NOS2 antibody

Principal name

NOS2 antibody

Alternative names for NOS2 antibody

NOS2A, iNOS, NOSII, NOS-II, Inducible NO synthase, NOS type II, Hepatocyte NOS

SwissProt ID

O19114 (Rabit), O54705 (Cavpo), O62699 (Canfa), P29477 (Mouse), P35228 (Human), P79290 (Pig), Q06518 (Rat), Q27995 (Bovin), Q64558 (Rat), Q90703 (Chick)

Gene ID

4843 (NOS2), 18126 (Nos2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP)

Background of NOS2 antibody

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

General readings

This product has been used in:Sharshar T et al. Apoptosis of neurons in cardiovascular autonomic centres triggered by inducible nitric oxide synthase after death from septic shock. Lancet 362:1799-805 (2003). PubMed PMID: 14654318

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NOS2 antibody

Western blot analysis of extracts from NIH/3T3 cells, using iNOS antibody (#TA314265).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS2 antibody

Western Blot: iNOS Antibody (4E5) [TA336918] - Western blot analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa) Mouse IgG1 4E5 Purified Hu, Ms E, F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS2 antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NOS2 mouse mAb with DAB staining. Mouse IgG1 4E5 Purified Hu, Ms E, F, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

NOS2 antibody

Formalin-fixed and paraffin-embedded human liver tissue (4 μm) stained with anti-iNOS (DB 003) antibody shows strong positive immunostaining of hepatocytes. Rabbit K13-A Aff - Purified Hu, Ms, Rt C, P
0.1 ml / €320.00
  DB Biotech, spol. s r.o.

Sample sizes available

NOS2 antibody

Formalin-fixed and paraffin-embedded human liver tissue (4 μm) stained with anti-iNOS (DB 003) antibody shows strong positive immunostaining of hepatocytes. Rabbit K13-A Aff - Purified Hu, Ms, Rt C, P
0.2 ml / €440.00
  DB Biotech, spol. s r.o.

Sample sizes available

NOS2 antibody

Formalin-fixed and paraffin-embedded human liver tissue (4 μm) stained with anti-iNOS (DB 003) antibody shows strong positive immunostaining of hepatocytes. Rabbit K13-A Aff - Purified Hu, Ms, Rt C, P
0.5 ml / €550.00
  DB Biotech, spol. s r.o.

Sample sizes available

NOS2 antibody

Formalin-fixed and paraffin-embedded human liver tissue (4 μm) stained with anti-iNOS (DB 003) antibody shows strong positive immunostaining of hepatocytes. Rabbit K13-A Aff - Purified Hu, Ms, Rt C, P
1 ml / €870.00
  DB Biotech, spol. s r.o.

Sample sizes available

NOS2 (C-term) antibody

AM21044PU iNOS antibody staining of Human Lung. Rabbit IgG SP126 Purified Hu P
0.5 ml / €510.00
  Acris Antibodies GmbH

NOS2 (C-term) antibody

AM21044PU iNOS antibody staining of Human Lung. Rabbit IgG SP126 Purified Hu P
1 ml / €730.00
  Acris Antibodies GmbH

NOS2 (C-term) antibody

AM21044PU iNOS antibody staining of Human Lung. Rabbit IgG SP126 Purified Hu P
0.1 ml / €290.00
  Acris Antibodies GmbH

NOS2 antibody

Human lung stained with anti-iNOS antibody Rabbit IgG Purified Hu, Ms P
0.5 ml / €360.00
  Acris Antibodies GmbH

NOS2 antibody

Human lung stained with anti-iNOS antibody Rabbit IgG Purified Hu, Ms P
1 ml / €440.00
  Acris Antibodies GmbH

NOS2 antibody

Human lung stained with anti-iNOS antibody Rabbit IgG Purified Hu, Ms P
0.1 ml / €180.00
  Acris Antibodies GmbH

NOS2 antibody

Western blot analysis of Thp-1 cells (Lanes 1 and 2: 50ug cell lysate; Lane 3: 75ug cell lysate) with anti-NOS2A antibody. (Predicted band size: 131kDa; observed band size: 120kDa) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS2 antibody

Western blot analysis of extracts from 3T3 cells, using iNOS (Ab-151) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS2 (Center) antibody

NOS2A Antibody (Center) (Cat. #TA324433) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NOS2A antibody detected the NOS2A protein (arrow). Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NOS2 antibody

Western blot analysis of iNOS expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS2 antibody

Western blot analysis on NIH-3T3 cell lysate using iNOS Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOS2 antibody

Western blot analysis of iNOS expression in NIH/3T3 cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOS2 antibody

Anti-iNOS (DB 003). Western blot of i(n)NOS in mouse brain crude lysate (50μg of protein loaded). Rabbit K13-A Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 ml / €430.00
  DB Biotech, spol. s r.o.

Sample sizes available

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