NMDAR2B antibody

Principal name

NMDAR2B antibody

Alternative names for NMDAR2B antibody

GRIN2B, NMDA Receptor 2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3

SwissProt ID

Q00960 (Rat), Q01097 (Mouse), Q13224 (Human)

Gene ID

2904 (GRIN2B), 24410 (Grin2b)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), Xen (Xenopus), African clawed frog, Fi (Fish), Prim (Primate)

Available hosts

Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of NMDAR2B antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces.
There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2).
NR2B containing receptors have been implicated in synaptic plasticity, memory formation and pain modulation (3). Studies suggest that the NR2B subunit of glutamate receptors may be potential targets for relieving pain; NR2B may be a probable target for anti-nociceptive drugs, and may also be useful as analgesics (4).

General readings

1. Clayton, D.A., Mesches, M.H., Alvarez, E., Bickford, P.C. and Browning, M.D. "A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial
learning in the Fischer 344 rat," J. Neurosci. 22 (2002) 3628 - 3637.
2. Grosshans, D.R., Clayton, D.A., Coultrap, S.J. and Browning, M.D. "LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1," Nature Neurosci. 5 (2002)27 - 33.
3. Loftis, J.M. and Janowsky, A. "The N-methyl-D-aspartate receptor subunit NR2B: localization, functional properties, regulation, and clinical implications," Pharmacol. Ther. 97 (2003)55 - 85.
4. Nakazawa, T., Komai, S., Tezuka, T., Hisatsune, C., Umemori, H., Semba, K., Mishina, M., Manabe, T. and Yamamoto, T.
"Characterization of Fyn-mediated tryosine phosphorylation
sites on the NR2B subunit of the N-methyl-D-aspartate receptor," J. Biol. Chem. 276 (2001) 693 - 699.
5. Snell, L.D., Nunley, K.R., Lickteig, R.L., Browning, M.D., Tabakoff, B., and Hoffman, P.L."Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion," Mol. Brain Res. 40 (1996) 71 - 78.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NMDAR2B (C-term) antibody

Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDA receptor. Rabbit IgG Aff - Purified Hu, Ms, Rt C, IP, WB
10 µg / €325.00
  OriGene Technologies, Inc.

NMDAR2B pTyr1252 antibody

Figure 2. NR2B Tyr1252 and PSD95 Double-Label Immunostaining of 14 DIV Cortical Neurons. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt, Ze C, ICC/IF, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

NMDAR2B pTyr1336 antibody

Immunostaining of mouse dentate gyrus 48 hr post TMT treatment showing NR2B when phosphorylated at Tyr1336 in green and nuclei in blue. Photo Courtesy of Rob Wine. Rabbit IgG Aff - Purified Hu, Ms, non-Human Primates, Rt C, ICC/IF, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

NMDAR2B pTyr1472 antibody

Figure 1. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1472 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt, Ze WB
0.1 ml / €380.00
  Acris Antibodies GmbH

NMDAR2B antibody

Western blot analysis of rat brain lysates: 1. Anti-NMDA Receptor 2B (GluN2B) (extracellular) antibody, (1:600). 2. Anti-NMDA Receptor 2B (GluN2B) (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

NMDAR2B antibody

immunoblot against rat brain membranes (RBM) and neuronal lysates from NR2B knockout (KO) and heterozygote (Het) mice. Samples courtesy of Ben Hall and Anirvan Ghosh (UCSD Mouse IgG2b N59/36 Supernatant Hu, Ms, Rt C, ICC/IF, IP, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

NMDAR2B antibody

Immunoblot against rat brain membranes (RBM) and neuronal lysates from NR2B knockout (KO) and heterozygote (Het) mice. Samples courtesy of Ben Hall and Anirvan Ghosh (UCSD). Mouse IgG2b N59/36 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

NMDAR2B antibody

TA303120 (2µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NMDAR2B (C-term) antibody

Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDA receptor. Rabbit IgG Serum Hu, Ms, Rt WB
50 µl / €325.00
  OriGene Technologies, Inc.

NMDAR2B (N-term) antibody

Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDA receptor. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NMDAR2B (N-term) antibody

Western blot analysis of NR2B in rat brain membrane lysates using a 1:1000 dilution of TA309447. Mouse IgG2a S59-20 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NMDAR2B (N-term) antibody

Western blot analysis of NR2B in rat brain membrane lysates using a 1:1000 dilution of TA309448. Mouse IgG2b S59-36 Purified Hu, Ms, Rt C, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NMDAR2B antibody

Western blot analysis of extracts from mouse brain cells, using GRIN2B(Ab-1303) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NMDAR2B antibody

Predicted band size: 166 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NMDAR2B antibody

Western blot analysis of NMDAR2B expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NMDAR2B antibody

Western blot analysis of NMDAR2B expression in A549 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NMDAR2B antibody

Top middle: immunoblots on brain membranes prepared from whole rat (RBM, also top right figure) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N59/20 (left) or N52A/42 (right) TC supe.Lower middle: immunoblot against rat brain membranes (RBM) and neuronal lysates from NR2B knockout (KO) and heterozygote (Het) mice. Samples courtesy of Ben Hall and Anirvan Ghosh (UCSD). Mouse IgG2a N59/20 Supernatant Hu, Ms, Rt C, ICC/IF, IP, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

NMDAR2B antibody

Top middle: immunoblots on brain membranes prepared from whole rat (RBM, also top right figure) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N59/20 (left) or N52A/42 (right) TC supe.Lower middle: immunoblot against rat brain membranes (RBM) and neuronal lysates from NR2B knockout (KO) and heterozygote (Het) mice. Samples courtesy of Ben Hall and Anirvan Ghosh (UCSD). Mouse IgG2a N59/20 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

NMDAR2B pSer1480 antibody

Figure 1. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser1480 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser1480 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt, Xen WB
0.1 ml / €380.00
  Acris Antibodies GmbH

NMDAR2B pTyr1474 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using NMDAR2B (phospho-Tyr1474) antibody Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
0.1 ml / €360.00
  Acris Antibodies GmbH

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