NOS3 antibody

Principal name

NOS3 antibody

Alternative names for NOS3 antibody

Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS, Nitric oxide synthase, endothelial

SwissProt ID

P29473 (Bovin), P29474 (Human), P70313 (Mouse), P79209 (Sheep), Q28969 (Pig), Q62600 (Rat)

Gene ID

4846 (NOS3), 287024

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Mky (Monkey), GP (Guinea Pig), Prim (Primate), Sh (Sheep), Bear, Eq (Equine), Bt (Bat), Hst (Hamster), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of NOS3 antibody

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

General readings

Yanamandra, K., et al. Ophthalmic Genet. 31(4):173-177(2010)
Bambha, K., et al. Mayo Clin. Proc. 85(9):814-820(2010)
Shin, S.J., et al. Eur. J. Obstet. Gynecol. Reprod. Biol. 152(1):64-67(2010)
Kim, S.M., et al. Kardiol Pol 68(8):920-926(2010)
Dafni, C., et al. BMC Med. Genet. 11, 133 (2010).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NOS3 antibody

Western Blot: eNOS Antibody (6H2) [TA336799] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml. Mouse IgG1 6H2 Purified Hu C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 (C-term) antibody

Human placenta stained with Anti-eNOS antibody (Cat#AP15681PU) Rabbit Purified Bov, Can, Hu, Ms, Por, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

NOS3 (C-term) antibody

Human placenta stained with Anti-eNOS antibody (Cat#AP15681PU) Rabbit Purified Bov, Can, Hu, Ms, Por, Rt P
1 ml / €440.00
  Acris Antibodies GmbH

NOS3 (C-term) antibody

Human placenta stained with Anti-eNOS antibody (Cat#AP15681PU) Rabbit Purified Bov, Can, Hu, Ms, Por, Rt P
0.1 ml / €180.00
  Acris Antibodies GmbH

NOS3 antibody

Western blot analysis of extracts from COLO cells, using eNOS (Ab-494) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Western blot analysis of extracts from Jurkat cells, using eNOS (Ab-615) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Predicted band size: 140 kDa . Positive control: Rat heart, Mouse heart and Rat kidney tissue lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: Rat heart lysate Lane2: Mouse heart lysate Lane3: Rat kidney tissue lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Western blot analysis on K562 cell lysate using eNOS Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Western blot analysis of eNOS expression in Insulin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

Western blot analysis of eNOS expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOS3 (RC209228, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOS3. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOS3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NOS3 (RC209228, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOS3. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOS3 pSer1177 antibody

Immunoblot Analysis: Whole cell lysates of untreated (Lane 1) and pervanadate-treated (Lane 2) bend 3 cells were separated by SDS-PAGE and blotted to a PVDF membrane. The immunoblot was probed with mab eNOS Antibody Cat.-No AM00098PU-N (Clone 15E2) at 1 μg/ml for 1h at 15-22°C and developed by ECL (exposure time: 3min). Mab eNOS-15E2 recognizes eNOS pSer1177 at 140 kDa. Mouse IgG1 15E2 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

NOS3 antibody

Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb with DAB staining. Mouse IgG1 6H2 Ascites Hu E, P
0.1 ml / €400.00
  Acris Antibodies GmbH

NOS3 antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 6H2 Ascites Hu E, P
50 µl / €470.00
  Acris Antibodies GmbH

NOS3 antibody

Western blot (WB) analysis of NOS3 antibody (Cat.-No.: AP01459PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NOS3 pThr494 antibody

Western blot (WB) analysis of NOS3 (pThr494) antibody (Cat.-No.: AP01888PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NOS3 (C-term) antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

NOS3 pSer1177 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Ser1177 eNOS antibody (#AP08011PU) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P
0.1 ml / €320.00
  Acris Antibodies GmbH

NOS3 pSer1177 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Ser1177 eNOS antibody (#AP08011PU) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P
50 µl / €240.00
  Acris Antibodies GmbH

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