NOB1 antibody

Principal name

NOB1 antibody

Alternative names for NOB1 antibody

ART4, NOB1P, PSMD8BP1, MSTP158, ART-4, RNA-binding protein NOB1,Phosphorylation regulatory protein HP-10

SwissProt ID

Q9ULX3 (Human)

Gene ID

28987 (NOB1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB)

Background of NOB1 antibody

In yeast, over 200 protein and R cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rR transcript into the mature 18S, 5.8S, and 25S rRs, folding of the rR, and binding of ribosomal proteins and 5S R. Nob1 is involved in pre-rR processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rR intermediate at cleavage site D to produce the mature 18S rR (Laman and Karbstein, 2009 [PubMed 19706509]).[supplied by OMIM, Nov 2010]. Transcript Variant: This variant (2) lacks an interl exon, compared to variant 1. This variant is represented as non-coding due to the presence of an upstream ORF that is predicted to interfere with translation of the longest ORF; translation of the upstream ORF renders the transcript a candidate for nonsense-mediated mR decay (NMD). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additiol publications. ##Evidence-Data-START## Transcript exon combition :: BG386829.1 [ECO:0000332] Rseq introns :: mixed/partial sample support ERS025081, ERS025082 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

General readings

Zhang Y., et al. (2005) Kidney Int. 79:1138-1148Daub H., et al. (2008) Mol. Cell. 31:438-448

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NOB1 antibody

  Rabbit Polyclonal Anti-NOB1 Antibody  
WB Suggested Anti-NOB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NOB1 antibody

  Rabbit Polyclonal Anti-NOB1 Antibody  
WB Suggested Anti-NOB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NOB1 antibody

  NOB1 mouse monoclonal antibody, clone OTI1C12 (formerly 1C12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOB1 (RC209537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOB1.(1:2000) Mouse IgG1 OTI1C12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NOB1 antibody

  NOB1 mouse monoclonal antibody, clone OTI4H3 (formerly 4H3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOB1 (RC209537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOB1.(1:2000) Mouse IgG1 OTI4H3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NOB1 antibody

  NOB1 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOB1 (RC209537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOB1.(1:2000) Mouse IgG1 OTI2F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

NOB1 - human gene knockout kit via CRISPR

NOB1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NOB1 - human gene knockout kit via CRISPR

NOB1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NOB1 - human gene knockout kit via CRISPR

NOB1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NOB1 - human gene knockout kit via CRISPR

NOB1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NOB1

  Recombinant protein of human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1)  
NOB1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NOB1 (1-412, His-tag)

NOB1 Human Purified > 80 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

NOB1 (1-412, His-tag)

NOB1 Human Purified > 80 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

NOB1 overexpression lysate

NOB1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NOB1 (mGFP-tagged) - Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1), 200 uL, >10^7 TU/mL

Lenti ORF particles, NOB1 (mGFP-tagged) - Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1), 200 uL, >10^7 TU/mL  
200 µl / €1,010.00
  OriGene Technologies, Inc.

Lenti ORF particles, NOB1 (Myc-DDK tagged) - Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, NOB1 (Myc-DDK tagged) - Human NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) (NOB1) , 200 uL, >10^7 TU/mL  
200 µl / €900.00
  OriGene Technologies, Inc.

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