NOS1 antibody

Principal name

NOS1 antibody

Alternative names for NOS1 antibody

bNOS, nNOS, NOS type I, Neuronal NOS, Constitutive NOS, NC-NOS, Nitric Oxide Synthase

SwissProt ID

O19132 (Rabit), P29475 (Human), P29476 (Rat), Q29498 (Sheep), Q9Z0J4 (Mouse)

Gene ID

4842 (NOS1), 18125 (Nos1), 24598

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse), Bov (Bovine), Dros (Drosophila), Por (Porcine), Rb (Rabbit), Hst (Hamster), GP (Guinea Pig), Can (Canine), Gt (Goat), Fe (Feline), Prim (Primate)

Available hosts

Rabbit, Guinea Pig, Mouse, Goat, Sheep

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of NOS1 antibody

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.
bNOS and eNOS share approximately 50% amino acid sequence homology and their enzymatic activity depends on binding to the calcium/calmodulin complex. iNOS, however, is tightly bound to calmodulin and acts independently of calcium levels. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short time. In vascular endothelial cells NOS appears to modulate vasotension and platelet aggregation. bNOS is found in neurons, peripheral nerve cells, macula densa, and pancreatic islet cells. Alternate splicing specifically regulates bNOS in striated muscle. The translated protein, bNOS mu, is 34 amino acid residues larger than bNOS from brain.

General readings

This product has been used in:Xue C et al. Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia. Am J Physiol 267:L667-78 (1994). PubMed PMID: 7528981
Rengasamy A et al. Immunohistochemical demonstration of a paracrine role of nitric oxide in bronchial function. Am J Physiol 267:L704-11 (1994). PubMed PMID: 7528982
General / background references:Nathan C Nitric oxide as a secretory product of mammalian cells. FASEB J 6:3051-64 (1992). PubMed PMID: 1381691

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NOS1 (C-term) antibody

Anti-NOS1 antibody (0.1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt C, E, ICC/IF, WB
20 µg / €200.00
  Acris Antibodies GmbH

NOS1 (C-term) antibody

Anti-NOS1 antibody (0.1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt C, E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOS1 antibody

Western blot analysis of extracts from RAW264.7 cells, treated with INF (2500u/ml, 10mins), using n-NOS (Ab-852) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS1 antibody

Western blot analysis of nNOS expression in INF treated RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS1 antibody

Western blot analysis of extracts of mouse brain cell line, using NOS1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOS1 antibody

Western blot analysis of nNOS expression in HeLa cells Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOS1 (1-181) antibody

NOS1 Mouse IgG1 N1 Purified Gt, Hu, Por, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

NOS1 antibody

Western blot (WB) analysis of NOS1 antibody (Cat.-No.: AP06622PU-N) in extracts from A549 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NOS1 (1422-1433) antibody

Formalin-Fixed Paraffin-Embedded Human skeletal muscle stained with nNOS / NOS1 antibody AP08236PU-N Rabbit IgG Aff - Purified Bt, Can, Chk, Elephant, Eq, Hu, Mky, Ms, Prim, Rb, Rt, Xen, Ze ICC/IF, IP, P, WB
0.1 ml / €470.00
  Acris Antibodies GmbH

NOS1 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using NOS1 Antibody Cat.-No AP09502PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

NOS1 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using NOS1 Antibody Cat.-No AP09502PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

NOS1 (C-term) antibody

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with NOS1 antibody AP22874PU-N at 20 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NOS1 (1418-1434) antibody

NOS1 Polyclonal Antibody Rabbit IgG Aff - Purified Hu, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

NOS1 pSer852 antibody

Western blot analysis of extracts from A549 tissue using n-NOS (Phospho-Ser852) antibody AP55924PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

NOS1 pSer852 antibody

Western blot analysis of extracts from A549 tissue using n-NOS (Phospho-Ser852) antibody AP55924PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

NOS1 antibody

NOS1 polyclonal antibody (A01), Lot # 060105JC01 Western Blot analysis of NOS1 expression in SJCRH30 ( Cat # L027V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NOS1 antibody

NOS1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

NOS1 antibody

NOS1 Mouse IgG1 N1 Gt, Hu, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

NOS1 antibody

NOS1 polyclonal antibody ( Cat # PAB6086 ) staining ( 0.3 µg/ml ) of human muscle extracts ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NOS1 antibody

Immunohistochemical staining of Nos1/Nos2 on formalin fixed, paraffin embedded human placenta with Nos1/Nos2 polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

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