NOTCH1 antibody

Principal name

NOTCH1 antibody

Alternative names for NOTCH1 antibody

Notch 1, hN1, TAN1, Neurogenic locus notch homolog protein 1

SwissProt ID

P46530 (Danre), P46531 (Human), Q01705 (Mouse), Q07008 (Rat)

Gene ID

4851 (NOTCH1), 30718 (notch1a), 18128

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Chk (Chicken), Ze (Zebrafish), African clawed frog, Por (Porcine)

Available hosts

Rabbit, Mouse, Hamster, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Functional assay (FN), Neutralisation (NEUT)

Background of NOTCH1 antibody

NOTCH1 is a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Sahlgren,C., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (17), 6392-6397

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NOTCH1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min). Mouse IgG2b OTI3E12 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

NOTCH1 antibody

Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with Biotin anti-Notch 1 Antibody (Cclone mN1A). Proteins were visualized using a Streptavidin-HRP and chemiluminescence detection. Mouse IgG1 mN1A Biotin Hu, Ms WB
0.1 mg / €270.00
  Acris Antibodies GmbH

NOTCH1 antibody

Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with Biotin anti-Notch 1 Antibody (Cclone mN1A). Proteins were visualized using a Streptavidin-HRP and chemiluminescence detection. Mouse IgG1 mN1A Biotin Hu, Ms WB
25 µg / €190.00
  Acris Antibodies GmbH

NOTCH1 antibody

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-NOTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500248, Dilution 1:50) Mouse IgG2b OTI4C9 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NOTCH1 antibody

Immunohistochemistry-Paraffin: Notch1 Antibody (3E12) [NBP1-48289] - Immunohistochemical staining of paraffin-embedded lung using anti-Notch1 mouse monoclonal antibody. Mouse IgG2b 3E12 Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

NOTCH1 (C-term) antibody

Ovarian carcinoma stained with Anti-Notch-1 antibody Rabbit Purified Hu, Ms, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

NOTCH1 (C-term) antibody

Ovarian carcinoma stained with Anti-Notch-1 antibody Rabbit Purified Hu, Ms, Rt P
1 ml / €520.00
  Acris Antibodies GmbH

NOTCH1 (C-term) antibody

Ovarian carcinoma stained with Anti-Notch-1 antibody Rabbit Purified Hu, Ms, Rt P
0.1 ml / €190.00
  Acris Antibodies GmbH

NOTCH1 antibody

Western blot analysis of extracts from NIH/3T3 cells, treated with etoposide (25µM, 1hour), using Notch 1 (cleaved-Val1744) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOTCH1 (Intra) antibody

anti-NOTCH 1 antibody was diluted 1:500 to detect NOTCH 1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Rabbit Serum Hu E, P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

NOTCH1 (N-term of NICD) antibody

Anti-Human NOTCH 1 (Cleaved N Terminal) was used at 1:500 to detect mouse Notch 1. Lane M: Marker. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular)-myc. Lane 4: N2 (mouse deleted extracellular)-myc. Lane 5: N2 (mouse intracellular)-myc. Lane 6: N3 (mouse deleted extracellular)-myc. Lane 7: N3 (mouse intracellular)-myc. Lane 8: N4 (mouse deleted extracellular)-myc. Lane 9: N4 (mouse intracellular)-myc. Lane 10: N1 (mouse deleted extracellular)(V to G)-myc. Rabbit Serum Hu, Ms, Rt E, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

NOTCH1 antibody

Predicted band size: 273kDa. Positive control: HT-29 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 60 ug Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 7 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOTCH1 antibody

Predicted band size: 273 kDa . Positive control: HT-29 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOTCH1 antibody

Western blot analysis of extracts of various cell lines, using NOTCH1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOTCH1 antibody

Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were Western Blot analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kD full-length NOTCH1 and 110-120 kD cleaved NOTCH 1 (NICD). Mouse IgG1 mN1A Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOTCH1 antibody

Western Blot: Notch1 Antibody (mN1A) [TA336494] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were Western Blot analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kD full-length NOTCH1 Mouse IgG1 mN1A Purified Hu, Ms C, F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOTCH1 antibody

Western Blot: Notch1 Antibody [TA336846] - WB analysis of Cleaved Notch1 in Jurkat cell lysate. Rabbit Purified Hu, Ms C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOTCH1 antibody

Western blot analysis of Notch 1 (Cleaved-Val1744) expression in NIH 3T3 cell extract treated with etoposide Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOTCH1 antibody

NOTCH1 Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOTCH1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NOTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808308)(1:150) Mouse IgG1 OTI2E7 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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