NR1D1 antibody
Principal name
NR1D1 antibody
Alternative names for NR1D1 antibody
EAR1, HREV, THRAL, Nuclear receptor subfamily 1 group D member 1, V-erbA-related protein EAR-1, Rev-erbA-alpha
SwissProt ID
P20393 (Human)
Gene ID
9572 (NR1D1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of NR1D1 antibody
Rev-erbA alpha, a NR1 Thyroid Hormone-Like Receptor, is encoded by the same genomic locus as, but transcribed from the opposite strand of, Thyroid Hormone Receptor Alpha (TR Alpha). Rev-erbA alpha is a target of Nuclear Receptor ROR Alpha, and a transcription regulator that has been shown to affect myocyte differentiation, adipogenesis, and lipoprotein metabolism. Recently Reb-erbA alpha has been shown to affect circadian rhythms by regulating cyclic Bmal1 transcription. Mice lacking Rev-erbA alpha show abnormal postnatal cerebellar development.
Rev-erbA alpha expression has been documented in human skeletal muscle and a variety of mouse and rat tissues. ESTs have been isolated from human tissue libraries, including cancerous adrenal, blood, brain, breast, colon, duodenum, fetus, head/neck, kidney, lung, skeletal muscle, skin, synovium, and uterus, and normal brain, breast, colon, eye, heart, pancreas, pituitary, rostate, skeletal muscle, skin, testis, and thyroid.
General readings
Wang,J. (2008) Mol. Cell. Biol. 28 (7), 2213-2220
Immunoperoxidase of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to NR1D1 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot analysis of NR1D1 over-expressed 293 cell line, cotransfected with NR1D1 Validated Chimera RNAi ( Cat # H00009572-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D1 monoclonal antibody (M02), clone 4F6 (Cat # H00009572-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
NR1D1 monoclonal antibody (M02), clone 4F6 Western Blot analysis of NR1D1 expression in Hela NE ( Cat # L013V3 ).
NR1D1 monoclonal antibody (M02), clone 4F6. Western Blot analysis of NR1D1 expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot analysis of NR1D1 expression in transfected 293T cell line by NR1D1 monoclonal antibody (M02), clone 4F6.Lane 1: NR1D1 transfected lysate(66.8 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged NR1D1 is approximately 1ng/ml as a capture antibody.
NR1D1 monoclonal antibody (M05), clone 5H6 Western Blot analysis of NR1D1 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged NR1D1 is approximately 3ng/ml as a capture antibody.
NR1D1 monoclonal antibody (M01), clone 4C9. Western Blot analysis of NR1D1 expression in human stomach.
NR1D1 monoclonal antibody (M01), clone 4C9. Western Blot analysis of NR1D1 expression in human stomach.
Detection limit for recombinant GST tagged NR1D1 is approximately 3ng/ml as a capture antibody.
NR1D1 monoclonal antibody (M17), clone 3G5. Western Blot analysis of NR1D1 expression in human skeletal muscle.
NR1D1 monoclonal antibody (M17), clone 3G5. Western Blot analysis of NR1D1 expression in human kidney.
Transfected 293T; WB Suggested Anti-NR1D1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; NR1D1 antibody - N-terminal region (ARP33733_P050) in Transfected 293T cells using Western Blot
Transfected 293T; WB Suggested Anti-NR1D1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; NR1D1 antibody - middle region (ARP45634_P050) in Transfected 293T cells using Western Blot
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to NR1D1 on HeLa cell (antibody concentration 10 µg/ml).
NR1D1 monoclonal antibody, clone 4F6. Western Blot analysis of NR1D1 expression in NIH/3T3.
NR1D1 monoclonal antibody clone 4F6 Western Blot analysis of NR1D1 expression in Hela NE.
Detection limit for recombinant GST tagged NR1D1 is approximately 1 ng/ml as a capture antibody.
Anti-NR1D1 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D1 antibody IHC of human brain, thalamus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D1 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 4 ug/ml.
Anti-NR1D1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 4 ug/ml.
WB Suggested Anti-NR1D1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T
WB Suggested Anti-NR1D1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (NR1D1 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (NR1D1 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM20975PU-N | NR1D1 antibody |
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Mouse | IgG1 | 4F6 | Hu, Ms | E, ICC/IF, P, WB |
50 µg /
€530.00
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| Acris Antibodies GmbH | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA317083 | NR1D1 antibody |
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| NR1D1 Rabbit Polyclonal (Internal) Antibody | |||||||
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Rabbit | Aff - Purified | Hu | P |
50 µg /
€400.00
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| OriGene Technologies, Inc. | |||||||
| TA317084 | NR1D1 antibody |
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| NR1D1 Rabbit Polyclonal (Internal) Antibody | |||||||
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Rabbit | IgG | Purified | Bt, Eq, Hu, Mky, Por, Rb |
50 µg /
€400.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA317085 | NR1D1 antibody |
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| NR1D1 Rabbit Polyclonal (Internal) Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, Gorilla, Hst, Hu, Mky, Ms, Por, Rb, Rt |
50 µg /
€400.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA334242 | NR1D1 antibody |
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| Rabbit Polyclonal Anti-NR1D1 Antibody | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, Hu, Ms, Rb, Sh | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA338008 | NR1D1 antibody |
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| Rabbit Polyclonal Anti-NR1D1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Sh | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350237 | NR1D1 antibody |
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| Rabbit Polyclonal Anti-NR1D1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN208915 | NR1D1 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN208915BN | NR1D1 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208915LP | NR1D1 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208915RB | NR1D1 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC208915L2V | Lenti ORF particles, NR1D1 (mGFP-tagged) - Human nuclear receptor subfamily 1, group D, member 1 (NR1D1), 200 uL, >10^7 TU/mL |
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200 µl /
€1,050.00
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| OriGene Technologies, Inc. | ||
| RC208915L1V | Lenti ORF particles, NR1D1 (Myc-DDK tagged) - Human nuclear receptor subfamily 1, group D, member 1 (NR1D1) , 200 uL, >10^7 TU/mL |
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200 µl /
€940.00
|
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| OriGene Technologies, Inc. | ||
