NO66 antibody

Principal name

NO66 antibody

Alternative names for NO66 antibody

Nucleolar protein 66, hsNO66, C14orf169

SwissProt ID

Q9H6W3 (Human), Q9JJF3 (Mouse)

Gene ID

79697 (C14orf169), 71952 (2410016O06Rik)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NO66 antibody

C14orf169 is the human homolog of Xenopus xNO66. xNO66 is a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. It is a novel, ubiquitous protein, which in most cell types appears to be a constitutive nucleolar component. Immunolocalization studies have revealed that this protein is highly conserved during evolution and shows in most cells analyzed a dual localization pattern, i.e., a strong enrichment in the granular part of nucleoli and in distinct nucleoplasmic entities. Colocalizations with proteins Ki-7, HP1alpha, and PCNA, respectively, have further shown that the staining pattern of xNO66 overlaps with certain clusters of late replicating chromatin. Biochemical experiments have revealed that protein NO66 cofractionates with large preribosomal particles but is absent from cytoplasmic ribosomes. It is proposed that in addition to its role in ribosome biogenesis protein xNO66 has functions in the replication or remodeling of certain heterochromatic regions.

General readings

Eilbracht J, Reichenzeller M, Hergt M, Schnolzer M, Heid H, Stohr M, Franke WW, Schmidt-Zachmann MS.
NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin.
Mol Biol Cell. 2004 Apr;15(4):1816-32. Epub 2004 Jan 23.
PMID: 14742713

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NO66 antibody

  Goat Polyclonal Antibody against C14orf169  
TA303194 (0.3µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NO66 antibody

  Rabbit Polyclonal Anti-C14ORF169 Antibody  
WB Suggested Anti-C14ORF169 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysateC14ORF169 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Hu, Rb WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

2410016O06Rik - mouse gene knockout kit via CRISPR

2410016O06Rik - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

2410016O06Rik - mouse gene knockout kit via CRISPR

2410016O06Rik - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

2410016O06Rik - mouse gene knockout kit via CRISPR

2410016O06Rik - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

2410016O06Rik - mouse gene knockout kit via CRISPR

2410016O06Rik - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

C14orf169 - human gene knockout kit via CRISPR

C14orf169 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

C14orf169 - human gene knockout kit via CRISPR

C14orf169 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

C14orf169 - human gene knockout kit via CRISPR

C14orf169 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

C14orf169 - human gene knockout kit via CRISPR

C14orf169 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

C14orf169 overexpression lysate

C14orf169 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

11 Item(s)

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