NPY receptor 2 / NPY2R antibody

Principal name

NPY receptor 2 / NPY2R antibody

Alternative names for NPY receptor 2 / NPY2R antibody

NPY2-R, NPY-Y2 receptor, Y2 receptor, Neuropeptide Y receptor 2

SwissProt ID

O02836 (Pig), P49146 (Human), P79113 (Bovin), P79211 (Sheep), P97295 (Mouse), Q9DDN6 (Chick), Q9Z2D5 (Cavpo)

Gene ID

4887 (NPY2R), 18167 (Npy2r)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Mky (Monkey)

Available hosts

Rabbit, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P)

Background of NPY receptor 2 / NPY2R antibody

Neuropeptide Y (NPY) is a 36-amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems. It belongs to the NPY family, together with peptide YY (PYY) and pancreatic polypeptide (PP). NPY is involved in regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction. NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R). Four of these, Y1, Y2, Y4, and Y5, are expressed in humans. The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus. Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on the high frequency and density of NPY receptors in steroid hormone-producing tumors, the NPY receptors might be of potential use in tumor magement. NPY2R is primarily expressed in neurons as a presyptic receptor. It is also strongly expressed in epithelia of the visceral tissues, including colon and kidney. NPY2R is involved in inhibition of neurotransmitter release mediated by NPY.5 Release of glutamate, the principal neuroexcitatory transmitter, is inhibited via the NPY2R receptor. Involvement of NPY2R in feeding behavior was also reported. PYY(3–36), an NPY2R agonist, acts to inhibit feeding via NPY2R.

General readings

1. Burdyga G, Varro A, Dimaline R, Thompson DG, Dockray GJ. Expression of cannabinoid CB1 receptors by vagal afferent neurons: kinetics and role in influencing neurochemical phenotype. Am J Physiol Gastrointest Liver Physiol. 2010 Jul;299(1):G63-9. doi: 10.1152/ajpgi.00059.2010. Epub 2010 Apr 29. PubMed PMID: 20430875.
2. Wang L, Gourcerol G, Yuan PQ, Wu SV, Million M, Larauche M, et al. Peripheral peptide YY inhibits propulsive colonic motor function through Y2 receptor in conscious mice. Am J Physiol Gastrointest Liver Physiol. 2010 Jan;298(1):G45-56. doi: 10.1152/ajpgi.00349.2009. Epub 2009 Nov 5. PubMed PMID: 19892938.
3. Burdyga G, de Lartigue G, Raybould HE, Morris R, Dimaline R, Varro A, et al. Cholecystokinin regulates expression of Y2 receptors in vagal afferent neurons serving the stomach. J Neurosci. 2008 Nov 5;28(45):11583-92. doi: 10.1523/JNEUROSCI.2493-08.2008. PubMed PMID: 18987194.
4. Brumovsky P, Stanic D, Shuster S, Herzog H, Villar M, Hökfelt T. Neuropeptide Y2 receptor protein is present in peptidergic and nonpeptidergic primary sensory neurons of the mouse. J Comp Neurol. 2005 Aug 29;489(3):328-48. PubMed PMID: 16025447.
5. Tu B, Jiao Y, Herzog H, Nadler JV. Neuropeptide Y regulates recurrent mossy fiber synaptic transmission less effectively in mice than in rats: Correlation with Y2 receptor plasticity. Neuroscience. 2006 Dec 28;143(4):1085-94. Epub 2006 Oct 4. PubMed PMID: 17027162.
6. Andres Acosta1, Maria D. Hurtado1, Oleg Gorbatyuk, Michael La Sala, David Duncan,
George Aslanidi, Martha Campbell-Thompson, Lei Zhang, Herbert Herzog, Antonis
Voutetakis, Bruce J. Baum, Sergei Zolotukhin. Salivary PYY: A Putative Bypass to Satiety.
PLoS ONE 6(10): e26137. doi:10.1371/journal.pone.0026137. Received: July 22, 2011; Accepted: September 20, 2011; Published: October 10, 2011
7. Davor Stanić, Jan Mulder, Masahiko Watanabe, Tomas Hökfelt. Characterization of NPY Y2 receptor protein expression in the mouse brain. II. Coexistence with NPY, the Y1 receptor, and other neurotransmitter-related molecules. DOI: 10.1002/cne.22608. The Journal of Comparative Neurology. Volume 519, Issue 7, pages 1219–1257, 1 May 2011.
This references use of NPY Y2 antibody for western blot analysis using mouse hippocampal lysates where a band is seen at 44 kDa.

