NLK antibody

Principal name

NLK antibody

Alternative names for NLK antibody

LAK1, Nemo-like kinase, Serine/threonine protein kinase NLK

SwissProt ID

O54949 (Mouse), Q9UBE8 (Human)

Gene ID

51701 (NLK), 18099 (Nlk)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NLK antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NLK antibody

  Rabbit Polyclonal antibody to NLK (nemo-like kinase)  
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA309011 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NLK antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K7 and NLK HeLa cells were stained with anti-MAP3K7 rabbit purified polyclonal 1:1200 and anti-NLK mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 2B11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NLK antibody

  Mouse monoclonal NLK Antibody  
NLK Antibody(Cat. #TA324850) western blot analysis in SiHa,Hela cell line lysates (35ug/lane).This demonstrates the NLK antibody detected the NLK protein (arrow). Mouse IgG2a 1146CT24.2.1 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NLK antibody

  Rabbit anti-NLK Polyclonal Antibody  
Western blot analysis of extracts of HeLa cell line, using NLK antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NLK antibody

  Rabbit Polyclonal Anti-NLK Antibody - middle region  
WB Suggested Anti-NLK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NLK (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NLK antibody

NLK Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

NLK antibody

NLK Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

NLK antibody

Detection limit for recombinant GST tagged NLK is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 1C1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NLK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with NLK polyclonal antibody ( Cat # PAB2944 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

NLK antibody

NLK Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

NLK - human gene knockout kit via CRISPR

NLK - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NLK - human gene knockout kit via CRISPR

NLK - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NLK - human gene knockout kit via CRISPR

NLK - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NLK - human gene knockout kit via CRISPR

NLK - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nlk - mouse gene knockout kit via CRISPR

Nlk - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Nlk - mouse gene knockout kit via CRISPR

Nlk - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nlk - mouse gene knockout kit via CRISPR

Nlk - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nlk - mouse gene knockout kit via CRISPR

Nlk - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NLK

  Recombinant protein of human nemo-like kinase (NLK)  
NLK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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