NNMT antibody

Principal name

NNMT antibody

Alternative names for NNMT antibody

Nicotinamide N-methyltransferase

SwissProt ID

O55239 (Mouse), P40261 (Human)

Gene ID

4837 (NNMT)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Por (Porcine), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NNMT antibody

NNMT(Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

General readings

Parsons RB., et al. (2002). J Neuropathol Exp Neurol. 61(2):111-24.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NNMT antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502624) Mouse IgG2b OTI3D8 Purified Hu, Ms F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NNMT antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502470) Mouse IgG1 OTI1A1 Purified Can, Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NNMT antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502521) Mouse IgG1 OTI3E3 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NNMT antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502522) Mouse IgG1 OTI2G8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NNMT antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502538) Mouse IgG2b OTI2A3 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NNMT antibody

Western blot analysis of NNMT (arrow) using rabbit polyclonal NNMT Antibody (Center)(Cat# TA302022). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NNMT gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NNMT antibody

TA341383 (0.3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NNMT antibody

TA341384 (0.1µg/ml) staining of A549 (A) and HeLA (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NNMT antibody

NNMT polyclonal antibody (A01), Lot # CIL0051213QCS1 Western Blot analysis of NNMT expression in A-431 ( Cat # L015V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NNMT antibody

NNMT MaxPab rabbit polyclonal antibody. Western Blot analysis of NNMT expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NNMT antibody

Western Blot analysis of NNMT expression in transfected 293T cell line by NNMT monoclonal antibody (M03), clone 2F2.

Lane 1: NNMT transfected lysate(29.6 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 2F2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NNMT antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with NNMT polyclonal antibody ( Cat # PAB2193 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

NNMT antibody

Human HepG2; WB Suggested Anti-NNMT Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; NNMT antibody - N-terminal region (ARP42281_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

NNMT antibody

NNMT Rabbit IgG Hu WB
0.1 ml / €340.00
  Novus Biologicals Inc.

NNMT antibody

Western Blot: NNMT Antibody [NBP1-55398] - This Anti-NNMT antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 2.5ug/ml. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NNMT

NNMT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NNMT (1-264, His-tag)

NNMT Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

NNMT (1-264, His-tag)

NNMT Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

NNMT

NNMT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NNMT

NNMT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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