NLRC4 antibody

Principal name

NLRC4 antibody

Alternative names for NLRC4 antibody

CARD12, CLAN, CLAN1, IPAF, NLR family CARD domain-containing protein 4

SwissProt ID

Q9NPP4 (Human)

Gene ID

58484 (NLRC4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP)

Background of NLRC4 antibody

Ipaf (also known as Clan/CARD12) is a CARD domain containing protein. CARD (caspase-associated recruitment domain) proteins are key regulators of cell death, cell survival and cytokine production (reviewed in Damiano and Reed, 2004). In general CARD proteins are implicated in host defense against infection, environmental stress or cellular damage. CARD domains are found in the N-terminal pro-domains of certain caspases, a family of apoptotic and pro-inflammatory proteases, as well as in a diversity of other proteins including Ipaf/Clan/CARD12. CARD domains are homotypic protein interaction motifs that enable networks of proteins to communicate via CARD-CARD interactions. There are at least three major signaling pathways in which CARD proteins act: (1) Regulation of caspase activation in the context of apoptosis (2) Regulation of caspase activation in the context of inflammation (3) Regaultion of NF-kB activation in the context of innate or adaptive immune responses. As there is significant crosstalk between pathways that lead to caspase-mediated apoptosis or inflammation and pathways that result in NF-kB activation, it is logical that similar protein modules such as CARD domains are found repeatedly in proteins from all three pathways. Ipaf plays a role in regulating caspase-1 activity, which in turn mediates the maturation of inflammatory cytokines IL-1b and IL-18 (reviewed in Lu et al, 2005). In transfected cells, Ipaf has been shown to directly interact with procaspase-1 and induce proteolytic activation of procaspase-1 in transfected cells. On the flip side, macrophages from IPAF deficient mice failed to activate caspase-1 in response to Salmonella typhimurium infection underscoring the importance of IPAF in vivo. IPAF also interact with the pro-apoptotic adaptor protein ASC and co-expression of IPAF with ASC has been shown to induce NF-kB activation and apoptosis.

General readings

Damiano J.S., Stehlik C., Pio F., Godzik A., and Reed J.C. Genomics 2001 Jul;75(1-3):77-83

16 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NLRC4 antibody

Western blot analysis of Ipaf in human PBL lysate with Ipaf antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) ug/mL, respectively. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NLRC4 antibody

WB Suggested Anti-NLRC4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NLRC4 antibody

Western Blot: CARD12 Antibody [TA336660] - Western Blot analysis of CLAN in lung lysate with anti-CLAN at a 1:500 dilution. Detects CLAN-A and CLAN-B isoforms. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NLRC4 antibody

Immunohistochemistry: CARD12 Antibody [TA336852] - IHC analysis of CARD12 in mouse lung using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NLRC4 antibody

Immunohistochemistry: CARD12 Antibody [TA336853] - IHC analysis of CARD12 in mouse lung using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NLRC4 antibody

AP07715PU-N NLRC4 antibody satining of Formalin-Fixed Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NLRC4 Control Peptide

NLRC4
50 µg / €160.00
  Acris Antibodies GmbH

NLRC4 antibody

NLRC4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NLRC4 antibody

NLRC4 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

NLRC4 antibody

NLRC4 Rabbit Hu, Ms C, IP, P
50 µl / €470.00
  Abnova Taiwan Corp.

NLRC4 antibody

Immunocytochemistry of NLRC4 in THP-1 cells with NLRC4 polyclonal antibody ( Cat # PAB12895 ) at 10 µg/ml. Rabbit Hu ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

NLRC4 antibody

Human Smooth Muscle; NLRC4 antibody - C-terminal region (AVARP00019_P050) in Human Smooth Muscle cells using Immunohistochemistry Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

NLRC4 antibody

NLRC4 Rabbit IgG Serum Hu, Ms C, IP, P, WB
50 µl / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NLRC4

NLRC4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NLRC4

NLRC4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NLRC4 overexpression lysate

NLRC4 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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