NOXO1 antibody

Principal name

NOXO1 antibody

Alternative names for NOXO1 antibody

P41NOX, SH3PXD5, NADPH oxidase organizer 1

SwissProt ID

Q8NFA2 (Human)

Gene ID

124056 (NOXO1)

Available reactivities

Hu (Human), Mky (Monkey), Can (Canine), Chimp (Chimpanzee), GP (Guinea Pig)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of NOXO1 antibody

The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner, producing reactive oxygen in various cells and tissues in response to growth factors, cytokines and calcium signals. The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2), together with the regulatory subunits p22phox, p47phox, p40phox, p67phox and the small GTPase RAC. The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex. In 1999, the first of the NOX homologues of gp91phox was described as NOX1. The enzyme was cloned from a colon epithelial cell complementary DNA library. When expressed in cells, NOX1 generated low amounts of ROS, but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later). Subsequently, NOX3 and NOX4 were cloned, and the latter was shown to generate high levels of ROS when expressed in cells. NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization, except that it lacks the auto-inhibitory region. NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization, except that it lacks one of the two SH3 domains that are present in p67phox. Co-transfection of NOX1, NOXO1 and NOXA1 results in marked ROS generation. Similar to p47phox, NOXO1 binds to p22phox, which is required for NOX1-dependent activity. NOXA1 has a well-conserved activation domain, implying a conserved mechanism for regulating the activity of the target NOX enzyme. (Lambeth JD. Nat Rev Immunol. 2004 Mar;4(3):181-9)

General readings

Banfi B, Clark RA, Steger K, Krause KH.
Two novel proteins activate superoxide generation by the NADPH oxidase NOX1.
J Biol Chem. 2003 Feb 7;278(6):3510-3.
PMID: 12473664

  1. Quick-Filters:
  2. Cited (1)

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NOXO1 (238-252) antibody

WB using Anti-NOXO1 antibody shows detection of a band ~50 kDa corresponding to human NOXO1 (arrowhead). Reactivity was observed in transfected human 293 cells and human HT-29 colon carcinoma cells (endogenous). A 1:1,000 dilution of the primary antibody was used for detection followed by secondary antibody reactivity. Specific band reactivity was competed away when the antibody was pre-incubated with the peptide immunogen (data not shown). Rabbit IgG Aff - Purified Can, Chimp, GP, Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOXO1 (C-term) antibody

TA302927 (10µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Hu P
0.1 mg / €325.00
  OriGene Technologies, Inc.

NOXO1 antibody

Sample (30 ug of whole cell lysate). A: HCT116 10% SDS PAGE. TA309051 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NOXO1 (C-term) antibody

Immunohistochemistry of Formalin-fixed, Paraffin-embedded human renal tubular epithelium after heat-induced antigen retrieval using anti-NOXO1 antibody cat.no. AP07536PU-N Goat Aff - Purified Hu, Prim E, P
50 µg / €470.00
  Acris Antibodies GmbH

NOXO1 (238-252) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Colon. Rabbit Aff - Purified Hu, Mky E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NOXO1 antibody

NOXO1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NOXO1 antibody

NOXO1 polyclonal antibody ( Cat # PAB6594 ) ( 10 µg/ml ) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Hu E, P
0.1 mg / €400.00
  Abnova Taiwan Corp.

NOXO1 antibody

Immunohistochemistry: NOXO1 Antibody [NB300-950] - NB300-950 (10ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

NOXO1 antibody

Western  Blot:  NOXO1 Antibody [NBP1-33653] – Western blot analysis of NOXO1 on HCT116 lysates using NBP1-33653 at a dilution of 1:1000. 30ug whole cell lysate loaded on 10% SDS PAGE gel. Rabbit IgG Hu WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NOXO1 (transcript variant b)

NOXO1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NOXO1 (transcript variant c)

NOXO1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NOXO1

NOXO1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NOXO1

NOXO1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NOXO1 overexpression lysate

NOXO1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

per page
  • LinkedIn