NOB1 antibody

Principal name

NOB1 antibody

Alternative names for NOB1 antibody

ART4, NOB1P, PSMD8BP1, MSTP158, ART-4, RNA-binding protein NOB1,Phosphorylation regulatory protein HP-10

SwissProt ID

Q9ULX3 (Human)

Gene ID

28987 (NOB1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB)

Background of NOB1 antibody

In yeast, over 200 protein and R cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rR transcript into the mature 18S, 5.8S, and 25S rRs, folding of the rR, and binding of ribosomal proteins and 5S R. Nob1 is involved in pre-rR processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rR intermediate at cleavage site D to produce the mature 18S rR (Laman and Karbstein, 2009 [PubMed 19706509]).[supplied by OMIM, Nov 2010]. Transcript Variant: This variant (2) lacks an interl exon, compared to variant 1. This variant is represented as non-coding due to the presence of an upstream ORF that is predicted to interfere with translation of the longest ORF; translation of the upstream ORF renders the transcript a candidate for nonsense-mediated mR decay (NMD). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additiol publications. ##Evidence-Data-START## Transcript exon combition :: BG386829.1 [ECO:0000332] Rseq introns :: mixed/partial sample support ERS025081, ERS025082 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

General readings

Zhang Y., et al. (2005) Kidney Int. 79:1138-1148Daub H., et al. (2008) Mol. Cell. 31:438-448

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NOB1 antibody

WB Suggested Anti-NOB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NOB1 antibody

WB Suggested Anti-NOB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NOB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOB1 (RC209537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOB1.(1:2000) Mouse IgG1 OTI1C12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NOB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOB1 (RC209537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOB1.(1:2000) Mouse IgG1 OTI4H3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NOB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOB1 (RC209537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOB1.(1:2000) Mouse IgG1 OTI2F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NOB1 antibody

Transfected 293T; WB Suggested Anti-NOB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; NOB1 antibody - N-terminal region (ARP54975_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

NOB1 antibody

Human Liver; WB Suggested Anti-NOB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Liver; NOB1 antibody - C-terminal region (ARP54976_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NOB1 antibody

Western Blot: NOB1 Antibody [NBP1-55404] - This Anti-NOB1 antibody was used in Western Blot of Transfected 293T tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

NOB1 antibody

Western Blot: NOB1 Antibody [NBP1-56462] - This Anti-NOB1 antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NOB1

NOB1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NOB1 (1-412, His-tag)

NOB1 Human Purified > 80 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

NOB1 (1-412, His-tag)

NOB1 Human Purified > 80 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

NOB1

NOB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NOB1

NOB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NOB1 overexpression lysate

NOB1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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