgG2a 3C1 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

NKX2-5 antibody

NKX2-5 Mouse IgG2b 4B11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NKX2-5 antibody

Western Blot analysis of NKX2-5 expression in transfected 293T cell line by NKX2-5 monoclonal antibody (M08), clone 3E3.

Lane 1: NKX2-5 transfected lysate(34.92 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 3E3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NKX2-5 antibody

Human MCF-7; WB Suggested Anti-Nkx2-5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate; Nkx2-5 antibody - N-terminal region (ARP31575_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NKX2-5 antibody

Mouse Thymus; WB Suggested Anti-Nkx2-5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Mouse Thymus; Nkx2-5 antibody - N-terminal region (ARP36946_P050) in Mouse Thymus cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NKX2-5 antibody

Western Blot: Nkx2.5 Antibody (2E1) [NBP1-51579] - Western blot analysis using NKX2.5 mouse mAb against full-length NKX2.5 (aa1-324)-hIgGFc transfected HEK293 cell lysate (1). Mouse IgG1 2E1 Purified Hu E, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

NKX2-5 antibody

NKX2-5 Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NKX2-5

NKX2-5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NKX2-5

NKX2-5 Human
2 µg / €300.00
  Abnova Taiwan Corp.

NKX2-5

NKX2-5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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