NO66 antibody

Principal name

NO66 antibody

Alternative names for NO66 antibody

Nucleolar protein 66, hsNO66, C14orf169

SwissProt ID

Q9H6W3 (Human), Q9JJF3 (Mouse)

Gene ID

79697 (C14orf169), 71952 (2410016O06Rik)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NO66 antibody

C14orf169 is the human homolog of Xenopus xNO66. xNO66 is a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. It is a novel, ubiquitous protein, which in most cell types appears to be a constitutive nucleolar component. Immunolocalization studies have revealed that this protein is highly conserved during evolution and shows in most cells analyzed a dual localization pattern, i.e., a strong enrichment in the granular part of nucleoli and in distinct nucleoplasmic entities. Colocalizations with proteins Ki-7, HP1alpha, and PCNA, respectively, have further shown that the staining pattern of xNO66 overlaps with certain clusters of late replicating chromatin. Biochemical experiments have revealed that protein NO66 cofractionates with large preribosomal particles but is absent from cytoplasmic ribosomes. It is proposed that in addition to its role in ribosome biogenesis protein xNO66 has functions in the replication or remodeling of certain heterochromatic regions.

General readings

Eilbracht J, Reichenzeller M, Hergt M, Schnolzer M, Heid H, Stohr M, Franke WW, Schmidt-Zachmann MS.
NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin.
Mol Biol Cell. 2004 Apr;15(4):1816-32. Epub 2004 Jan 23.
PMID: 14742713

9 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NO66 antibody

TA303194 (0.3µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NO66 antibody

WB Suggested Anti-C14ORF169 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysateC14ORF169 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Hu, Rb WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NO66 antibody

NO66 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

NO66 antibody

C14orf169 polyclonal antibody ( Cat # PAB7527 ) ( 0.3 µg/ml ) staining of A-431 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NO66 antibody

Human HepG2; WB Suggested Anti-C14ORF169 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate. C14ORF169 is supported by BioGPS gene expression data to be expressed in HepG2.; C14ORF169 antibody - N-terminal region (ARP33580_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Can, Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

NO66 antibody

NB100-93381 (0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NO66

NO66 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

C14orf169 293T Cell Transient Overexpression Lysate(Denatured)

C14orf169 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

C14orf169 overexpression lysate

C14orf169 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

9 Item(s)

per page
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