Items 1 to 20 of 31 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NPY receptor 2 / NPY2R antibody

TA303196 (0.1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NPY receptor 2 / NPY2R antibody

Western blot analysis of extracts from HepG2 cells, using NPY2R antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NPY receptor 2 / NPY2R antibody

Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using NPY2R antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NPY receptor 2 / NPY2R antibody

Western blot analysis of rat hippocampus and rat whole brain lysates: 1, 2. Anti-Neuropeptide Y2 Receptor antibody, (1:200). 3, 4. Anti-Neuropeptide Y2 Receptor antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

NPY receptor 2 / NPY2R antibody

Western blot (WB) analysis of NPY2-R antibody (Cat.-No.: AP01323PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NPY receptor 2 / NPY2R Control Peptide

NPY receptor 2 / NPY2R
0.25 mg / €250.00
  Acris Antibodies GmbH

NPY receptor 2 / NPY2R (C-term) antibody

NPY receptor 2 / NPY2R Rabbit Aff - Purified Hu E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

NPY receptor 2 / NPY2R Control Peptide

NPY receptor 2 / NPY2R Purified
0.1 mg / €150.00
  Acris Antibodies GmbH

NPY receptor 2 / NPY2R antibody

NPY Y2 receptor immunoreactivity in Mouse dorsal root ganglion of wild type (A) and NPY Y2 receptor Knockout (B) animals.  Note that  unspecific staining is observed in thick, possibly myelinated fibers.  Image courtesy of Dr. Tomas Hökfelt, Karolinska Institute. Rabbit IgG Aff - Purified Ms, Rt C, ICC/IF
0.1 ml / €380.00
  Acris Antibodies GmbH

NPY receptor 2 / NPY2R (N-term + C-term) antibody

Figure 1. NPY Y2R staining of Rat cortex using Cy3-conjugated Donkey anti-Rabbit secondary antibodies (Red color) and DAPI (Blue) as a nuclear counterstain. Rabbit IgG Serum Ms, Rt C, ICC/IF
0.1 ml / €350.00
  Acris Antibodies GmbH

NPY receptor 2 / NPY2R (N-term + C-term) antibody

Figure 1. NPY Y2R staining of Rat cortex using Cy3-conjugated Donkey anti-Rabbit secondary antibodies (Red color) and DAPI (Blue) as a nuclear counterstain. Rabbit IgG Serum Ms, Rt C, ICC/IF
50 µl / €240.00
  Acris Antibodies GmbH

NPY receptor 2 / NPY2R (N-term) antibody

Western blot analysis of NPY2R Antibody (N-term) (Cat. #AP52925PU-N) in mouse bladder tissue lysates (35µg/lane). DCN (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NPY receptor 2 / NPY2R antibody

IHC analysis of human testis. Using NPY2R polyclonal antibody ( Cat # PAB0289 ) at 10 µg/ml. Rabbit Hu, Mky C, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NPY receptor 2 / NPY2R antibody

The Npy2r polyclonal antibody ( Cat # PAB7213 ) ( 0.1 µg/ml ) staining of rat brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NPY receptor 2 / NPY2R antibody

Immunofluorescence analysis of A549 cells, using NPY2R Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

NPY receptor 2 / NPY2R antibody

Immunofluorescence analysis of A549 cells, using NPY2R Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

NPY receptor 2 / NPY2R antibody

Immunofluorescent analysis of NPY2R staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NPY receptor 2 / NPY2R antibody

Immunofluorescent analysis of NPY2R staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NPY receptor 2 / NPY2R antibody

Immunofluorescent analysis of NPY2R staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

NPY receptor 2 / NPY2R Control Peptide

of NPY Y2 receptor immunoreactivity in mouse dorsal root ganglion of wild type (A) and NPY Y2 receptor Knockout (B) animals.  Note that  unspecific staining is observed in thick, possibly myelinated fibers.  Image courtesy of Dr. Tomas Hökfelt, Karolinska Institute. Purified
0.1 mg / €150.00
  Neuromics Antibodies

Items 1 to 20 of 31 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn