NOS3 antibody

Principal name

NOS3 antibody

Alternative names for NOS3 antibody

Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS, Nitric oxide synthase, endothelial

SwissProt ID

P29473 (Bovin), P29474 (Human), P70313 (Mouse), P79209 (Sheep), Q28969 (Pig), Q62600 (Rat)

Gene ID

4846 (NOS3), 287024, 18127 (Nos3)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), Mky (Monkey), Sh (Sheep), Bt (Bat), GP (Guinea Pig), Hst (Hamster), Prim (Primate), Rb (Rabbit), Bear

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of NOS3 antibody

Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).
eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.

General readings

Yanamandra, K., et al. Ophthalmic Genet. 31(4):173-177(2010)
Bambha, K., et al. Mayo Clin. Proc. 85(9):814-820(2010)
Shin, S.J., et al. Eur. J. Obstet. Gynecol. Reprod. Biol. 152(1):64-67(2010)
Kim, S.M., et al. Kardiol Pol 68(8):920-926(2010)
Dafni, C., et al. BMC Med. Genet. 11, 133 (2010).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NOS3 antibody

Western Blot: eNOS Antibody (6H2) [TA336799] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml. Mouse IgG1 6H2 Purified Hu C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 (C-term) antibody

Human placenta stained with Anti-eNOS antibody (Cat#AP15681PU) Rabbit Purified Bov, Can, Hu, Ms, Por, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

NOS3 (C-term) antibody

Human placenta stained with Anti-eNOS antibody (Cat#AP15681PU) Rabbit Purified Bov, Can, Hu, Ms, Por, Rt P
1 ml / €450.00
  Acris Antibodies GmbH

NOS3 (C-term) antibody

Human placenta stained with Anti-eNOS antibody (Cat#AP15681PU) Rabbit Purified Bov, Can, Hu, Ms, Por, Rt P
0.1 ml / €180.00
  Acris Antibodies GmbH

NOS3 antibody

Western blot analysis of extracts from COLO cells, using eNOS (Ab-494) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Western blot analysis of extracts from Jurkat cells and HepG2 cells, using eNOS (Phospho-Thr495) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Western blot analysis of extracts from Jurkat cells, using eNOS (Ab-615) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Predicted band size: 140 kDa . Positive control: Rat heart, Mouse heart and Rat kidney tissue lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: Rat heart lysate Lane2: Mouse heart lysate Lane3: Rat kidney tissue lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Western blot analysis on K562 cell lysate using eNOS Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Western blot analysis of eNOS expression in Insulin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Western blot analysis of eNOS expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

NOS3 Rabbit IgG Purified
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Human Placenta stained with anti-Nitric Oxide Synthase-Endothelial (eNOS) antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Human Placenta stained with anti-Nitric Oxide Synthase-Universal (uNOS) antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOS3 (RC209228, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOS3. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOS3 (RC209228, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOS3. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOS3 pSer1177 antibody

Immunoblot Analysis: Whole cell lysates of untreated (Lane 1) and pervanadate-treated (Lane 2) bend 3 cells were separated by SDS-PAGE and blotted to a PVDF membrane. The immunoblot was probed with mab eNOS Antibody Cat.-No AM00098PU-N (Clone 15E2) at 1 μg/ml for 1h at 15-22°C and developed by ECL (exposure time: 3min). Mab eNOS-15E2 recognizes eNOS pSer1177 at 140 kDa. Mouse IgG1 15E2 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

NOS3 antibody

Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb with DAB staining. Mouse IgG1 6H2 Ascites Hu E, P
0.1 ml / €400.00
  Acris Antibodies GmbH

NOS3 antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 6H2 Ascites Hu E, P
50 µl / €470.00
  Acris Antibodies GmbH

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