NQO1 antibody

Principal name

NQO1 antibody

Alternative names for NQO1 antibody

DIA4, NMOR1, NAD(P)H dehydrogenase [quinone] 1, Quinone reductase 1, NAD(P)H:quinone oxidoreductase 1, DT-diaphorase, Azoreductase, Phylloquinone reductase, Menadione reductase

SwissProt ID

P05982 (Rat), P15559 (Human), Q64669 (Mouse)

Gene ID

1728 (NQO1)

Available reactivities

Can (Canine), Hu (Human), Mky (Monkey), Rt (Rat), Ms (Mouse), Por (Porcine), Bov (Bovine), Prim (Primate), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of NQO1 antibody

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinones thus preventing the formation of reactive oxygen species. Mutations in the gene for NQO1 have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD).
NQO1 levels have been shown to be raised in may tumour types. This has led to a search for anti-tumour agents that can be activated by NQO1 resulting in specific targetting of these compounds to the tumour site. Many cytotoxic drugs contain the quinone moiety, such as the benzoquinone group, which can be activated by NQO1 through the reduction into hydroquinone [which in this case can auto-oxidize and lead to the formation of free radicals and alkylating species]. However, the rat enzyme has been shown to be significantly more effective in activating some of these compounds than the human and mouse enzymes. This indicates that the available cytotoxic drugs are better substrates for the rat enzyme and are not the most ideal prodrugs for activation by NQO1 in human tumors.

General readings

Asher G et al. NQO1 stabilizes p53 through a distinct pathway. Proc Natl Acad Sci U S A 99:3099-104 (2002). PubMed PMID: 11867746
1. Singh, S., Zahid, M., Gaikwad, N., Chakravarti, D., Cavalieri, E. L., and Rogan, E. G. (2006) The effect of NAD(P)H:quinone oxidoreductase 1 polymorphisms on estrogen metabolism in relation to initiation of breast cancer, 2006, 835-b-.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NQO1 antibody

Sample (30 ug of whole cell lysate). A: Molt-4 . B: Raji. 12% SDS PAGE. TA308820 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NQO1 antibody

Western blot analysis of extracts of various cell lines, using NQO1 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

NQO1 antibody

Immunohistochemical analysis of paraffin-embedded testis tissues using NQO1 mouse mAb with DAB staining. Mouse IgG1 4D12 Ascites Hu E, F, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

NQO1 antibody

Western blot analysis using NQO1 mAb against human NQO1 (AA: 134-274) recombinant protein. (Expected MW is 41.3 kDa) Mouse IgG1 1A11 Ascites Hu E, F, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

NQO1 antibody

NQO1 MaxPab rabbit polyclonal antibody. Western Blot analysis of NQO1 expression in human stomach. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NQO1 antibody

Immunofluorescence of monoclonal antibody to NQO1 on HepG2 cell. [antibody concentration 10 ug/ml] Mouse IgG1 1E3-A6 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NQO1 (C-term) antibody

TA302678 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

NQO1 (Isoform A) antibody

AP23673PU-N NQO1 antibody staining of Human Kidney lysate at 0.3 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

NQO1 antibody

Detection of NQO1 in A549 lysates. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second Mouse IgG1 A180 Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NQO1 (C-term) antibody

TA302678 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NQO1 antibody

Predicted band size: 27 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NQO1 antibody

Predicted band size: 27 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NQO1 antibody

Western blot analysis of NQO1 antibody (Center) (Cat.#TA324929) in NCI-H460 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu F, ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NQO1 antibody

Western Blot: NQO1 Antibody [TA336962] - Western blot analysis of NQO1 in human spleen lysate using TA336962 at A) 1:1000 and B) 1:500. Rabbit Purified Can, Hu, Prim P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NQO1 antibody

WB Suggested Anti-NQO1 Antibody; Titration: 1.0 ug/ml; Positive Control: 721_B Whole Cell There is BioGPS gene expression data showing that NQO1 is expressed in 721_B Rabbit IgG Purified Bov, Can, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NQO1 antibody

NQO1 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

NQO1 antibody

Western Blot analysis of NQO1 expression in transfected 293T cell line (H00001728-T01) by NQO1 MaxPab polyclonal antibody.

Lane 1: NQO1 transfected lysate(30.14 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

NQO1 antibody

NQO1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

NQO1 antibody

Western blot analysis of NQO1 in A549 lysates ( Lane 1 : 0.5 µg/ml. Lane 2 : 2 µg/ml ) with NQO1 monoclonal antibody, clone A180 ( Cat# MAB2302 ). Mouse IgG1 A180 Hu, Rt ICC/IF, IP, P, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

NQO1 antibody

NQO1 polyclonal antibody ( Cat # PAB6134 ) staining ( 1 µg/ml ) of human lung lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

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